Anti-Quinone oxidoreductase antibody [2A9DD1] (ab110310)
![Immunocytochemistry/ Immunofluorescence - Anti-Quinone oxidoreductase antibody [2A9DD1] (ab110310)](https://www.abcam.com/ps/products/110/ab110310/Images/CRYZ-Primary-antibodies-ab110310-7.gif "Immunocytochemistry/ Immunofluorescence - Anti-Quinone oxidoreductase antibody [2A9DD1] (ab110310)")
Key features and details
- Mouse monoclonal [2A9DD1] to Quinone oxidoreductase
- Suitable for: ICC/IF, IP
- Reacts with: Human
- Isotype: IgG2a
Overview
-
Product name
Anti-Quinone oxidoreductase antibody [2A9DD1]
See all Quinone oxidoreductase primary antibodies -
Description
Mouse monoclonal [2A9DD1] to Quinone oxidoreductase -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
-
Positive control
- HeLa cells; Human liver and HepG2 lysates.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purification notes
ab110310 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
2A9DD1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 2 µg/ml. For 2 hours.
|
|
| IP |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 2 µg/ml. For 2 hours. |
|
IP
Use at an assay dependent concentration. |
Target
-
Function
Does not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding. -
Tissue specificity
Only very low amounts in the lens. -
Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 1429 Human
- Omim: 123691 Human
- SwissProt: Q08257 Human
- Unigene: 83114 Human
-
Alternative names
- Crystallin zeta (quinone reductase) antibody
- Crystallin zeta antibody
- Cryz antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Quinone oxidoreductase antibody [2A9DD1] (ab110310)
Immunocytochemistry analysis using ab110310 at 2µg/ml staining Quinone oxidoreductase in HELa cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green).
-
![Immunoprecipitation - Anti-Quinone oxidoreductase antibody [2A9DD1] (ab110310)](https://www.abcam.com/ps/products/110/ab110310/Images/CRYZ-Primary-antibodies-ab110310-8.gif)
Immunoprecipitation - Anti-Quinone oxidoreductase antibody [2A9DD1] (ab110310)Using MitoSciences' standard immunoprecipitation protocol, ab110310 precipitates the 35kDa Quinone oxidoreductase protein from human liver and HepG2 lysate. Identity of this protein was confirmed by mass spectrometry. This gel was stained with silver nitrate.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab110310 has not yet been referenced specifically in any publications.