Recombinant Anti-RAB22A antibody [EPR9486] (ab137093)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9486] to RAB22A
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-RAB22A antibody [EPR9486]
See all RAB22A primary antibodies -
Description
Rabbit monoclonal [EPR9486] to RAB22A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human RAB22A aa 1-100. The exact sequence is proprietary.
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Positive control
- WB: MCF-7 and BxPC-3 cell lysate; fetal kidney and fetal heart tissue lysates ICC/IF: MCF-7 cells. IP: MCF-7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9486 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
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ICC/IF |
1/100.
For unpurified use at 1/250 - 1/500. |
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 22 kDa. |
ICC/IF
1/100. For unpurified use at 1/250 - 1/500. |
IP
1/10 - 1/100. |
Target
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Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Endosome membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 57403 Human
- Entrez Gene: 19334 Mouse
- Entrez Gene: 366265 Rat
- Omim: 612966 Human
- SwissProt: Q9UL26 Human
- SwissProt: P35285 Mouse
- Unigene: 529044 Human
- Unigene: 275304 Mouse
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Alternative names
- 3732413A17Rik antibody
- AI662177 antibody
- AW319644 antibody
see all
Images
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All lanes : Anti-RAB22A antibody [EPR9486] (ab137093) at 1/1000 dilution (Purified)
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa -
Anti-RAB22A antibody [EPR9486] (ab137093) at 1/1000 dilution (Purified) + Human brain lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa -
All lanes : Anti-RAB22A antibody [EPR9486] (ab137093) at 1/1000 dilution (unpurified)
Lane 1 : MCF-7 lysate
Lane 2 : Fetal kidney lysate
Lane 3 : BxPC-3 lysate
Lane 4 : Fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 22 kDa -
ab137093 (purified) at 1/40 dilution (2ug) immunoprecipitating RAB22A in MCF7 whole cell lysates.
Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates 10ug
Lane 2 (+): ab137093 & MCF7 whole cell lysates
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137093 in MCF7 whole cell lysates
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling RAB22A with purified ab137093 at 1/100 dilution (7.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab137093 has been referenced in 12 publications.
- Gao Y et al. Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity. Cell Res 32:1086-1104 (2022). PubMed: 36280710
- Blum IR et al. Rab22a regulates the establishment of epithelial polarity. Small GTPases 12:282-293 (2021). PubMed: 32281471
- Tu J et al. Circular RNA Circ0021205 Promotes Cholangiocarcinoma Progression Through MiR-204-5p/RAB22A Axis. Front Cell Dev Biol 9:653207 (2021). PubMed: 34012964
- Ivanova D et al. CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles. Cell Rep 30:2444-2459.e7 (2020). PubMed: 32075774
- Tang X et al. LncRNA ZFAS1 confers inflammatory responses and reduces cholesterol efflux in atherosclerosis through regulating miR-654-3p-ADAM10/RAB22A axis. Int J Cardiol N/A:N/A (2020). PubMed: 32349937