Recombinant Anti-GBP1 antibody [EPR8285] - BSA and Azide free (ab240050)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8285] to GBP1 - BSA and Azide free
- Suitable for: ICC/IF, IHC-P, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GBP1 antibody [EPR8285] - BSA and Azide free
See all GBP1 primary antibodies -
Description
Rabbit monoclonal [EPR8285] to GBP1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A549 and HeLa (Untreated or treated with 10ng/ml IFN-? for 24 hours) cell lysates. ICC/IF: IFN-? induced HeLa cells. IHC-P: Human spleen tissue.
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General notes
ab240050 is the carrier-free version of ab131255.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 2.00 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8285 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
Target
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Function
Binds GTP, GDP and GMP. -
Sequence similarities
Belongs to the GBP family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2633 Human
- Omim: 600411 Human
- SwissProt: P32455 Human
- Unigene: 62661 Human
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Alternative names
- GBP 1 antibody
- GBP-1 antibody
- GBP1 antibody
see all
Images
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All lanes : Anti-GBP1 antibody [EPR8285] (ab131255) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : GBP1 knockout A549 cell lysate
Lane 3 : HeLa treated with 10ng/ml IFN-? for 24 hours, whole cell lysate
Lane 4 : Untreated HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab131255).
Lanes 1-4: Merged signal (red and green). Green - ab131255 observed at 68 kDa. Red - loading control ab8245 observed at 36 kDa.
ab131255 Anti-GBP1 antibody [EPR8285] was shown to specifically react with GBP1 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267202 (knockout cell lysate ab257960) was used. Wild-type and GBP1 knockout samples were subjected to SDS-PAGE. ab131255 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling GBP1 with ab131255 antibody at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131255).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131255).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab240050 has not yet been referenced specifically in any publications.