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Anti-Protein A antibody (ab19483)

Submit a review Q&A (11)References (1)

Key features and details

  • Chicken polyclonal to Protein A
  • Suitable for: Sandwich ELISA, WB
  • Reacts with: Staphylococcus aureus
  • Isotype: IgY

Overview

  • Product name

    Anti-Protein A antibody
    See all Protein A primary antibodies

  • Description

    Chicken polyclonal to Protein A

  • Host species

    Chicken

  • Tested applications

    Suitable for: Sandwich ELISA, WBmore details

  • Species reactivity

    Reacts with: Staphylococcus aureus

  • Immunogen

    Recombinant full length protein corresponding to Staphylococcus aureus Protein A.
    Database link: P02976

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgY

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab19483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
Use at an assay dependent concentration.

ab19483 can work in direct ELISA, but not indirect ELISA (because any secondary antibody would bind to protein A directly as well as the primary antibody, thus leading to high background – due to the nature of protein A). ab18596 as detection antibody for sELISA.
WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).

50 kDa band is due to cleavage of the signal peptide and propeptide
Notes
Sandwich ELISA
Use at an assay dependent concentration.

ab19483 can work in direct ELISA, but not indirect ELISA (because any secondary antibody would bind to protein A directly as well as the primary antibody, thus leading to high background – due to the nature of protein A). ab18596 as detection antibody for sELISA.
WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).

50 kDa band is due to cleavage of the signal peptide and propeptide

Target

  • Relevance

    Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.

  • Cellular localization

    Secreted; cell wall.

  • Database links

    • Alternative names

      • gG-binding protein A antibody
      • Immunoglobulin G binding protein A antibody
      • SPA antibody
      • Staphylococcal protein A antibody

    References (1)

    Publishing research using ab19483? Please let us know so that we can cite the reference in this datasheet.

    ab19483 has been referenced in 1 publication.

    • Seiler BT  et al. Broad-spectrum capture of clinical pathogens using engineered Fc-mannose-binding lectin enhanced by antibiotic treatment. F1000Res 8:108 (2019). PubMed: 31275563

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 11 Abreviews or Q&A

    Question

    Could you please send me expiry dating and data on the following products/lots? Chicken polyclonal antibody to Protein A (1 mg/mL from Abcam Cat. # ab19483) Lot: GR58389-4 Chicken polyclonal antibody to Protein A (HRP) (1 mg/mL from Abcam Cat. # ab18596) Lot: GR105326-2

    Read More
    Answer

    We do not have specific use by dates for our antibodies. If stored correctly, antibodies remain suitable for use for months and even for years. Please read our instructions on how to store antibodies on our website (https://www.abcam.com/index.html?pageconfig=popular_protocols). However, we do suggest the following approximate use by dates for all products based from timne of delivery: room temperature: 1 week + 4°C: 1 year - 20°C: 5 years - 80°C: 10 years These products are covered by our Abpromise guarantee fro a period of one year. We are happy provide scientific support at any time. If you are using the product in species and applications listed on the datasheet and contact us within twelve months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue.

    Read More

    Question

    Dear Madam or Sir, we would be interested in knowing how many vials of the product no 19483, lots GR58389 - X and prod no 18596 lots GR74278 - X are still available. Is it correct that the last number of the lot (indicated here as X) indicates the filling date and that the batches filled on different days should be comparable? I am looking forward to you reply.

    Read More
    Answer

    Thank you for contacting us.

    Yes, you are right. The number after the dash signifies when the lot was aliquoted. If the number directly after the "GR" is the same then it is the same lot.

    For ab19483, chicken polyclonal to Protein A, we have 16 vials of lot GR58389-4 in our US office ready to ship and an additional 30 units of lots GR58389-3 and GR58389-4 in our UK office.

    For ab18596, chicken polyclonal to Protein A (HRP), we have 1 vial of GR74278-4 in our UK office which would take a few extra days to ship to you. We have two new lots in for that antibody, but only 1 vial left of the lot you requested.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Inquiry: Dear Madam or Sir, we would be interested in knowing how many vials of the product no 19483, lots GR58389 - X and prod no 18596 lots GR74278 - X are still available. Is it correct that the last number of the lot (indicated here as X) indicates the filling date and that the batches filled on different days should be comparable? I am looking forward to you reply.

