Recombinant Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060)
![Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060)](https://www.abcam.com/ps/products/241/ab241060/Images/ab241060-479218-241060.png "Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MJF-R21-22-5] to RAB10 (phospho T73)
- Suitable for: WB, Dot blot, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5]
See all RAB10 primary antibodies -
Description
Rabbit monoclonal [MJF-R21-22-5] to RAB10 (phospho T73) -
Host species
Rabbit -
Tested applications
Suitable for: WB, Dot blot, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293 overexpressing HA-tagged Rab10, with MLi2 treatment whole cell lysate, 293T transfected with RAB10 expression vector containing a myc-His-tag®, whole cell lysate. ICC/IF: MLi-2 treated MEF cells. Dot Blot: Rab10 (phospho T73) peptide.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This antibody was developed with support from The Michael J. Fox Foundation.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MJF-R21-22-5 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab241060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
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| Dot blot |
1/1000.
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| ICC/IF |
1/100.
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| Notes |
|---|
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WB
1/500. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa). |
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Dot blot
1/1000. |
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ICC/IF
1/100. |
Target
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Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. According to PubMed:23263280, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Cytoplasmic vesicle membrane. Golgi apparatus membrane. Golgi apparatus, trans-Golgi network membrane. Endosome membrane. Recycling endosome membrane. Cytoplasmic vesicle, phagosome membrane. Cell projection, cilium. Endoplasmic reticulum membrane. Associates with SLC2A4/GLUT4 storage vesicles (PubMed:22908308). Localizes to the base of the cilium (PubMed:20576682). Transiently associates with phagosomes (By similarity). Localizes to the endoplasmic reticulum at domains of new tubule growth (PubMed:23263280). - Information by UniProt
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Database links
- Entrez Gene: 10890 Human
- Entrez Gene: 19325 Mouse
- Omim: 612672 Human
- SwissProt: P61026 Human
- SwissProt: P61027 Mouse
- Unigene: 467960 Human
- Unigene: 378993 Mouse
- Unigene: 486858 Mouse
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Alternative names
- GTP binding protein RAB10 antibody
- Rab10 antibody
- RAB10 member RAS oncogene family antibody
see all
Images
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Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060)All lanes : Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060) at 1/200 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with an empty vector, containing a myc-His-tag®, whole cell lysate
Lane 2 : 293T transfected with RAB10 expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Predicted band size: 22 kDa
Exposure time: 60 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Observed MW 28kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060)](https://www.abcam.com/ps/products/241/ab241060/Images/ab241060-332557-anti-rab10-phospho-t73-antibody-mjf-r21-22-5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Saponin permeabilized MEF (mouse embryonic fibroblast) generated from wild-type or mutant LRRK2[R1441C] embryos without and with (+) MLi-2 treatment (100nM for 60 min) cells labeling RAB10 (phospho T73) with ab241060 at 0.5 μg/mL, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) secondary antibody at 1/1000 dilution (red). Nuclear counterstain is DAPI.
The image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee.
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![Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060)](https://www.abcam.com/ps/products/241/ab241060/Images/ab241060-332558-anti-rab10-phospho-t73-antibody-mjf-r21-22-5-western-blot.jpg)
Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060)All lanes : Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060) at 1/500 dilution
Lane 1 : HEK293 (human embryonic kidney epithelial cells) overexpressing HA-tagged Rab3, whole cell lysate
Lane 2 : HEK293 overexpressing HA-tagged Rab3, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate
Lane 3 : HEK293 overexpressing HA-tagged Rab5, whole cell lysate
Lane 4 : HEK293 overexpressing HA-tagged Rab5, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate
Lane 5 : HEK293 overexpressing HA-tagged Rab8, whole cell lysate
Lane 6 : HEK293 overexpressing HA-tagged Rab8, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate
Lane 7 : HEK293 overexpressing HA-tagged Rab10, whole cell lysate
Lane 8 : HEK293 overexpressing HA-tagged Rab10, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate
Lane 9 : HEK293 overexpressing HA-tagged Rab12, whole cell lysate
Lane 10 : HEK293 overexpressing HA-tagged Rab12, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate
Lane 11 : HEK293 overexpressing HA-tagged Rab29, whole cell lysate
Lane 12 : HEK293 overexpressing HA-tagged Rab29, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate
Lane 13 : HEK293 overexpressing HA-tagged Rab35, whole cell lysate
Lane 14 : HEK293 overexpressing HA-tagged Rab35, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate
Lane 15 : HEK293 overexpressing HA-tagged Rab43, whole cell lysate
Lane 16 : HEK293 overexpressing HA-tagged Rab43, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate
Lysates/proteins at 3 µg per lane.
Secondary
All lanes : IRDye® 800CW Goat-anti-Rabbit at 1/25000 dilution
Predicted band size: 22 kDaBlocking buffer: 5% NFDM/TBST.
Dilution buffer: 5% BSA/TBST.
MLi-2 is a potent, selective LARRK2 kinase inhibitor (PMID: 26407721).
The blot was scanned with LI-COR Odyssey CLx.
This image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee.
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![Dot Blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060)](https://www.abcam.com/ps/products/241/ab241060/Images/ab241060-332559-anti-rab10-phospho-t73-antibody-mjf-r21-22-5-dot-blot.jpg)
Dot Blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060)Dot Blot - Anti-RAB10 antibody [MJF-R21-22-5] (ab241060) at a 1/1000 dilution.
Lane 1: Rab10 (phospho T73) peptide
Lane 2: Rab10 non-phospho peptide
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1/100000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 180 seconds.
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![Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060)](https://www.abcam.com/ps/products/241/ab241060/Images/ab241060-4-benefits-of-recombinant-antibodies.png)
Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab241060 has been referenced in 2 publications.
- De Miranda BR et al. The industrial solvent trichloroethylene induces LRRK2 kinase activity and dopaminergic neurodegeneration in a rat model of Parkinson's disease. Neurobiol Dis 153:105312 (2021). PubMed: 33636387
- Bonet-Ponce L et al. LRRK2 mediates tubulation and vesicle sorting from lysosomes. Sci Adv 6:N/A (2020). PubMed: 33177079