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  1. Link

    products/primary-antibodies/psip1ledgf-antibody-epr11890-ab177159.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus HIV
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)

  • Datasheet
  • SDS
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Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
  • Immunocytochemistry/ Immunofluorescence - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
  • Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
  • Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11890] to PSIP1/LEDGF
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
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Anti-NSUN5 antibody (ab121633)
Knockout
Product image
Human PSIP1 (LEDGF) knockout HEK-293T cell line (ab266464)
Protein
Recombinant Human PSIP1/LEDGF protein (ab82128)

View more associated products

Overview

  • Product name

    Anti-PSIP1/LEDGF antibody [EPR11890]
    See all PSIP1/LEDGF primary antibodies
  • Description

    Rabbit monoclonal [EPR11890] to PSIP1/LEDGF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human PSIP1/LEDGF aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O75475

  • Positive control

    • WB: HEK293T, Jurkat, HeLa and K562 cell lysates. IHC-P: Human brain and hepatocellular carcinoma tissues. ICC/IF: K562 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11890
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • HIV
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors

Associated products

  • Alternative Versions

    • Anti-PSIP1/LEDGF antibody [EPR11890] - BSA and Azide free (ab249943)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human PSIP1 (LEDGF) knockout HEK-293T cell line (ab266464)
  • KO cell lysates

    • Human PSIP1 (LEDGF) knockout HEK-293T cell lysate (ab258144)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human PSIP1/LEDGF protein (ab82128)
  • Related Products

    • Recombinant Human PSIP1/LEDGF protein (ab82126)
    • Recombinant Human PSIP1/LEDGF protein (ab82128)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab177159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 60 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 60 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.
  • Tissue specificity

    Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions.
  • Involvement in disease

    Note=A chromosomal aberration involving PSIP1 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with NUP98. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
  • Sequence similarities

    Belongs to the HDGF family.
    Contains 1 PWWP domain.
  • Domain

    Residues 340-417 are necessary and sufficient for the interaction with HIV-1 IN (IBD domain).
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Remains chromatin-associated throughout the cell cycle.
  • Target information above from: UniProt accession O75475 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 11168 Human
    • Omim: 603620 Human
    • SwissProt: O75475 Human
    • Unigene: 726445 Human
    • Alternative names

      • CLL associated antigen KW 7 antibody
      • CLL-associated antigen KW-7 antibody
      • Dense fine speckles 70 kDa protein antibody
      • DFS 70 antibody
      • DFS70 antibody
      • LEDGF antibody
      • Lens epithelium derived growth factor antibody
      • Lens epithelium-derived growth factor antibody
      • MGC74712 antibody
      • p52 antibody
      • p75 antibody
      • PAIP antibody
      • PC4 and SFRS1 interacting protein 1 antibody
      • PC4 and SFRS1 interacting protein 2 antibody
      • PC4 and SFRS1 interacting protein antibody
      • PC4 and SFRS1-interacting protein antibody
      • PSIP 1 antibody
      • PSIP 2 antibody
      • Psip1 antibody
      • PSIP1_HUMAN antibody
      • PSIP2 antibody
      • Transcriptional coactivator p75/p52 antibody
      see all

    Images

    • Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
      Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
      All lanes : Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T cell lysate
      Lane 2 : PSIP1 knockout HEK293T cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 60 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?



      Lanes 1-3: Merged signal (red and green). Green - ab177159 observed at 75 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab177159 Anti-PSIP1/LEDGF antibody [EPR11890] was shown to specifically react with PSIP1/LEDGF in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266464 (knockout cell lysate ab258144) was used. Wild-type and PSIP1/LEDGF knockout samples were subjected to SDS-PAGE. ab177159 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
      Immunocytochemistry/ Immunofluorescence - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)

      Immunofluorescent analysis of K562 cells labeling PSIP1/LEDGF with ab177159 at 1/100 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)

      Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling PSIP1/LEDGF with ab177159 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)

      Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PSIP1/LEDGF with ab177159 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
      Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
      All lanes : Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

      Predicted band size: 60 kDa

    • Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
      Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab177159? Please let us know so that we can cite the reference in this datasheet.

    ab177159 has been referenced in 1 publication.

    • Li L & Wang Y Cross-talk between the H3K36me3 and H4K16ac histone epigenetic marks in DNA double-strand break repair. J Biol Chem 292:11951-11959 (2017). PubMed: 28546430

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