Anti-PTN antibody [EP3040(2)] (ab133517)
![Western blot - Anti-PTN antibody [EP3040(2)] (ab133517)](https://www.abcam.com/ps/products/133/ab133517/Images/ab133517-147210-anti-ptn-antibody-ep30402-western-blot.jpg "Western blot - Anti-PTN antibody [EP3040(2)] (ab133517)")
Key features and details
- Rabbit monoclonal [EP3040(2)] to PTN
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-PTN antibody [EP3040(2)]
See all PTN primary antibodies -
Description
Rabbit monoclonal [EP3040(2)] to PTN -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human PTN aa 1-100. The exact sequence is proprietary.
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Positive control
- Fetal brain and Fetal cerebellum lysates
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP3040(2) -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 19 kDa).
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 19 kDa). |
Target
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Function
Heparin binding mitogenic protein. Has neurite extension activity. -
Tissue specificity
Osteoblast and brain. -
Sequence similarities
Belongs to the pleiotrophin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5764 Human
- Entrez Gene: 19242 Mouse
- Entrez Gene: 24924 Rat
- Omim: 162095 Human
- SwissProt: P21246 Human
- SwissProt: P63089 Mouse
- SwissProt: P63090 Rat
- Unigene: 371249 Human
see all -
Alternative names
- HARP antibody
- HB-GAM antibody
- HBBM antibody
see all
Images
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Western blot - Anti-PTN antibody [EP3040(2)] (ab133517)All lanes : Anti-PTN antibody [EP3040(2)] (ab133517) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Fetal cerebellum lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 19 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab133517 has been referenced in 1 publication.
- Zhang X et al. LncRNA MACC1-AS1 sponges multiple miRNAs and RNA-binding protein PTBP1. Oncogenesis 8:73 (2019). PubMed: 31822653
