Anti-TRPM8 antibody (ab3243)
Key features and details
- Rabbit polyclonal to TRPM8
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-TRPM8 antibody
See all TRPM8 primary antibodies -
Description
Rabbit polyclonal to TRPM8 -
Host species
Rabbit -
Specificity
Reacts with residues 278-292 and the C terminus sequence of the human trpm8 protein. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Synthetic peptide corresponding to Human TRPM8.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Immunogen affinity purified. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 127 kDa.
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IHC-P |
1/10 - 1/2000.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 127 kDa. |
IHC-P
1/10 - 1/2000. |
Target
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Function
Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. -
Tissue specificity
Expressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors. -
Sequence similarities
Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 79054 Human
- Omim: 606678 Human
- SwissProt: Q7Z2W7 Human
- Unigene: 366053 Human
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Alternative names
- Long transient receptor potential channel 6 antibody
- LTrpC-6 antibody
- LTrpC6 antibody
see all
Images
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Anti-TRPM8 antibody (ab3243) at 1/750 dilution + TRPM8 transfected Cos7 cell lysate
Predicted band size: 127 kDa -
ab3243 staining TRPM8 in human prostate cancer tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
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All lanes : Anti-TRPM8 antibody (ab3243) at 1000 cells
Lane 1 : Human liver tissue lysate
Lane 2 : Human hepatocyte lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit HRP at 1/1000 dilution
Predicted band size: 127 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (33)
ab3243 has been referenced in 33 publications.
- Liu JJ et al. Upregulation of TRPM8 can promote the colon cancer liver metastasis through mediating Akt/GSK-3 signal pathway. Biotechnol Appl Biochem 69:230-239 (2022). PubMed: 33432591
- Genovesi S et al. Trpm8 Expression in Human and Mouse Castration Resistant Prostate Adenocarcinoma Paves the Way for the Preclinical Development of TRPM8-Based Targeted Therapies. Biomolecules 12:N/A (2022). PubMed: 35204694
- Liu Y et al. AMTB, a TRPM8 antagonist, suppresses growth and metastasis of osteosarcoma through repressing the TGFβ signaling pathway. Cell Death Dis 13:288 (2022). PubMed: 35361751
- Zalles M et al. ELTD1 as a multi-focal target for malignant gliomas: preclinical studies. Neurooncol Adv 3:vdab132 (2021). PubMed: 34704036
- Gao Y et al. Saponins from Panax japonicus ameliorate age-related renal fibrosis by inhibition of inflammation mediated by NF-?B and TGF-ß1/Smad signaling and suppression of oxidative stress via activation of Nrf2-ARE signaling. J Ginseng Res 45:408-419 (2021). PubMed: 34025134