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Anti-Prostate Specific Antigen antibody [8301] (ab403)

Reviews (1)Q&A (18)References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [8301] (ab403)

    Key features and details

    • Mouse monoclonal [8301] to Prostate Specific Antigen
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    Overview

    • Product name

      Anti-Prostate Specific Antigen antibody [8301]
      See all Prostate Specific Antigen primary antibodies

    • Description

      Mouse monoclonal [8301] to Prostate Specific Antigen

    • Host species

      Mouse

    • Specificity

      Human prostate-specific antigen - 100% PSA/alpha-1-ACT-complex - 59% Kallikrein - <0.04% PAP - <0.04% Cross-reactivity values have been calculated on weight/weight basis using 125-I-labelled human human prostate-specific antigen in assay. They can vary because of the presence of antigen or related impurities in protein preparations used for cross reactivity assays. Affinity constant: 1x1011 l/mol human prostate-specific antigen.

    • Tested applications

      Suitable for: IHC-Pmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      native protein (purified), corresponding to amino acids 158-163 of Human Prostate Specific Antigen

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab403 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Notes
    Application notes
    ELISA: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependent dilution.
    RIA: Use at an assay dependant concentration.
    Nanowires array: 1/50 - 1/500.
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Target

    • Function

      Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

    • Sequence similarities

      Belongs to the peptidase S1 family. Kallikrein subfamily.
      Contains 1 peptidase S1 domain.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • antigen, prostate-specific antibody
        • APS antibody
        • Gamma seminoprotein antibody
        • Gamma-seminoprotein antibody
        • hK3 antibody
        • Kallikrein 3 antibody
        • Kallikrein related peptidase 3 antibody
        • Kallikrein-3 antibody
        • KLK 3 antibody
        • KLK2A1 antibody
        • Klk3 antibody
        • KLK3_HUMAN antibody
        • P-30 antigen antibody
        • P30 antigen antibody
        • Prostate-specific antigen antibody
        • Psa antibody
        • Semenogelase antibody
        • Seminin antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [8301] (ab403)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [8301] (ab403)
        Human normal prostate. Staining is localised as cytoplasmic and secreted. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system, at room temperature: sections were rehydrated and antigen retrieved with buffers (citrate pH 6.1) in a PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

      References (5)

      Publishing research using ab403? Please let us know so that we can cite the reference in this datasheet.

      ab403 has been referenced in 5 publications.

      • Danthanarayana AN  et al. A multicolor multiplex lateral flow assay for high-sensitivity analyte detection using persistent luminescent nanophosphors. Anal Methods 12:272-280 (2020). PubMed: 32577135
      • Shu SK  et al. Phosphorylation and activation of androgen receptor by Aurora-A. J Biol Chem 285:33045-53 (2010). WB ; Human . PubMed: 20713353
      • Kim D  et al. Microarray-based multiplexed scanometric immunoassay for protein cancer markers using gold nanoparticle probes. Anal Chem 81:9183-7 (2009). PubMed: 19874062
      • Thaxton CS  et al. Nanoparticle-based bio-barcode assay redefines "undetectable" PSA and biochemical recurrence after radical prostatectomy. Proc Natl Acad Sci U S A 106:18437-42 (2009). Human . PubMed: 19841273
      • Zheng G  et al. Multiplexed electrical detection of cancer markers with nanowire sensor arrays. Nat Biotechnol 23:1294-301 (2005). PubMed: 16170313

      Customer reviews and Q&As

      Show All Reviews Q&A
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      11-19 of 19 Abreviews or Q&A

      Question

      The affinity constant for this product is listed as 1x10^11I/mol. What are the units on I?

      Read More
      Answer

      Thank you for your enquiry. My apologies for delay, it has taken some time to confirm this information for you. l stands for litre, and l/mol is for affinity: [AgAb]/[Ag][Ab], which gives units l/mol. I hope this is helpful. Should you require any further assistance, please do not hesitate to contact us again.

