Recombinant Anti-PSD93 antibody [EPR26468-112] (ab289972)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD93 antibody [EPR26468-112] (ab289972)](https://www.abcam.com/ps/products/289/ab289972/Images/ab289972-7-anti-psd93-antibody-epr26468112-immunohistochemistry-human-cerebrum.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD93 antibody [EPR26468-112] (ab289972)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26468-112] to PSD93
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PSD93 antibody [EPR26468-112]
See all PSD93 primary antibodies -
Description
Rabbit monoclonal [EPR26468-112] to PSD93 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details
Unsuitable for: Flow Cyt (Intra) or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human cerebellum tissue lysate; Mouse brain tissue lysate; Rat brain tissue lysate. IHC-P: Mouse cerebrum tissue; Rat cerebrum tissue; Human cerebrum tissue. IP: SH-SY5Y and Neuro-2a whole cell lysates.
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General notes
DLG2 is an alternative name for PSD93.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26468-112 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab289972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| IP |
1/30.
|
|
| WB |
1/1000. Detects a band of approximately 25-110 kDa (predicted molecular weight: 97 kDa).
|
| Notes |
|---|
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/30. |
|
WB
1/1000. Detects a band of approximately 25-110 kDa (predicted molecular weight: 97 kDa). |
Target
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Function
Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses. -
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. -
Domain
An N-terminally truncated L27 domain is predicted in isoform 2 at positions 1 through 27. -
Post-translational
modificationsPalmitoylation of isoform 1 is not required for targeting to postsynaptic density. -
Cellular localization
Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Membrane. Cell projection, axon. Concentrated in soma and postsynaptic density of a subset of neurons. - Information by UniProt
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Database links
- Entrez Gene: 1740 Human
- Entrez Gene: 23859 Mouse
- Entrez Gene: 64053 Rat
- Omim: 603583 Human
- SwissProt: Q15700 Human
- SwissProt: Q91XM9 Mouse
- SwissProt: Q63622 Rat
- Unigene: 367656 Human
see all -
Alternative names
- Channel associated protein of synapse 110 antibody
- Channel associated protein of synapses 110kD antibody
- Channel-associated protein of synapse-110 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD93 antibody [EPR26468-112] (ab289972)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling PSD93 with ab289972 at 1/500 followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with ab289972 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS instead of primary antibody, followed by LeicaDS9800 (Bond™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunoprecipitation - Anti-PSD93 antibody [EPR26468-112] (ab289972)](https://www.abcam.com/ps/products/289/ab289972/Images/ab289972-10-anti-psd93-antibody-epr26468112-immunoprecipitation-neuro2a.jpg)
Immunoprecipitation - Anti-PSD93 antibody [EPR26468-112] (ab289972)PSD93 was immunoprecipitated from Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with ab289972 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289972 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug
Lane 2: ab289972 IP in Neuro-2a whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab289972 in Neuro-2a whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
-
![Western blot - Anti-PSD93 antibody [EPR26468-112] (ab289972)](https://www.abcam.com/ps/products/289/ab289972/Images/ab289972-483796-anti-psd93-antibody-epr26468112-western-blot-lysate1.jpg)
Western blot - Anti-PSD93 antibody [EPR26468-112] (ab289972)All lanes : Anti-PSD93 antibody [EPR26468-112] (ab289972) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 97 kDa
Observed band size: 25-110 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
DLG2 has many splicing forms. The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 14724236).
Negative control: spleen (PMID: 7590743)
Exposure time: 15 seconds
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD93 antibody [EPR26468-112] (ab289972)](https://www.abcam.com/ps/products/289/ab289972/Images/ab289972-6-anti-psd93-antibody-epr26468112-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD93 antibody [EPR26468-112] (ab289972)Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling PSD93 with ab289972 at 1/500 followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab289972 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: PBS instead of primary antibody, followed by LeicaDS9800 (Bond™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunoprecipitation - Anti-PSD93 antibody [EPR26468-112] (ab289972)](https://www.abcam.com/ps/products/289/ab289972/Images/ab289972-9-anti-psd93-antibody-epr26468112-immunoprecipitation-shsy5y.jpg)
Immunoprecipitation - Anti-PSD93 antibody [EPR26468-112] (ab289972)PSD93 was immunoprecipitated from SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with ab289972 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289972 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 10 ug
Lane 2: ab289972 IP in SH-SY5Y whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab289972 in SH-SY5Y whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD93 antibody [EPR26468-112] (ab289972)](https://www.abcam.com/ps/products/289/ab289972/Images/ab289972-5-anti-psd93-antibody-epr26468112-immunohistochemistry-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD93 antibody [EPR26468-112] (ab289972)Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling PSD93 with ab289972 at 1/500 followed by LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on mouse cerebrum. The section was incubated with ab289972 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Western blot - Anti-PSD93 antibody [EPR26468-112] (ab289972)](https://www.abcam.com/ps/products/289/ab289972/Images/ab289972-483797-anti-psd93-antibody-epr26468112-western-blot-recombinant.jpg)
Western blot - Anti-PSD93 antibody [EPR26468-112] (ab289972)All lanes : Anti-PSD93 antibody [EPR26468-112] (ab289972) at 1/1000 dilution
Lane 1 : His-tagged Human DLG2 recombinant protein (20ng)
Lane 2 : His-tagged Human DLG1 recombinant protein (20ng)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 97 kDa
Observed band size: 25-110 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with human DLG1.
Both recombinant proteins were made in-house and expressed from E.coli expression systems.
Exposure time: 15 seconds
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD93 antibody [EPR26468-112] (ab289972)](https://www.abcam.com/ps/products/289/ab289972/Images/ab289972-8-anti-psd93-antibody-epr26468112-immunohistochemistry-human-cardiac-muscle.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD93 antibody [EPR26468-112] (ab289972)Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling PSD93 with ab289972 at 1/500 (1.222 followed by LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Negative control: No staining on human cardiac muscle. The section was incubated with ab289972 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin..
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Western blot - Anti-PSD93 antibody [EPR26468-112] (ab289972)](https://www.abcam.com/ps/products/289/ab289972/Images/ab289972-483795-anti-psd93-antibody-epr26468112-western-blot-lysate.jpg)
Western blot - Anti-PSD93 antibody [EPR26468-112] (ab289972)Anti-PSD93 antibody [EPR26468-112] (ab289972) at 1/1000 dilution + Human cerebellum tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 97 kDa
Observed band size: 25-110 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
DLG2 has many splicing forms. The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 14724236).
Exposure time: 5.5 seconds
-
![Anti-PSD93 antibody [EPR26468-112] (ab289972)](https://www.abcam.com/ps/products/289/ab289972/Images/ab289972-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-PSD93 antibody [EPR26468-112] (ab289972)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab289972 has not yet been referenced specifically in any publications.