Anti-PTN antibody [EP3040(2)] - BSA and Azide free (ab284804)
Key features and details
- Rabbit monoclonal [EP3040(2)] to PTN - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-PTN antibody [EP3040(2)] - BSA and Azide free
See all PTN primary antibodies -
Description
Rabbit monoclonal [EP3040(2)] to PTN - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fetal brain and Fetal cerebellum lysates
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General notes
ab284804 is the carrier-free version of ab133517
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP3040(2) -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab284804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 19 kDa. |
Target
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Function
Heparin binding mitogenic protein. Has neurite extension activity. -
Tissue specificity
Osteoblast and brain. -
Sequence similarities
Belongs to the pleiotrophin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5764 Human
- Omim: 162095 Human
- SwissProt: P21246 Human
- Unigene: 371249 Human
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Alternative names
- HARP antibody
- HB-GAM antibody
- HBBM antibody
see all
Images
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All lanes : Anti-PTN antibody [EP3040(2)] (ab133517) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Fetal cerebellum lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 19 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?This data was developed using ab133517, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab284804 has not yet been referenced specifically in any publications.