      Read More

      Question

      Thanks for the reply. Unfortunately we cannot order a large amount. Do you mind give me the contact information of the antibody originator? I thought it would not be very difficult by NOT adding BSA. Second question: if it is impossible to get the above BSA-free antibody, how about the ab403 and ab2218. Do they contain BSA or any other proteins in their storage buffer?

      Read More
      Answer

      Thank you for your email. I can't tell you the name of the originator of ab9537 as that information is proprietary. I double-checked ab403 and ab2218, and both of these do not contain BSA or other proteins in the storage buffer. Ab403 consists of preservative: 0.1% Sodium Azide, and constituents: sodium chloride, citrate / phosphate. pH 6.0. Ab2218 consists of preservative: 15mM sodium azide, and constituents: PBS. pH 7.4. Please contact us again if you have any additional questions or concerns.

      Read More

      Question

      Can you please find out for me if the immunogen was full-length PSA or PSA/alpha-1-ACT-complex? Can you give a better explanantion of how the cross-reactivity values were obtained? In other words, is the affinity constant for PSA/alpha-1-ACT-complex 59% of the following value for PSA: 1x1011 l/mol human prostate-specific antigen?

      Read More
      Answer

      Thank you for your enquiry and your patience. The preparation for the primary immunization was PSA/ACT-complex as in most cases the source for PSA is seminal fluid. Free PSA is mostly deriving from tumor tissue. The secondary (booster) immunization was peformed using highly purified PSA. The affinity value was determined using a standard RIA method. Cross-reactivity with PSA/ACT-complex was determined using RIA and a Scatchard's plot. Cross-reactivity of 59% means that the binding level is 59% compared to the binding to the PSA (that was used to determine the K-value). It should be kept in mind that cross-reactivities are very much dependent on the preparation, but also on the method. Hence they should not be taken as "absolute" values. You can find more information on these PSA clones from Tumor Biol. 20 (suppl. 1): 1-94 (1999). Wishing you a peaceful holiday and a prosperous and happy New Year!

      Read More

      Question

      I am looking for an anti-human PSA mAb for cytofluorimetry. Has any of your anti-PSA Abs ever been tested by this technique? Many thanks

      Read More
      Answer

      To our knowledge, none of our anti PSA antibodies have been tested in cytofluorimetry. However, ab403 is the most popular of all our anti PSA antibodies and works in many other applications so I'd recommend that you try this one.

      Read More

      Question

      thanks for the help. Unfortunately the information on the webpage and the datasheets are not sufficient. We need information about the antibody epitopes, since we would like to detect deletions of PSA. For this detection we need an antibody that detects an epitope between aa 18 and 60. Can you provide information about the epitopes of ab2218, ab403 and ab8681?

      Read More
      Answer

      Unfortunately, we do not have more information about this antibody (ab403). We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

      Read More

      Question

      Is this ab provided with a positive control lysate. If not, what kind of cell lysate do you test this ab with in your labs?

      Read More
      Answer

      No, this antibody is not supplied with a positive control cell lysate. I suggest that you take a look at reviews written by researchers using this antibody (please click on the reviews tab on the datasheet) where you will find suggestions on the use of this antibody on prostate tissue.

      Read More

      Question

      Is this a PSA antibody for the PSA-ACT complex, or to free PSA, or to another form of PSA?

      Read More
      Answer

      The antibody is to human prostate-specific antigen (hPSA) but also cross reacts with the PSA/alpha1-ACT complex

      Read More

      Question

      Can you tell me if your PSA antibody cross reacts with the mouse orthologue?

      Read More
      Answer

      To our knowledge, this antibody has not been tested in mouse tissue.

      Read More

      Question

      Do you have any further information how to use PSA1 to detect prostate specific antigen in western blot?

      Read More
      Answer

      This antibody can be used at 1ug/ml in Western blotting using the standard protocol in the protocols section of the website. For your particular application you may want o titrate it down to 0.1ug/ml if you see excessive background

      Read More

      11-19 of 19 Abreviews or Q&A

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