Anti-Aromatase antibody (ab35604)
Key features and details
- Rabbit polyclonal to Aromatase
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Aromatase antibody
See all Aromatase primary antibodies -
Description
Rabbit polyclonal to Aromatase -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Dog -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Aromatase.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab35604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa). |
Target
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Function
Catalyzes the formation of aromatic C18 estrogens from C19 androgens. -
Tissue specificity
Brain, placenta and gonads. -
Involvement in disease
Defects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1588 Human
- Omim: 107910 Human
- SwissProt: P11511 Human
- Unigene: 260074 Human
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Alternative names
- ARO antibody
- ARO1 antibody
- Aromatase antibody
see all
Images
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Anti-Aromatase antibody (ab35604) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 150 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab35604 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab35604 has been referenced in 16 publications.
- de Andrés PJ et al. Hormonal Homologies between Canine Mammary Cancer and Human Breast Cancer in a Series of Cases. Vet Sci 9:N/A (2022). PubMed: 36006309
- Bose HS et al. Regulation of Estradiol Synthesis by Aromatase Interacting Partner in Breast (AIPB). Mol Cell Biol 41:e0035721 (2021). PubMed: 34460330
- Wang X et al. Extracellular Vesicles Inhibit Proliferation and Invasion of Ovarian Endometrial Stromal Cells and Their Expression of SF-1, ERβ, and Aromatase. Front Endocrinol (Lausanne) 12:666195 (2021). PubMed: 34531822
- Zarate-Perez F et al. Biophysical characterization of Aptenodytes forsteri cytochrome P450 aromatase. J Inorg Biochem 184:79-87 (2018). WB . PubMed: 29684698
- Ke J et al. Prostaglandin E2 triggers cytochrome P450 17a hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes invasion in endometrial cancer. Oncol Lett 16:4577-4585 (2018). PubMed: 30214592