Anti-Proteasome 19S S4/PSMC1 antibody (ab3317)
Key features and details
- Rabbit polyclonal to Proteasome 19S S4/PSMC1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Proteasome 19S S4/PSMC1 antibody
See all Proteasome 19S S4/PSMC1 primary antibodies -
Description
Rabbit polyclonal to Proteasome 19S S4/PSMC1 -
Host species
Rabbit -
Specificity
Detects proteasome 19S subunit S4. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Proteasome 19S S4/PSMC1. Yeast proteasome 19S subunit S4.
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Positive control
- WB: HeLa cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 49 kDa).
This antibody detects a 56 kDa protein representing proteasome 19S subunit S4 in HeLa cell lysate. This antibody detects, to a lesser extent, an ~35 kDa protein which could correspond to subunit degradation product. |
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 49 kDa). This antibody detects a 56 kDa protein representing proteasome 19S subunit S4 in HeLa cell lysate. This antibody detects, to a lesser extent, an ~35 kDa protein which could correspond to subunit degradation product. |
Target
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Function
The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. -
Sequence similarities
Belongs to the AAA ATPase family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5700 Human
- Omim: 602706 Human
- SwissProt: P62191 Human
- Unigene: 356654 Human
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Alternative names
- 26S protease regulatory subunit 4 antibody
- 26S proteasome AAA-ATPase subunit RPT2 antibody
- MGC24583 antibody
see all
Images
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Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (ab3317)
Western blot of proteasome 19S subunit S4 on HeLa cell lysate using ab3317.
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab3317 has been referenced in 4 publications.
- Adelöf J et al. PA28aß overexpression enhances learning and memory of female mice without inducing 20S proteasome activity. BMC Neurosci 19:70 (2018). PubMed: 30400847
- Zhou X et al. HIV Replication in CD4+ T Lymphocytes in the Presence and Absence of Follicular Dendritic Cells: Inhibition of Replication Mediated by {alpha}-1-Antitrypsin through Altered I{kappa}B{alpha} Ubiquitination. J Immunol 186:3148-55 (2011). WB, IP . PubMed: 21263074
- Rousseau E et al. Misfolding of proteins with a polyglutamine expansion is facilitated by proteasomal chaperones. J Biol Chem 284:1917-29 (2009). WB, ICC/IF ; Human, Rat . PubMed: 18986984
- Sun X et al. Molecular characterization of tumour heterogeneity and malignant mesothelioma cell differentiation by gene profiling. J Pathol 207:91-101 (2005). PubMed: 16007577