    Read More
    Answer

    Thank you for contacting us.

    We currently have x vials of ab19483, lot GR58389 worldwide (x in UK office, x in US office, x in Japan office.)

    For ab18596, we only have 1 vial of lot GR74278 in stock, however we do hold 8 other vials of different stocks of this antibody in the UK.

    The way we number our lots is indeed the way you mentioned. The first part of the lot number e.g GR12345 corresponds to one particular lot. The last number e.g GR12345-X corresponds to the a particular date where we receieved the antibody from our labs. This means that lots GR12345-1 and GR12345-2 of a particularly antibody originate from the same stock but were delivered to our stock rooms at different days therefore these should be identical.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Inquiry: I want to know if protA EIAs from a specific lot number are still available, Chk pAb anti-Protein A, ab19483, GR58389-2 AND Chk pAb anti-Protein A - HRP, ab18596, GR74278-2.

    Read More
    Answer

    Thank you for your enquiry and your interest in our products.

    I have checked the stock viability and can confirm the followings:

    - ab19483 - several vials from the same bleed and purification;

    - ab18596 - only one vial left from the batch required

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Question

    Hi



    Sorry – the antibody I am interested in is ab19483. I noticed that you have previously emailed ELISA data out, and I’d also be interested to see this data.



    Thanks for your help

    Read More
    Answer

    Thank you for your inquiry.

    I attached the ELISA testing data of ab19483 to this email for your information and wish you good lcuk with your research.

    Read More

    Question

    Would like to see a graph of ELISA data with this antibody.

    Read More
    Answer

    Thank you very much for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

    I have attached the ELISA data and graph for ab19483.

    I hope that this information will be useful, but if there is anything else that you need from us please let me know and I'll be happy to help.

    Read More

    Question

    Would like to see a graph of ELISA data with this antibody.

    Read More
    Answer

    Thank you very much for your call last week and for your patience while I've been in contact with the lab regarding your enquiry.

    My colleagues are gathering this data and we will send it to you as soon as possible. I apologize for the delay, but please let me know if there is anything else that we can do for you in the meantime.

    Read More

    Question

    Currently we are working with two of your antibodies: Chicken polyclonal to Protein A (ab19483) and Anti-Protein A antibody-HRP (ab18596). On your product sheet I found that the Chicken polyclonal to Protein A is IgY an the Anti-Protein A antibody-HRP is IgG. However, both anti-bodies are derived from chicken, so I was thinking they should both be IgY?

    Looking on the internet I found that chicken IgG and chicken IgY are two different names for the same antibody, which are used interchangeably. However, for me it was a bit confusing. Therefore, I was wondering whether you can confirm that both these anti-bodies (ab19483 and ab18596) are the same isotype?

    Are they actually the same anti-body with and without HRP-conjugate or is there a reason why one of them is called IgG and the other IgY?

    Thanks in advance,

    Read More
    Answer

    Thank you for contacting us. I apologize for being late in getting back to you.

    I can confirm that both antibodies ab18596 and ab19483 have similar IgY isotype.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Can I please obtain COAs for these two products:

    Ab18598 Chicken pAb to Protein A (Biotin) Lot# - GR58388
    Ab19483 Chicken pAb to Protein A Lot# - GR41744

    Thank you!

    Read More
    Answer

    Thank you for contacting Abcam regarding ab18598 and ab19483.


    Attached please find the CoC documents you requested.


    I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

    Read More

    Question

    I just purchased 3 of your chicken anti-Protein A antibodies, ab18596 (HRP-labeled), ab18598 (Biotin-labeled), and ab19483 (unconjugated). We were wondering, are these all the same antibody, or are the labeled antibodies different that the unconjugated one?

    Read More
    Answer

    Thank you for contacting us.

    The conjugated antibodies ab18596 and ab18598 are prepared from the unconjugated ab19483. The formulations differ (for instance, there is no azide in the HRP conjugate buffer) but the antibody is the same for all three.

    I fixed a mistake on the ab19483 datasheet regarding the purity, which was inconsistent with the other two datasheets. It is immunogen-affinity-purified.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    1-10 of 11 Abreviews or Q&A

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