Recombinant Anti-proNGF antibody [EP1320Y] (ab52918)
![Western blot - Anti-proNGF antibody [EP1320Y] (ab52918)](https://www.abcam.com/ps/products/52/ab52918/Images/ab52918-483789-anti-ngf-antibody-ep1320y-western-blot-mouse-rat-human-1.jpg "Western blot - Anti-proNGF antibody [EP1320Y] (ab52918)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1320Y] to proNGF
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-proNGF antibody [EP1320Y]
See all proNGF primary antibodies -
Description
Rabbit monoclonal [EP1320Y] to proNGF -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain, cerebellum, HeLa, MCF-7, and MDA-MB-231 cell lysates. Mouse brain, cerebellum, and thyroid cell lysates; Rat brain, cerebellum, and thyroid cell lysates. IHC-P: Human brain, human cerebral cortex. ICC/IF: U87-MG cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1320Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/300.
|
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| WB | (1) |
1/1000. Predicted molecular weight: 27 kDa.
For unpurified, use 1/500. |
| IHC-P | (3) |
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Notes |
|---|
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ICC/IF
1/300. |
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WB
1/1000. Predicted molecular weight: 27 kDa. For unpurified, use 1/500. |
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IHC-P
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). -
Involvement in disease
Neuropathy, hereditary sensory and autonomic, 5 -
Sequence similarities
Belongs to the NGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 4803 Human
- SwissProt: P01138 Human
- SwissProt: P01139 Mouse
- SwissProt: P25427 Rat
- Unigene: 2561 Human
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Alternative names
- Beta-nerve growth factor antibody
- Beta-NGF antibody
- NGF antibody
see all
Images
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Western blot - Anti-proNGF antibody [EP1320Y] (ab52918)All lanes : Anti-proNGF antibody [EP1320Y] (ab52918) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Human cerebellum lysate
Lane 4 : Mouse cerebellum lysate
Lane 5 : Rat cerebellum lysate
Lane 6 : Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 7 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 8 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsThe positive cell lines (Lanes 7-9) are consistent with the literature: PMID:12944907, Fig1.
Blocking buffer: 5% NFDM/TBST.
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![Western blot - Anti-proNGF antibody [EP1320Y] (ab52918)](https://www.abcam.com/ps/products/52/ab52918/Images/ab52918-243802-anti-ngf-antibody-ep1320y-western-blot.jpg)
Western blot - Anti-proNGF antibody [EP1320Y] (ab52918)All lanes : Anti-proNGF antibody [EP1320Y] (ab52918) at 1/1000 dilution (purified)
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal thymus lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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![Western blot - Anti-proNGF antibody [EP1320Y] (ab52918)](https://www.abcam.com/ps/products/52/ab52918/Images/ab52918-243804-anti-ngf-antibody-ep1320y-western-blot.jpg)
Western blot - Anti-proNGF antibody [EP1320Y] (ab52918)Anti-proNGF antibody [EP1320Y] (ab52918) at 1/1000 dilution (purified) + Mouse thyroid lysate at 20 µg
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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![Western blot - Anti-proNGF antibody [EP1320Y] (ab52918)](https://www.abcam.com/ps/products/52/ab52918/Images/ab52918-243803-anti-ngf-antibody-ep1320y-western-blot.jpg)
Western blot - Anti-proNGF antibody [EP1320Y] (ab52918)Anti-proNGF antibody [EP1320Y] (ab52918) at 1/2000 dilution (purified) + Rat thyroid lysate at 20 µg
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proNGF antibody [EP1320Y] (ab52918)](https://www.abcam.com/ps/products/52/ab52918/Images/ab52918-243823-anti-ngf-antibody-ep1320y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proNGF antibody [EP1320Y] (ab52918)Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab52918 at a working dilution of 1 in 250. The secondary antibody used is ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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![Immunocytochemistry/ Immunofluorescence - Anti-proNGF antibody [EP1320Y] (ab52918)](https://www.abcam.com/ps/products/52/ab52918/Images/ab52918-243830-anti-ngf-antibody-ep1320y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-proNGF antibody [EP1320Y] (ab52918)Immunofluorescence staining of U87-MG cells with purified ab52918 at a working dilution of 1 in 300, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab52918 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.
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![Western blot - Anti-proNGF antibody [EP1320Y] (ab52918)](https://www.abcam.com/ps/products/52/ab52918/Images/ab52918-65415-anti-ngf-antibody-ep1320y-western-blot.jpg)
Western blot - Anti-proNGF antibody [EP1320Y] (ab52918)Anti-proNGF antibody [EP1320Y] (ab52918) at 1/500 dilution (unpurified) + fetal thyroid tissue at 10 µg
Secondary
Goat anti rabbit IgG HRP antibody at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proNGF antibody [EP1320Y] (ab52918)](https://www.abcam.com/ps/products/52/ab52918/Images/ab52918-65414-anti-ngf-antibody-ep1320y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proNGF antibody [EP1320Y] (ab52918)Immunohistochemical staining of paraffin embedded human brain using unpurified ab52918 at 1/50-1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proNGF antibody [EP1320Y] (ab52918)](https://www.abcam.com/ps/products/52/ab52918/Images/ab52918-65413-anti-ngf-antibody-ep1320y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proNGF antibody [EP1320Y] (ab52918)This image is courtesy of an anonymous Abreview.Immunohistochemical staining of proNGF mouse tissue sections (formalin/ PFA-fixed paraffin-embedded tissue sections) with unpurified ab52918. The sections were formaldehyde fixed, subjected to heat mediated antigen retrieval and blocked for 10 minutes at 25°C. The primary antibody was diluted 1/50 and incubated with the sample for 1 hour at 25°C. An HRP polymer anti-rabbit IgG system was used undiluted, as the secondary antibody.
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![Anti-proNGF antibody [EP1320Y] (ab52918)](https://www.abcam.com/ps/products/52/ab52918/Images/ab52918-10-benefits-of-recombinant-antibodies.png)
Anti-proNGF antibody [EP1320Y] (ab52918)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (49)
ab52918 has been referenced in 49 publications.
- Chen WY et al. Nerve growth factor interacts with CHRM4 and promotes neuroendocrine differentiation of prostate cancer and castration resistance. Commun Biol 4:22 (2021). PubMed: 33398073
- Deng WW et al. Optogenetic Neuronal Stimulation Promotes Functional Recovery After Spinal Cord Injury. Front Neurosci 15:640255 (2021). PubMed: 33897353
- Zhou LT et al. Elevated Levels of miR-144-3p Induce Cholinergic Degeneration by Impairing the Maturation of NGF in Alzheimer's Disease. Front Cell Dev Biol 9:667412 (2021). PubMed: 33898468
- Zhang F et al. Maternal Subclinical Hypothyroidism in Rats Impairs Spatial Learning and Memory in Offspring by Disrupting Balance of the TrkA/p75NTR Signal Pathway. Mol Neurobiol N/A:N/A (2021). PubMed: 33966253
- Lin H et al. Nerve growth factor regulates liver cancer cell polarity and motility. Mol Med Rep 23:N/A (2021). PubMed: 33649819