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    products/primary-antibodies/rab11a-antibody-epr7587b-ab128913.pdf

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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family
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RecombinantRabMAb

Recombinant Anti-Rab11A antibody [EPR7587(B)] (ab128913)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (10)

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Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
  • Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
  • Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
  • Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
  • Immunoprecipitation - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
  • Anti-Rab11A antibody [EPR7587(B)] (ab128913)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7587(B)] to Rab11A
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

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Carrier Free

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Overview

  • Product name

    Anti-Rab11A antibody [EPR7587(B)]
    See all Rab11A primary antibodies
  • Description

    Rabbit monoclonal [EPR7587(B)] to Rab11A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: PC12, HeLa and A549 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7587(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Cancer
    • Signal transduction
    • G protein signaling
    • Small G proteins
    • Ras family
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Rab11A antibody [EPR7587(B)] - BSA and Azide free (ab240036)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa membrane extract lysate (ab29547)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/10000 - 1/50000. Predicted molecular weight: 24 kDa.
IP
1/10 - 1/100.
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 24 kDa.
IP
1/10 - 1/100.
Application notes
Is unsuitable for ICC/IF or IHC-P.

Target

  • Function

    Regulates endocytic recycling. May exert its functions by interacting with multiple effector proteins in different complexes. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (By similarity). Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization

    Cell membrane. Recycling endosome membrane. Cleavage furrow. Translocates with RAB11FIP2 from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane. Localizes to the cleavage furrow. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis.
  • Target information above from: UniProt accession P62491 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8766 Human
    • Entrez Gene: 53869 Mouse
    • Entrez Gene: 81830 Rat
    • Omim: 605570 Human
    • SwissProt: P62491 Human
    • SwissProt: P62492 Mouse
    • SwissProt: P62494 Rat
    • Unigene: 321541 Human
    • Unigene: 1387 Mouse
    • Unigene: 1016 Rat
    see all
  • Alternative names

    • MGC1490 antibody
    • Rab 11 antibody
    • Rab 11A antibody
    • RAB 11A, member oncogene family antibody
    • Rab-11 antibody
    • RAB11 A antibody
    • RAB11 antibody
    • Rab11a antibody
    • RAB11A member RAS oncogene family antibody
    • Ras related protein Rab 11A antibody
    • Ras related protein Rab11A antibody
    • Ras-related protein Rab-11A antibody
    • RB11A_HUMAN antibody
    • YL 8 antibody
    • YL8 antibody
    see all

Images

  • Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    All lanes : Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution (unpurified)

    Lane 1 : PC12 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 24 kDa

  • Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    All lanes : Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution (purified)

    Lane 1 : Rat brain lysate
    Lane 2 : PC-12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    All lanes : Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution (purified)

    Lane 1 : Mouse brain cell lysate
    Lane 2 : Mouse spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa

  • Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution (purified) + A549 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunoprecipitation - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    Immunoprecipitation - Anti-Rab11A antibody [EPR7587(B)] (ab128913)

    ab128913 (purified) at 1/20 immunoprecipitating Rab11A in A549 whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/10,000) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    Anti-Rab11A antibody [EPR7587(B)] (ab128913)

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (10)

Publishing research using ab128913? Please let us know so that we can cite the reference in this datasheet.

ab128913 has been referenced in 10 publications.

  • Dembla E & Becherer U Biogenesis of large dense core vesicles in mouse chromaffin cells. Traffic 22:78-93 (2021). PubMed: 33369005
  • Mao D  et al. LncRNA SNHG6 Induces Epithelial-Mesenchymal Transition of Pituitary Adenoma Via Suppressing MiR-944. Cancer Biother Radiopharm N/A:N/A (2020). PubMed: 32935999
  • Kumar GA  et al. Exploring Endocytosis and Intracellular Trafficking of the Human Serotonin1A Receptor. Biochemistry 58:2628-2641 (2019). PubMed: 30896156
  • Zhu S  et al. Rab11a-Rab8a cascade regulates the formation of tunneling nanotubes through vesicle recycling. J Cell Sci 131:N/A (2018). PubMed: 30209134
  • Puri C  et al. The RAB11A-Positive Compartment Is a Primary Platform for Autophagosome Assembly Mediated by WIPI2 Recognition of PI3P-RAB11A. Dev Cell 45:114-131.e8 (2018). WB, ICC/IF . PubMed: 29634932
  • Li H  et al. Coronary Serum Exosomes Derived from Patients with Myocardial Ischemia Regulate Angiogenesis through the miR-939-mediated Nitric Oxide Signaling Pathway. Theranostics 8:2079-2093 (2018). PubMed: 29721064
  • Jackson TC  et al. Whole-transcriptome microarray analysis reveals regulation of Rab4 by RBM5 in neurons. Neuroscience 361:93-107 (2017). PubMed: 28818525
  • Vogel GF  et al. Disrupted apical exocytosis of cargo vesicles causes enteropathy in FHL5 patients with Munc18-2 mutations. JCI Insight 2:N/A (2017). PubMed: 28724787
  • Lee H  et al. Lung Epithelial Cell-Derived Microvesicles Regulate Macrophage Migration via MicroRNA-17/221-Induced Integrin ß1 Recycling. J Immunol 199:1453-1464 (2017). PubMed: 28674181
  • Vogel GF  et al. Cargo-selective apical exocytosis in epithelial cells is conducted by Myo5B, Slp4a, Vamp7, and Syntaxin 3. J Cell Biol 211:587-604 (2015). PubMed: 26553929

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Rab11A antibody [EPR7587(B)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (cortex)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
cortex
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 10 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab11A antibody [EPR7587(B)]

Good
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (9 days in vitro primary cortical neurons from E14.)
Permeabilization
Yes - 0.1% Triton
Specification
9 days in vitro primary cortical neurons from E14.
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 24 2021

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