Recombinant Anti-Rab11A antibody [EPR7587(B)] (ab128913)
![Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)](https://www.abcam.com/ps/products/128/ab128913/Images/ab128913-142740-anti-rab11a-antibody-epr7587b-western-blot.jpg "Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7587(B)] to Rab11A
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Rab11A antibody [EPR7587(B)]
See all Rab11A primary antibodies -
Description
Rabbit monoclonal [EPR7587(B)] to Rab11A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: PC12, HeLa and A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7587(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 24 kDa.
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| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 24 kDa. |
|
IP
1/10 - 1/100. |
Application notes
Is unsuitable for ICC/IF or IHC-P.
Target
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Function
Regulates endocytic recycling. May exert its functions by interacting with multiple effector proteins in different complexes. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (By similarity). Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Cell membrane. Recycling endosome membrane. Cleavage furrow. Translocates with RAB11FIP2 from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane. Localizes to the cleavage furrow. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis. - Information by UniProt
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Database links
- Entrez Gene: 8766 Human
- Entrez Gene: 53869 Mouse
- Entrez Gene: 81830 Rat
- Omim: 605570 Human
- SwissProt: P62491 Human
- SwissProt: P62492 Mouse
- SwissProt: P62494 Rat
- Unigene: 321541 Human
see all -
Alternative names
- MGC1490 antibody
- Rab 11 antibody
- Rab 11A antibody
see all
Images
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Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)All lanes : Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution (unpurified)
Lane 1 : PC12 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 24 kDa -
![Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)](https://www.abcam.com/ps/products/128/ab128913/Images/ab128913-251259-anti-rab11a-antibody-epr7587b-western-blot.jpg)
Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)All lanes : Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution (purified)
Lane 1 : Rat brain lysate
Lane 2 : PC-12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 24 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)](https://www.abcam.com/ps/products/128/ab128913/Images/ab128913-251258-anti-rab11a-antibody-epr7587b-western-blot.jpg)
Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)All lanes : Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution (purified)
Lane 1 : Mouse brain cell lysate
Lane 2 : Mouse spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 24 kDa -
![Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)](https://www.abcam.com/ps/products/128/ab128913/Images/ab128913-251257-anti-rab11a-antibody-epr7587b-western-blot.jpg)
Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution (purified) + A549 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 24 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Immunoprecipitation - Anti-Rab11A antibody [EPR7587(B)] (ab128913)](https://www.abcam.com/ps/products/128/ab128913/Images/ab128913-251229-anti-rab11a-antibody-epr7587b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Rab11A antibody [EPR7587(B)] (ab128913)ab128913 (purified) at 1/20 immunoprecipitating Rab11A in A549 whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/10,000) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Anti-Rab11A antibody [EPR7587(B)] (ab128913)](https://www.abcam.com/ps/products/128/ab128913/Images/ab128913-6-benefits-of-recombinant-antibodies.png)
Anti-Rab11A antibody [EPR7587(B)] (ab128913)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab128913 has been referenced in 10 publications.
- Dembla E & Becherer U Biogenesis of large dense core vesicles in mouse chromaffin cells. Traffic 22:78-93 (2021). PubMed: 33369005
- Mao D et al. LncRNA SNHG6 Induces Epithelial-Mesenchymal Transition of Pituitary Adenoma Via Suppressing MiR-944. Cancer Biother Radiopharm N/A:N/A (2020). PubMed: 32935999
- Kumar GA et al. Exploring Endocytosis and Intracellular Trafficking of the Human Serotonin1A Receptor. Biochemistry 58:2628-2641 (2019). PubMed: 30896156
- Zhu S et al. Rab11a-Rab8a cascade regulates the formation of tunneling nanotubes through vesicle recycling. J Cell Sci 131:N/A (2018). PubMed: 30209134
- Puri C et al. The RAB11A-Positive Compartment Is a Primary Platform for Autophagosome Assembly Mediated by WIPI2 Recognition of PI3P-RAB11A. Dev Cell 45:114-131.e8 (2018). WB, ICC/IF . PubMed: 29634932
- Li H et al. Coronary Serum Exosomes Derived from Patients with Myocardial Ischemia Regulate Angiogenesis through the miR-939-mediated Nitric Oxide Signaling Pathway. Theranostics 8:2079-2093 (2018). PubMed: 29721064
- Jackson TC et al. Whole-transcriptome microarray analysis reveals regulation of Rab4 by RBM5 in neurons. Neuroscience 361:93-107 (2017). PubMed: 28818525
- Vogel GF et al. Disrupted apical exocytosis of cargo vesicles causes enteropathy in FHL5 patients with Munc18-2 mutations. JCI Insight 2:N/A (2017). PubMed: 28724787
- Lee H et al. Lung Epithelial Cell-Derived Microvesicles Regulate Macrophage Migration via MicroRNA-17/221-Induced Integrin ß1 Recycling. J Immunol 199:1453-1464 (2017). PubMed: 28674181
- Vogel GF et al. Cargo-selective apical exocytosis in epithelial cells is conducted by Myo5B, Slp4a, Vamp7, and Syntaxin 3. J Cell Biol 211:587-604 (2015). PubMed: 26553929