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Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)

Reviews (3)Q&A (4)References (47)
Immunocytochemistry/ Immunofluorescence - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)
  • Western blot - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)
  • Flow Cytometry - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)
  • Immunocytochemistry/ Immunofluorescence - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)
  • Western blot - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)

Key features and details

  • Mouse monoclonal [7E3-1B8] to PSD95 - Synaptic Marker
  • Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker
    See all PSD95 primary antibodies

  • Description

    Mouse monoclonal [7E3-1B8] to PSD95 - Synaptic Marker

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human

  • Immunogen

    Recombinant full length protein corresponding to Rat PSD95.

  • Positive control

    • ICC/IF: Rat primary hippocampal neurons; HaCaT cells; mouse backskin tissue section. WB: Rat brain membrane lysate; bovine brain tissue extract. Flow Cyt: K562 cells. IHC-P: Mouse backskin tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab13552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa.
ICC/IF (1)
Use at an assay dependent concentration.
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt
Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa.
ICC/IF
Use at an assay dependent concentration.
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt
Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.

  • Tissue specificity

    Brain.

  • Sequence similarities

    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.

  • Domain

    The PDZ domain 3 mediates interaction with ADR1B.
    The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.

  • Post-translational
    modifications

    Palmitoylation of isoform 1 is required for targeting to postsynaptic density.

  • Cellular localization

    Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Discs large homolog 4 antibody
    • Disks large homolog 4 antibody
    • DLG 4 antibody
    • Dlg4 antibody
    • DLG4_HUMAN antibody
    • FLJ97752 antibody
    • FLJ98574 antibody
    • Human post synaptic density protein 95 antibody
    • Post synaptic density protein 95 antibody
    • Postsynaptic density protein 95 antibody
    • PSD 95 antibody
    • PSD-95 antibody
    • PSD95 antibody
    • SAP 90 antibody
    • SAP-90 antibody
    • SAP90 antibody
    • Synapse associated protein 90 antibody
    • Synapse-associated protein 90 antibody
    • Tax interaction protein 15 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)
    Immunocytochemistry/ Immunofluorescence - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)This image is courtesy of an anonymous Abreview

    Central Panel: ab13552 staining PSD95 in rat primary hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS-BSA 3%) for 30 minutes at 20°C. An Cy5®-conjugated Donkey polyclonal to mouse IgG (dilution 1/500) was used as secondary antibody.
    Left-hand panel: Homer GFP.
    Right-hand panel: Merge.

    See Abreview

  • Western blot - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)
    Western blot - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)
    Western blot analysis of bovine brain tissue extract, probed with ab13552. Western blot analysis of bovine brain tissue extract, probed with ab13552.
  • Flow Cytometry - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)
    Flow Cytometry - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)
    Overlay histogram showing K562 cells stained with ab13552 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13552, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)

    ab13552 at 1/100 dilution staining PSD95 in mouse backskin tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. The image show basal, hair follicle and dermal staining.

  • Immunocytochemistry/ Immunofluorescence - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)
    Immunocytochemistry/ Immunofluorescence - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)
    Immunocytochemistry/ Immunofluorescence analysis of HaCaT cells labeling PSD95 with ab13552 at 1/100 dilution. Cells were fixed with 00% cold methanol for 10 minutes at -20°C. A FITC Goat Anti-Mouse antibody at 1/50 was used as secondary. The image shows Filamentouslike staining. 
  • Western blot - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)
    Western blot - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)
    Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552) at 1/1000 dilution + Rat brain membrane lysate.

References (47)

Publishing research using ab13552? Please let us know so that we can cite the reference in this datasheet.

ab13552 has been referenced in 47 publications.

  • Fu Q  et al. P-Rex2 mediation of synaptic plasticity contributes to bone cancer pain. Mol Pain 18:17448069221076460 (2022). PubMed: 35083941
  • Milky B  et al. Long-term adherence of human brain cells in vitro is enhanced by charged amine-based plasma polymer coatings. Stem Cell Reports 17:489-506 (2022). PubMed: 35180396
  • Huang Q  et al. Inhibition of ERK1/2 regulates cognitive function by decreasing expression levels of PSD-95 in the hippocampus of CIH rats. Eur J Neurosci 55:1471-1482 (2022). PubMed: 35243702
  • Marcatti M  et al. Aβ/tau oligomer interplay at human synapses supports shifting therapeutic targets for Alzheimer's disease. Cell Mol Life Sci 79:222 (2022). PubMed: 35377002
  • Lu Q  et al. Selective activation of ABCA1/ApoA1 signaling in the V1 by magnetoelectric stimulation ameliorates depression via regulation of synaptic plasticity. iScience 25:104201 (2022). PubMed: 35479414
View all Publications for this product

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker

 Average
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Cynomolgus monkey Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
Brain
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 31 2018

Immunohistochemistry free floating abreview for Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker

 Good
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 30 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary hippocampal neurons)
Permeabilization
Yes - Triton 0.3%
Specification
Primary hippocampal neurons
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 11 2010

Question

I have been doing a lot of immunocytochemistry with this antibody. Previously it has been working perfect, but since a few months with a new lot get a lot of unspecific staining. I've tried different blocking techniques, different permeabilizations and at least 3 different secondary antibodies, so I'm left with the conclusion that there is something that is not working for me with this new lot. Other antibodies that I'm using are working well.

