Recombinant Anti-PTGES2/Gbf1 antibody [EPR25169-3] (ab300052)
![Western blot - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)](https://www.abcam.com/ps/products/300/ab300052/Images/AB300052-485526-ab300052wb1.jpg "Western blot - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25169-3] to PTGES2/Gbf1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PTGES2/Gbf1 antibody [EPR25169-3]
See all PTGES2/Gbf1 primary antibodies -
Description
Rabbit monoclonal [EPR25169-3] to PTGES2/Gbf1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human heart, Human kidney, Mouse heart, Mouse kidney, Mouse thymus, Rat heart and Rat lung lysates. IHC-P: Human kidney, Human colon carcinoma, Mouse cardiac muscle and Rat cardiac muscle tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25169-3 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/1000. Detects a band of approximately 30, 30, 45 kDa (predicted molecular weight: 42 kDa).
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| Notes |
|---|
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/1000. Detects a band of approximately 30, 30, 45 kDa (predicted molecular weight: 42 kDa). |
Target
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Function
Isomerase that catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form. May also have transactivation activity toward IFN-gamma (IFNG), possibly via an interaction with CEBPB; however, the relevance of transcription activation activity remains unclear. -
Tissue specificity
Widely expressed. Expressed in the heart, including apex, inter-ventricular septum, both atria and ventricles, but not in the aorta. Also expressed in fetal heart. Detected in various regions of the brain: cerebellum; occipital, frontal and parietal lobes. Also expressed in the lymph nodes, skeletal muscle, kidney and trachea, but not in the thymus or lung. Overexpressed in colorectal cancer. -
Pathway
Lipid metabolism; prostaglandin biosynthesis. -
Sequence similarities
Belongs to the GST superfamily.
Contains 1 glutaredoxin domain.
Contains 1 GST C-terminal domain. -
Cellular localization
Golgi apparatus membrane and Cytoplasm > perinuclear region. Synthetized as a Golgi membrane-bound protein, which is further cleaved into the predominant soluble truncated form. The truncated form is cytoplasmic and is enriched in the perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 80142 Human
- Entrez Gene: 96979 Mouse
- Entrez Gene: 311865 Rat
- Omim: 608152 Human
- SwissProt: Q9H7Z7 Human
- SwissProt: Q8BWM0 Mouse
- Unigene: 495219 Human
- Unigene: 28048 Mouse
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Alternative names
- C9orf15 antibody
- FLJ14038 antibody
- Gamma interferon activated transcriptional element binding factor 1 antibody
see all
Images
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Western blot - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)All lanes :
Lane 1 : Human heart tissue lysate
Lane 2 : Human kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 48 secondsBlocking, diluting buffer and concentration was 5% NFDM/TBST.
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![Western blot - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)](https://www.abcam.com/ps/products/300/ab300052/Images/AB300052-485529-ab300052wb2.jpg)
Western blot - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)All lanes : Anti-PTGES2/Gbf1 antibody [EPR25169-3] (ab300052) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse thymus tissue lysate
Lane 4 : Rat heart tissue lysate
Lane 5 : Rat lung tissue lysate
Lysates/proteins at 1/20 dilution per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 30, 45 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsBlocking, diluting buffer and concentration was 5% NFDM/TBST.
The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 12835322).
Low expression: mouse thymus, rat lung (PMID: 15744024, 11866447). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)](https://www.abcam.com/ps/products/300/ab300052/Images/AB300052-2-anti-ptges2gbf1-antibody-epr251693-immunohistochemistry-human-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling PTGES2/Gbf1 with ab300052 at 1/500 (0.944 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human renal tubules. The section was incubated with ab300052 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)](https://www.abcam.com/ps/products/300/ab300052/Images/AB300052-3-anti-ptges2gbf1-antibody-epr251693-immunohistochemistry-human-colon-carcinom.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling PTGES2/Gbf1 with ab300052 at 1/500 (0.944 µ/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human colon carcinoma. The section was incubated with ab300052 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)](https://www.abcam.com/ps/products/300/ab300052/Images/AB300052-4-anti-ptges2gbf1-antibody-epr251693-immunohistochemistry-mouse-cardiac-muscle.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labelling PTGES2/Gbf1 with ab300052 at 1/100 (4.72 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse cardiac muscle. The section was incubated with ab300052 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)](https://www.abcam.com/ps/products/300/ab300052/Images/AB300052-5-anti-ptges2gbf1-antibody-epr251693-immunohistochemistry-rat-cardiac-muscle.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labelling PTGES2/Gbf1 with ab300052 at 1/100 (4.72 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on rat cardiac muscle. The section was incubated with ab300052 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)](https://www.abcam.com/ps/products/300/ab300052/Images/AB300052-485299-Benefits-of-Recombinant-antibodies.jpg)
Anti-PTGES2/Gbf1 antibody [EPR25169-3] (AB300052)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab300052 has not yet been referenced specifically in any publications.