Anti-PXR antibody (ab192579)
Key features and details
- Rabbit polyclonal to PXR
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-PXR antibody
See all PXR primary antibodies -
Description
Rabbit polyclonal to PXR -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Rhesus monkey
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Immunogen
Recombinant full length protein corresponding to Human PXR aa 1 to the C-terminus.
Database link: O75469 -
Positive control
- WB: Rat liver
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab192579 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/500 - 1/20000. Predicted molecular weight: 50 kDa.
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| Notes |
|---|
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WB
1/500 - 1/20000. Predicted molecular weight: 50 kDa. |
Target
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Function
Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes. -
Tissue specificity
Expressed in liver, colon and small intestine. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 18171 Mouse
- Entrez Gene: 84385 Rat
- SwissProt: O54915 Mouse
- SwissProt: Q9R1A7 Rat
- SwissProt: Q8SQ01 Rhesus monkey
- Unigene: 8509 Mouse
- Unigene: 207164 Rat
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Alternative names
- BXR antibody
- Nr1i2 antibody
- NR1I2_HUMAN antibody
see all
Images
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Western blot - Anti-PXR antibody (ab192579)All lanes : Anti-PXR antibody (ab192579) at 1/500 dilution
Lane 1 : Mouse liver
Lane 2 : Rat liver
Predicted band size: 50 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab192579 has been referenced in 8 publications.
- Huang XX et al. Eurotium cristatum Fermented Loose Dark Tea Ameliorates Cigarette Smoke-Induced Lung Injury by MAPK Pathway and Enhances Hepatic Metabolic Detoxification by PXR/AhR Pathway in Mice. Oxid Med Cell Longev 2021:6635080 (2021). PubMed: 33777316
- Bartolini D et al. Garcinoic Acid Is a Natural and Selective Agonist of Pregnane X Receptor. J Med Chem 63:3701-3712 (2020). PubMed: 32160459
- Zeng H et al. CYP3A suppression during diet-induced nonalcoholic fatty liver disease is independent of PXR regulation. Chem Biol Interact 308:185-193 (2019). PubMed: 31132328
- Yang L et al. Panax ginseng Inhibits Metabolism of Diester Alkaloids by Downregulating CYP3A4 Enzyme Activity via the Pregnane X Receptor. Evid Based Complement Alternat Med 2019:3508658 (2019). PubMed: 31057647
- Gao XJ et al. Bacterial Outer Membrane Vesicles from Dextran Sulfate Sodium-Induced Colitis Differentially Regulate Intestinal UDP-Glucuronosyltransferase 1A1 Partially Through Toll-Like Receptor 4/Mitogen-Activated Protein Kinase/Phosphatidylinositol 3-Kinase Pathway. Drug Metab Dispos 46:292-302 (2018). PubMed: 29311138
- Liu M et al. Activating the pregnane X receptor by imperatorin attenuates dextran sulphate sodium-induced colitis in mice. Br J Pharmacol 175:3563-3580 (2018). PubMed: 29945292
- Shubbar MH & Penny JI Effect of amyloid beta on ATP-binding cassette transporter expression and activity in porcine brain microvascular endothelial cells. Biochim Biophys Acta 1862:2314-2322 (2018). WB . PubMed: 30048740
- Guo J et al. Meclizine Prevents Ovariectomy-Induced Bone Loss and Inhibits Osteoclastogenesis Partially by Upregulating PXR. Front Pharmacol 8:693 (2017). PubMed: 29046637