Anti-Prostate Specific Antigen antibody [SP317] (ab224799)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [SP317] (ab224799)](https://www.abcam.com/ps/products/224/ab224799/Images/ab224799-3-AntiProstate-Specific-Antigen-antibody-SP317-IHC-human-prostate.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [SP317] (ab224799)")
Key features and details
- Rabbit monoclonal [SP317] to Prostate Specific Antigen
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Prostate Specific Antigen antibody [SP317]
See all Prostate Specific Antigen primary antibodies -
Description
Rabbit monoclonal [SP317] to Prostate Specific Antigen -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Prostate Specific Antigen (internal sequence). The exact sequence is proprietary.
Database link: P07288 -
Positive control
- WB: LNCaP cell lysate. IHC-P: Human prostate tissue.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP317 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/100. Predicted molecular weight: 29 kDa.
|
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| IHC-P |
1/100.
Perform heat mediated antigen retrieval with EDTA pH 8.0 buffer before commencing with IHC staining protocol. |
| Notes |
|---|
|
WB
1/100. Predicted molecular weight: 29 kDa. |
|
IHC-P
1/100. Perform heat mediated antigen retrieval with EDTA pH 8.0 buffer before commencing with IHC staining protocol. |
Target
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Function
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. -
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 354 Human
- Omim: 176820 Human
- SwissProt: P07288 Human
- Unigene: 171995 Human
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Alternative names
- antigen, prostate-specific antibody
- APS antibody
- Gamma seminoprotein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [SP317] (ab224799)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate tissue sections labeling Prostate Specific Antigen with ab224799 at 1/100 dilution (3.83 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on the human prostate, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224799 for 10 mins at room temperature. -
![Western blot - Anti-Prostate Specific Antigen antibody [SP317] (ab224799)](https://www.abcam.com/ps/products/224/ab224799/Images/ab224799-286008-anti-prostate-specific-antigen-antibody-sp317-western-blot.jpg)
Western blot - Anti-Prostate Specific Antigen antibody [SP317] (ab224799)Anti-Prostate Specific Antigen antibody [SP317] (ab224799) at 1/100 dilution + LNCaP (human prostate cancer cell line) cell lysate
Predicted band size: 29 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [SP317] (ab224799)](https://www.abcam.com/ps/products/224/ab224799/Images/ab224799-286009-anti-prostate-specific-antigen-antibody-sp317-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [SP317] (ab224799)Formalin-fixed, paraffin-embedded human prostate tissue stained for Prostate Specific Antigen using ab224799 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab224799 has been referenced in 1 publication.
- Cheaito K et al. Establishment and characterization of prostate organoids from treatment-naïve patients with prostate cancer. Oncol Lett 23:6 (2022). PubMed: 34820005