Anti-Prostaglandin E Synthase/MPGES-1 antibody (ab62050)
Key features and details
- Rabbit polyclonal to Prostaglandin E Synthase/MPGES-1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Prostaglandin E Synthase/MPGES-1 antibody
See all Prostaglandin E Synthase/MPGES-1 primary antibodies -
Description
Rabbit polyclonal to Prostaglandin E Synthase/MPGES-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Monkey, Zebrafish
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use at an assay dependent concentration.
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| IHC-P | (1) |
Use a concentration of 10 µg/ml.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. |
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IHC-P
Use a concentration of 10 µg/ml. |
Target
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Function
Catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). -
Sequence similarities
Belongs to the MAPEG family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 417193 Chicken
- Entrez Gene: 282019 Cow
- Entrez Gene: 9536 Human
- Entrez Gene: 64292 Mouse
- Entrez Gene: 59103 Rat
- Entrez Gene: 544666 Zebrafish
- Omim: 605172 Human
- SwissProt: O14684 Human
see all -
Alternative names
- Glutathione S transferase 1 like 1 antibody
- MGC10317 antibody
- MGST IV antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin E Synthase/MPGES-1 antibody (ab62050)This image is courtesy of an anonymous Abreview
ab62050 staining Prostaglandin E Synthase/MPGES-1 in Mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton-X and blocked with 10% serum for 30 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) for 20 hours at 4°C. ab150075, a donkey anti-rabbit Alexa Fluor® 647- (1/200) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin E Synthase/MPGES-1 antibody (ab62050)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue, staining Prostaglandin E Synthase/MPGES-1 with ab62050 at 10 µg/ml.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab62050 has been referenced in 9 publications.
- Yamashita N et al. MUC1-C integrates activation of the IFN-? pathway with suppression of the tumor immune microenvironment in triple-negative breast cancer. J Immunother Cancer 9:N/A (2021). PubMed: 33495298
- Akthar I et al. In vitro and in vivo impairment of embryo implantation by commonly used fungicide Mancozeb. Biochem Biophys Res Commun 527:42-48 (2020). PubMed: 32446389
- Han L et al. Triterpenoid Saponins from Anemone flaccida Suppress Tumor Cell Proliferation by Regulating MAPK, PD1/PDL1, and STAT3 Signaling Pathways and Altering Cancer Metabolism. Onco Targets Ther 12:10917-10930 (2019). PubMed: 31849495
- Chen Z et al. CRISPR/Cas9-based liver-derived reporter cells for screening of mPGES-1 inhibitors. J Enzyme Inhib Med Chem 34:799-807 (2019). PubMed: 30879343
- Berisha B et al. Prostaglandins in Superovulation Induced Bovine Follicles During the Preovulatory Period and Early Corpus Luteum. Front Endocrinol (Lausanne) 10:467 (2019). PubMed: 31354631
- Berisha B et al. Changes in the expression of prostaglandin family members in bovine corpus luteum during the estrous cycle and pregnancy. Mol Reprod Dev 85:622-634 (2018). PubMed: 29877057
- Maseda D et al. mPGES-1-Mediated Production of PGE2 and EP4 Receptor Sensing Regulate T Cell Colonic Inflammation. Front Immunol 9:2954 (2018). PubMed: 30619314
- Uchida K et al. Transforming growth factor activating kinase 1 regulates extracellular matrix degrading enzymes and pain-related molecule expression following tumor necrosis factor-a stimulation of synovial cells: an in vitro study. BMC Musculoskelet Disord 18:283 (2017). PubMed: 28668088
- Shi X et al. The involvement and possible mechanism of NR4A1 in chondrocyte apoptosis during osteoarthritis. Am J Transl Res 9:746-754 (2017). PubMed: 28337303