Recombinant Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1588Y] to Prostate Specific Antigen
- Suitable for: mIHC, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Prostate Specific Antigen antibody [EP1588Y]
See all Prostate Specific Antigen primary antibodies -
Description
Rabbit monoclonal [EP1588Y] to Prostate Specific Antigen -
Host species
Rabbit -
Specificity
The antibody provided good results in western blot when tested in human prostate lysate. Unfortunately, when 22RV1 cell lysate was used no band was detected and inconsistent results were obtained with LNCaP cell lysate (where different lots of cell lysate were used for the test). Please contact our Scientific Support if you have any feedback and/or questions. -
Tested applications
Suitable for: mIHC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Prostate Specific Antigen aa 200-300 (C terminal). The exact sequence is proprietary.
Database link: P07288 -
Positive control
- WB: LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate and LNCaP (Human prostate carcinoma epithelial cell) treated with 100nM DHT for 2 days whole cell lysate IHC-P: Human prostate cancer tissue. mIHC: Human prostate gland tissues.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 5.04 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1588Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
mIHC |
1/2000.
|
|
WB |
1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa).
For unpurified use at 1/10,000 - 1/50,000 |
|
IHC-P |
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250 |
Notes |
---|
mIHC
1/2000. |
WB
1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa). For unpurified use at 1/10,000 - 1/50,000 |
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250 |
Target
-
Function
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. -
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 354 Human
- Omim: 176820 Human
- SwissProt: P07288 Human
- Unigene: 171995 Human
-
Alternative names
- antigen, prostate-specific antibody
- APS antibody
- Gamma seminoprotein antibody
see all
Images
-
Fluorescence multiplex immunohistochemical analysis of human prostate gland tissue (formalin/PFA-fixed paraffin-embedded section). Panel A: merged staining of anti-Prostate Specific Antigen (ab76113, red; Opal™690), anti-Cytokeratin 5 (ab236216, green; Opal™520) and anti-Nkx3.1 (ab196020, gray; Opal™570) on human prostate gland tissue. Panel B: anti-Prostate Specific Antigen stained on cytoplasm of luminal cells. Panel C: anti-Cytokeratin 5 stained on basal cells. Panel D: anti-p63 stained on nucleus of luminal cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of ab76113 (1/2000), ab236216 (1/400) and ab196020 (1/2000) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
-
All lanes : Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113) at 1/1000 dilution ((Purified))
Lane 1 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4 : DU 145 (Human prostate carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?The expression profile observed is consistent with what has been described in the literatures (PMID: 24906821, 17620434 and 25076860).
-
All lanes : Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113) at 1/1000 dilution
Lane 1 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate
Lane 2 : LNCaP (Human prostate carcinoma epithelial cell) treated with 100nM DHT for 2 days whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate cancer tissue sections labeling Prostate Specific Antigen with purified ab76113 at 1/1000 dilution (0.51 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Fluorescence multiplex immunohistochemical analysis of human prostate gland tissue (formalin/PFA-fixed paraffin-embedded section). Panel A: merged staining of anti-p63 (ab124762, magenta; Opal™690), anti-Cytokeratin 5 (ab236216, green; Opal™520) and anti-Prostate Specific Antigen (ab76113, red; Opal™570) on human prostate gland tissue. Panel B: anti-Prostate Specific Antigen stained on luminal cells. Panel C: anti-Cytokeratin 5 stained on cytoplasm of basal cells. Panel D: anti-p63 stained on nucleus of basal cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of ab124762 (1/5000), ab236216 (1/400), and ab76113 (1/2000) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
-
Fluorescence multiplex immunohistochemical analysis of human prostate gland tissue (formalin/PFA-fixed paraffin-embedded section). Panel A: merged staining of anti-Collagen VI (ab271938, magenta; Opal™690), anti-Cytokeratin 5 (ab236216, green; Opal™520) and anti-Prostate Specific Antigen (ab76113, red; Opal™570) on human prostate gland tissue. Panel B: anti-Prostate Specific Antigen stained on luminal cells. Panel C: anti-Cytokeratin 5 stained on basal cells. Panel D: anti-Collagen VI stained on stroma. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of ab271938 (1/500), ab236216 (1/400), and ab76113 (1/2000) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (11)
ab76113 has been referenced in 11 publications.
- Corbin JM et al. Seed-mediated RNA interference of androgen signaling and survival networks induces cell death in prostate cancer cells. Mol Ther Nucleic Acids 24:337-351 (2021). PubMed: 33850637
- Liu X et al. Human follicular fluid proteome reveals association between overweight status and oocyte maturation abnormality. Clin Proteomics 17:22 (2020). PubMed: 32528235
- Ci X et al. Conditionally Reprogrammed Cells from Patient-Derived Xenograft to Model Neuroendocrine Prostate Cancer Development. Cells 9:N/A (2020). PubMed: 32512818
- Li F et al. XPNPEP2 is associated with lymph node metastasis in prostate cancer patients. Sci Rep 9:10078 (2019). PubMed: 31296901
- Yao J et al. Bioconjugated, Single-Use Biosensor for the Detection of Biomarkers of Prostate Cancer. ACS Omega 3:6411-6418 (2018). PubMed: 30023946