Recombinant Anti-GST antibody [EPR4236] (ab111947)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4236] to GST
- Suitable for: WB, ICC/IF, IP
- Reacts with: Species independent
Related conjugates and formulations
Overview
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Product name
Anti-GST antibody [EPR4236]
See all GST primary antibodies -
Description
Rabbit monoclonal [EPR4236] to GST -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- GST fusion proteins; Alpha-actinin-4-GST, AKT1/PKBa-GST or CDK7-GST.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4236 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
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ICC/IF |
1/100.
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IP |
1/20.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. |
ICC/IF
1/100. |
IP
1/20. |
Target
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Relevance
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. -
Alternative names
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
Images
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Immunofluorescence staining of 293T cells transfected with GST, with ab111947 at a working dilution of 1/100 and counter-stained with DAPI. Tubulin was also stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120). The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1/500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X-100. The negative controls are shown in the bottom hand panels - for the first negative control, anti-GST antibody (ab111947) was used at a dilution of 1/100 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/1000 and for the second negative control, mouse anti-tubulin (ab7291) and anti-rabbit secondary antibody (ab150077) were used.
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GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.
Lane 1: Primary: ab111947, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
Lane 2: Primary: ab111947, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate
Lane 3: Primary: ab172730, Rabbit monoclonal IgG
Sample: 293T transfected with GST tagged protein vector cell lysateAll lanes: Secondary: ab131366, Veriblot, 1/10000 dilution
Blocking and diluting buffer: 5% NFDM/TBST
Lanes 1-2 : Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution
Lane 3 : Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Lane 1 : Input - 293 transfected with GST tagged protein vector cell lysate at 10 µg
Lane 2 : (+) 293T transfected with GST tagged protein vector cell lysate
Lane 3 : (-) 293T transfected with GST tagged protein vector cell lysate
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution (VeriBlot)
Additional bands at: 41 kDa (possible tagged protein) -
Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution + GST recombinant protein at 0.01 µg
Predicted band size: 26 kDa -
(Cross IP) GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.
Lane 1: Primary: ab180123, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
Lane 2: Primary: ab180123, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate
Secondary: ab131366, Veriblot, 1/10000 dilution
Lane 3: Primary: ab172730, Rabbit monoclonal IgG
Sample: 293T transfected with GST tagged protein vector cell lysateAll lanes: Secondary: ab131366, Veriblot, 1/10000 dilution
Blocking and diluting buffer: 5% NFDM/TBST -
All lanes : Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution
Lane 1 : Alpha-actinin-4-GST fusion protein
Lane 2 : AKT1/PKBa-GST fusion protein
Lane 3 : CDK7-GST fusion protein
Lysates/proteins at 0.01 µg per lane.
Predicted band size: 26 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab111947 has been referenced in 19 publications.
- Yang G et al. The Effector Protein CgNLP1 of Colletotrichum gloeosporioides Affects Invasion and Disrupts Nuclear Localization of Necrosis-Induced Transcription Factor HbMYB8-Like to Suppress Plant Defense Signaling. Front Microbiol 13:911479 (2022). PubMed: 35770165
- Qiu Z et al. PLK1-mediated phosphorylation of PPIL2 regulates HR via CtIP. Front Cell Dev Biol 10:902403 (2022). PubMed: 36092721
- Chen L et al. Mangiferin prevents myocardial infarction-induced apoptosis and heart failure in mice by activating the Sirt1/FoxO3a pathway. J Cell Mol Med 25:2944-2955 (2021). PubMed: 33523605
- Xu Q et al. Aloin protects mice from diet-induced non-alcoholic steatohepatitis via activation of Nrf2/HO-1 signaling. Food Funct 12:696-705 (2021). PubMed: 33410857
- Xu Q et al. Adenosine 5'-monophosphate-activated protein kinase ameliorates bovine adipocyte oxidative stress by inducing antioxidant responses and autophagy. J Dairy Sci 104:4516-4528 (2021). PubMed: 33551161