Name: Anti-Progesterone Receptor antibody [SP42]
Brand: RabMAb
SKU: ab101688
Rating: 5 (2 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP42] to Progesterone Receptor
Suitable for: Flow Cyt, ICC/IF, IHC-P
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

Product name

Anti-Progesterone Receptor antibody [SP42]
See all Progesterone Receptor primary antibodies
Description

Rabbit monoclonal [SP42] to Progesterone Receptor
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment within Human Progesterone Receptor aa 400-550. The exact sequence is proprietary.
Database link: P06401
Positive control
- IHC-P: Human breast cancer tissue, Human breast, Mouse breast and Rat breast tissues. Flow Cyt: T-47D cells ICC/IF: T-47D cells
General notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP42
Isotype

IgG
Research areas
- Neuroscience
- Development
- Neuroscience
- Processes

Alternative Versions
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human Progesterone Receptor protein (ab159105)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab101688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt		1/260.
ICC/IF		1/100.
IHC-P		1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate primary antibody for 30 minutes at room temperature.

Notes
Flow Cyt 1/260.
ICC/IF 1/100.
IHC-P 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate primary antibody for 30 minutes at room temperature.

Function

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
Sequence similarities

Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Domain

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Post-translational
modifications

Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
Cellular localization

Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
Target information above from: UniProt accession P06401 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 5241 Human
- Entrez Gene: 18667 Mouse
- Entrez Gene: 25154 Rat
- Omim: 607311 Human
- SwissProt: P06401 Human
- SwissProt: Q00175 Mouse
- SwissProt: Q63449 Rat
- Unigene: 32405 Human
- Unigene: 742403 Human
- Unigene: 12798 Mouse
- Unigene: 437703 Mouse
- Unigene: 10303 Rat
see all
Alternative names
- NR3C3 antibody
- Nuclear receptor subfamily 3 group C member 3 antibody
- PGR antibody
- PR antibody
- PRA antibody
- PRB antibody
- PRGR_HUMAN antibody
- Progesterone receptor antibody
- Progestin receptor form A antibody
- Progestin receptor form B antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on human breast cancer tissue, performed on a Leica Biosystems BOND^®RX instrument.
Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP42] (ab101688)

Immunocytochemistry/ Immunofluorescence analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab101688 at 1:100 (2.66 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat breast tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on rat breast tissue, performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse breast tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on mouse breast tissue, performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Sporadically nuclear staining on human breast tissue, performed on a Leica Biosystems BOND^® RX instrument.
Flow Cytometry - Anti-Progesterone Receptor antibody [SP42] (ab101688)

Flow Cytometry analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab101688 at 1:260 dilution (1.02 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution (0.61 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on human breast cancer, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101688 for 30 mins at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat breast tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution (0.61 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on rat breast, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101688 for 30 mins at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse breast tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution (0.61 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on mouse breast, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101688 for 30 mins at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution (0.61 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Sporadically nuclear staining on human breast, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101688 for 30 mins at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)

ab101688, at a 1/400 dilution, staining Progesterone Receptor in formalin fixed, paraffin embedded Human breast carcinoma by Immunohistochemistry.
Anti-Progesterone Receptor antibody [SP42] (ab101688)

Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

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Datasheet download

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Publishing research using ab101688? Please let us know so that we can cite the reference in this datasheet.

ab101688 has been referenced in 3 publications.

Lin YK et al. SCM-198 Prevents Endometriosis by Reversing Low Autophagy of Endometrial Stromal Cell via Balancing ERα and PR Signals. Front Endocrinol (Lausanne) 13:858176 (2022). PubMed: 35784569
Liu Y et al. Combining Organoid Models with Next-Generation Sequencing to Reveal Tumor Heterogeneity and Predict Therapeutic Response in Breast Cancer. J Oncol 2022:9390912 (2022). PubMed: 36046364
Chen P et al. Patient-Derived Organoids Can Guide Personalized-Therapies for Patients with Advanced Breast Cancer. Adv Sci (Weinh) 8:e2101176 (2021). PubMed: 34605222

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Mouse Tissue sections (Uterus)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Co-Detection Target retrieval (10x) (REF 323165)

Permeabilization

Yes - 1X - Tween20

Specification

Uterus

Blocking step

Co-Detection blocker (REF : 323170) as blocking agent for 15 minute(s)

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 03 2023

Application

Immunohistochemistry (Resin sections)

Sample

Rat Tissue sections (Mammary Gland)

Specification

Mammary Gland

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. MdImtiazul Kabir

Verified customer

Submitted May 19 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Purification notes

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Domain

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (3)

Customer reviews and Q&As

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Progesterone Receptor antibody [SP42]

Immunohistochemistry (Resin sections) abreview for Anti-Progesterone Receptor antibody [SP42]

Post-translational
modifications