Recombinant Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
![Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)](https://www.abcam.com/ps/products/177/ab177159/Images/ab177159-397640-anti-psip1ledgf-antibody-epr11890-western-blot-human-knockout-lysate.jpg "Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11890] to PSIP1/LEDGF
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PSIP1/LEDGF antibody [EPR11890]
See all PSIP1/LEDGF primary antibodies -
Description
Rabbit monoclonal [EPR11890] to PSIP1/LEDGF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human PSIP1/LEDGF aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: O75475 -
Positive control
- WB: HEK293T, Jurkat, HeLa and K562 cell lysates. IHC-P: Human brain and hepatocellular carcinoma tissues. ICC/IF: K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11890 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 60 kDa).
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/250.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 60 kDa). |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
Target
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Function
Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration. -
Tissue specificity
Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions. -
Involvement in disease
Note=A chromosomal aberration involving PSIP1 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with NUP98. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4. -
Sequence similarities
Belongs to the HDGF family.
Contains 1 PWWP domain. -
Domain
Residues 340-417 are necessary and sufficient for the interaction with HIV-1 IN (IBD domain). -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Remains chromatin-associated throughout the cell cycle. - Information by UniProt
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Database links
- Entrez Gene: 11168 Human
- Omim: 603620 Human
- SwissProt: O75475 Human
- Unigene: 726445 Human
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Alternative names
- CLL associated antigen KW 7 antibody
- CLL-associated antigen KW-7 antibody
- Dense fine speckles 70 kDa protein antibody
see all
Images
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Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)All lanes : Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : PSIP1 knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 60 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab177159 observed at 75 kDa. Red - loading control ab8245 observed at 36 kDa.
ab177159 Anti-PSIP1/LEDGF antibody [EPR11890] was shown to specifically react with PSIP1/LEDGF in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266464 (knockout cell lysate ab258144) was used. Wild-type and PSIP1/LEDGF knockout samples were subjected to SDS-PAGE. ab177159 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)](https://www.abcam.com/ps/products/177/ab177159/Images/ab177159-205179-anti-psip1-antibody-epr11890-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)Immunofluorescent analysis of K562 cells labeling PSIP1/LEDGF with ab177159 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)](https://www.abcam.com/ps/products/177/ab177159/Images/ab177159-205178-anti-psip1-antibody-epr11890-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling PSIP1/LEDGF with ab177159 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)](https://www.abcam.com/ps/products/177/ab177159/Images/ab177159-205177-anti-psip1-antibody-epr11890-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PSIP1/LEDGF with ab177159 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)](https://www.abcam.com/ps/products/177/ab177159/Images/ab177159-205176-anti-psip1-antibody-epr11890-western-blot.jpg)
Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)All lanes : Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 60 kDa -
![Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)](https://www.abcam.com/ps/products/177/ab177159/Images/ab177159-6-benefits-of-recombinant-antibodies.png)
Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab177159 has been referenced in 1 publication.
- Li L & Wang Y Cross-talk between the H3K36me3 and H4K16ac histone epigenetic marks in DNA double-strand break repair. J Biol Chem 292:11951-11959 (2017). PubMed: 28546430