Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-PTGR1 antibody [EPR13451-10] (ab181131)

Submit a review Submit a question References (1)
Western blot - Anti-PTGR1 antibody [EPR13451-10] (ab181131)
  • Western blot - Anti-PTGR1 antibody [EPR13451-10] (ab181131)
  • Western blot - Anti-PTGR1 antibody [EPR13451-10] (ab181131)
  • Anti-PTGR1 antibody [EPR13451-10] (ab181131)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13451-10] to PTGR1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-PTGR1 antibody [EPR13451-10]
    See all PTGR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13451-10] to PTGR1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HT-29 and HeLa cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/2000. Detects a band of approximately 36 kDa (predicted molecular weight: 35 kDa).
Notes
WB
1/1000 - 1/2000. Detects a band of approximately 36 kDa (predicted molecular weight: 35 kDa).

Target

  • Function

    Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-oxo-PGE1, 15-oxo-PGE2 and 15-oxo-PGE2-alpha. Has no activity towards PGE1, PGE2 and PGE2-alpha (By similarity). Catalyzes the conversion of leukotriene B4 into its biologically less active metabolite, 12-oxo-leukotriene B4. This is an initial and key step of metabolic inactivation of leukotriene B4.

  • Tissue specificity

    High expression in the kidney, liver, and intestine but not in leukocytes.

  • Sequence similarities

    Belongs to the NADP-dependent oxidoreductase L4BD family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 15-oxoprostaglandin 13-reductase antibody
    • FLJ99229 antibody
    • leukotriene B4 12-hydroxydehydrogenase antibody
    • LTB4DH antibody
    • MGC34943 antibody
    • NADP-dependent leukotriene B4 12-hydroxydehydrogenase antibody
    • PGR1 antibody
    • PRG-1 antibody
    • Prostaglandin reductase 1 antibody
    • Ptgr1 antibody
    • PTGR1_HUMAN antibody
    • RP11-16L21.1 antibody
    • ZADH3 antibody
    • zinc binding alcohol dehydrogenase domain containing 3 antibody
    see all

Images

  • Western blot - Anti-PTGR1 antibody [EPR13451-10] (ab181131)
    Western blot - Anti-PTGR1 antibody [EPR13451-10] (ab181131)
    All lanes : Anti-PTGR1 antibody [EPR13451-10] (ab181131) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PTGR1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 35 kDa
    Observed band size: 38 kDa why is the actual band size different from the predicted?



    Lanes 1-2: Merged signal (red and green). Green - ab181131 observed at 38 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab181131 Anti-PTGR1 antibody [EPR13451-10] was shown to specifically react with PTGR1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265169 (knockout cell lysate ab258152) was used. Wild-type and PTGR1 knockout samples were subjected to SDS-PAGE. ab181131 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PTGR1 antibody [EPR13451-10] (ab181131)
    Western blot - Anti-PTGR1 antibody [EPR13451-10] (ab181131)
    Anti-PTGR1 antibody [EPR13451-10] (ab181131) at 1/2000 dilution + HT-29 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-PTGR1 antibody [EPR13451-10] (ab181131)
    Western blot - Anti-PTGR1 antibody [EPR13451-10] (ab181131)
    Anti-PTGR1 antibody [EPR13451-10] (ab181131) at 1/1000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?

  • Anti-PTGR1 antibody [EPR13451-10] (ab181131)
    Anti-PTGR1 antibody [EPR13451-10] (ab181131)

References (1)

Publishing research using ab181131? Please let us know so that we can cite the reference in this datasheet.

ab181131 has been referenced in 1 publication.

  • Su Z  et al. Analysis and Identification of Tumorigenic Targets of MicroRNA in Cancer Cells by Photoreactive Chemical Probes. Int J Mol Sci 21:N/A (2020). PubMed: 32102467

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab181131.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com