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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-RAB10 antibody [EPR13242] - BSA and Azide free (ab224886)

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Western blot - Anti-RAB10 antibody [EPR13242] - BSA and Azide free (ab224886)
  • Flow Cytometry (Intracellular) - Anti-RAB10 antibody [EPR13242] - BSA and Azide free (ab224886)
  • Western blot - Anti-RAB10 antibody [EPR13242] - BSA and Azide free (ab224886)
  • Western blot - Anti-RAB10 antibody [EPR13242] - BSA and Azide free (ab224886)
  • Anti-RAB10 antibody [EPR13242] - BSA and Azide free (ab224886)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13242] to RAB10 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-RAB10 antibody [EPR13242] - BSA and Azide free
    See all RAB10 primary antibodies

  • Description

    Rabbit monoclonal [EPR13242] to RAB10 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human RAB10 aa 50-200. The exact sequence is proprietary.
    Database link: P61026

  • Positive control

    • WB: NIH/3T3, C6, U-87 MG, and HeLa whole cell lysates Flow Cyt (intra): HeLa cells.

  • General notes

    ab224886 is the carrier-free version of ab181367.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab224886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.

Target

  • Function

    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. According to PubMed:23263280, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion.

  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.

  • Cellular localization

    Cytoplasmic vesicle membrane. Golgi apparatus membrane. Golgi apparatus, trans-Golgi network membrane. Endosome membrane. Recycling endosome membrane. Cytoplasmic vesicle, phagosome membrane. Cell projection, cilium. Endoplasmic reticulum membrane. Associates with SLC2A4/GLUT4 storage vesicles (PubMed:22908308). Localizes to the base of the cilium (PubMed:20576682). Transiently associates with phagosomes (By similarity). Localizes to the endoplasmic reticulum at domains of new tubule growth (PubMed:23263280).
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • GTP binding protein RAB10 antibody
    • Rab10 antibody
    • RAB10 member RAS oncogene family antibody
    • RAB10_HUMAN antibody
    • Ras related GTP binding protein antibody
    • Ras related GTP binding protein RAB10 antibody
    • Ras-related protein Rab-10 antibody
    see all

Images

  • Western blot - Anti-RAB10 antibody [EPR13242] - BSA and Azide free (ab224886)
    Western blot - Anti-RAB10 antibody [EPR13242] - BSA and Azide free (ab224886)
    All lanes : Anti-RAB10 antibody [EPR13242] (ab181367) at 1/1000 dilution

    Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 2 : RAB10 knockout A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab181367 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab181367 was shown to specifically react with RAB10 in wild-type A549 cells as signal was lost in RAB10 knockout cells. Wild-type and RAB10 knockout samples were subjected to SDS-PAGE. Ab181367 and ab8245(Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181367).

  • Flow Cytometry (Intracellular) - Anti-RAB10 antibody [EPR13242] - BSA and Azide free (ab224886)
    Flow Cytometry (Intracellular) - Anti-RAB10 antibody [EPR13242] - BSA and Azide free (ab224886)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RAB10 with Purified ab181367 at 1/20 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181367). 

     

     

  • Western blot - Anti-RAB10 antibody [EPR13242] - BSA and Azide free (ab224886)
    Western blot - Anti-RAB10 antibody [EPR13242] - BSA and Azide free (ab224886)
    All lanes : Anti-RAB10 antibody [EPR13242] (ab181367) at 1/1000 dilution

    Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181367).

  • Western blot - Anti-RAB10 antibody [EPR13242] - BSA and Azide free (ab224886)
    Western blot - Anti-RAB10 antibody [EPR13242] - BSA and Azide free (ab224886)
    All lanes : Anti-C21orf33 antibody [EPR13213] (ab181366) at 1/2000 dilution (Purified)

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181367).

  • Anti-RAB10 antibody [EPR13242] - BSA and Azide free (ab224886)
    Anti-RAB10 antibody [EPR13242] - BSA and Azide free (ab224886)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab224886? Please let us know so that we can cite the reference in this datasheet.

ab224886 has not yet been referenced specifically in any publications.

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