Send me the questionaire and I will fill it out. Maybe you could send me the alternative PSD-95 mouse antibody Anti-PSD95 antibody [6G6-1C9] (ab2723), so that I could try that one instead, since there are no other lots of the one that I have? We have bought 2 of ab13552 Lot GR42550-5, the first one in june 2012 and the second one only a few weeks ago, and they give the same results.

Thank you!

Read More
Answer

Thank you for your reply.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Before deciding how to proceed, I would like to investigate this particular case further for you, and also obtain some further information for our quality records.

In order to do this, I have enclosed a questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily. I have completed the sections for which information has already been provided.

In the event that we cannot get the antibody functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. We are happy to provide an alternative if requested if we are not able to get the antibody working for you.

Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.

Order Details

Antibody code:

Lot number:

Purchase order number
or preferably Abcam order number:


General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, low signal, non-specific satining etc.)

Sample (Species/Tissue/Cell Type/Cell Line etc.)


Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)


Antigen retrieval (Enzymatic method, Heat mediated technique etc.)


Permeabilization step


Blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method


Positive and negative controls used (please specify)


Optimization attempts (problem solving)
How many times have you tried the IHC?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No


What steps have you altered?


Additional Notes


We would appreciate if you are also able to provide any image which woudl help us to assess the results. From the lot that has worked, and the lot that has not.

Read More

Question

ab13552 Lot GR42550-5 doesn't work as well as a previous lot I've brought
Are there any suggestions why this might be?
Could you let me know whether there have been other complaints about this lot?

Read More
Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this lot of antibody.

In answer to your question, I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch.

I would also like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB, ICC/IF, IHC-P, Flow Cyt and in use, Rat, Cow and Human samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I would like to send you a technical questionnaire. This will help you put the information we require together very easily. I would appreciate if you could confirm which application you have been using, so I can send the correct questionnaire.

I would appreciate if you are able to provide any image which would help us to assess the results.

Thank you for your time and cooperation. We look forward to hearing from you with the requested details.

Read More

Question

Thank you very much for all this information. I would be interested by the first one, the goat PSD-95 which gives nice data.I think it should work and I also have free floating material available so this would be the best option for me.In advance thank you very much for the replacement, for your help and all the information.

Read More
Answer

Thank you for confirming these details and for your cooperation.

I am sorry ab13552 did not perform well enough for your experiments and for the inconvenience this has caused. But I am happy that we have an alternative available that hopefully works for you. As requested, I have issued a free of charge replacement with ab12093 with the order number x.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Question

Thank you very much for your quick reply. I was wondering whether you would have an PSD-95 antibody that has been tested for IHC-FoFr but raised against mouse (or other species different from rabbit ?) , cause the one you suggest is raised against rabbit and we plan to do some colocalization study with a VGlut antibody (working fine).

Read More
Answer

Thank you for your answer.

Unfortunately, all the PSD-95 antibodies that we can guarantee for IHC-FoFr are rabbit polyclonals (ab16493, ab16495 and ab18258).

Having said this, we have goat polyclonal anti-PSD-95 antibody ab12093 available which has been tested for IHC on paraffin-embedded sections (IHC-P) and is working for free-floating sections (IHC-FrFl) - details about the IHC-FrFl protocol can be found in the publication by Koffie et al (PMID:19228947, attached). These applications may be closer to your experimental settings and thus this antibody might work for you. If you are happy to try it with this one, I would be pleased to arrange the replacement for you.
https://www.abcam.com/PSD95-antibody-ab12093.html https://www.abcam.com/PSD95-antibody-ab12093.html.

Also, we have the mouse monoclonal antibody ab99009 available which is guaranteed to work on paraffin-embedded sections as well as on frozen sections (IHC-P and -Fr), and thus there is a chance that it might work for your experimental layout. If you prefer this one, I could send it to you.
https://www.abcam.com/PSD95-antibody-S28-43-ab99009.html https://www.abcam.com/PSD95-antibody-S28-43-ab99009.html.

However, I do not want to talk you into using these antibodies, and if none of the above is an option for you, I would be pleased to send either any other primary antibody of your choice or issue a credit note.

I look forward to hearing from you with details of how you would like to proceed.

Read More

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