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RecombinantRabMAb

Recombinant Anti-Rab5 antibody [EPR17321] (ab199530)

Submit a review Submit a question References (2)
Western blot - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
  • Western blot - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
  • Immunocytochemistry/ Immunofluorescence - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
  • Western blot - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
  • Western blot - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
  • Anti-RAB5C/RABL antibody [EPR17321] (ab199530)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17321] to Rab5
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free HRP

Overview

  • Product name

    Anti-Rab5 antibody [EPR17321]
    See all Rab5 primary antibodies

  • Description

    Rabbit monoclonal [EPR17321] to Rab5

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293, HepG2 and U937 cell lysates; Human fetal heart lysate, Recombinant his-tagged RAB5 protein IHC-P: Human kidney tissue. ICC/IF: HepG2 and MCF7 cells,

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab199530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.
Notes
WB
1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.

Target

  • Function

    Required for the fusion of plasma membranes and early endosomes.

  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.

  • Cellular localization

    Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes.
  • Information by UniProt

  • Database links

    • Alternative names

      • RAB 5 antibody
      • RAB 5A antibody
      • RAB5A antibody
      • RAB5A member RAS oncogene family antibody
      • RAB5A_HUMAN antibody
      • RAS associated protein RAB5A antibody
      • Ras related protein Rab 5A antibody
      • Ras-related protein Rab-5A antibody
      see all

    Images

    • Western blot - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
      Western blot - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
      All lanes : Anti-Rab5 antibody [EPR17321] (ab199530) at 1/1000 dilution

      Lane 1 : Recombinant Human Rab5A protein (ab62956) with NFDM/ TBST
      Lane 2 : Recombinant Human Rab5b protein (ab104545) with NFDM/ TBST
      Lane 3 : Recombinant Human RAB5C/RABL protein (ab156358) with NFDM/ TBST

      Blocking peptides at 5 % per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 23 kDa
      Observed band size: 26 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
      Western blot - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
      All lanes : Anti-Rab5 antibody [EPR17321] (ab199530) at 1/1000 dilution

      Lane 1 : HEK293 (Human embryonic kidney) cell lysate
      Lane 2 : HepG2 (Human liver hepatocellular carcinoma) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 23 kDa
      Observed band size: 23 kDa


      Exposure time: 10 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
      Immunocytochemistry/ Immunofluorescence - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)

      Ab199530 staining RAB5C/RABL in HepG2 cells (Human hepatocellular carcinoma epithelial cell) by Immunocytochemistry/Immunofluorescence (ICC/IF). Cells were fixed with 100% Methanol. Samples were incubated with primary antibody at 1:500 dilution (2.2µg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution (2µg/ml). An Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594), ab195889 was used as a counterstain antibody at 1:200 dilution (2.5µg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic staining in HepG2 cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling RAB5C/RABL with ab199530 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining of Human kidney tissue is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody and secondary antibody Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
      Western blot - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
      Anti-Rab5 antibody [EPR17321] (ab199530) at 1/10000 dilution + U937 (Human histiocytic lymphoma cells) cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 23 kDa
      Observed band size: 23 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
      Western blot - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
      Anti-Rab5 antibody [EPR17321] (ab199530) at 1/1000 dilution + Human fetal heart tissue lysate at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 23 kDa
      Observed band size: 23 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
      Anti-RAB5C/RABL antibody [EPR17321] (ab199530)

    References (2)

    Publishing research using ab199530? Please let us know so that we can cite the reference in this datasheet.

    ab199530 has been referenced in 2 publications.

    • Li J  et al. Long Noncoding RNA NRAV Promotes Respiratory Syncytial Virus Replication by Targeting the MicroRNA miR-509-3p/Rab5c Axis To Regulate Vesicle Transportation. J Virol 94:N/A (2020). PubMed: 32102886
    • Manna PT  et al. Fast and cloning-free CRISPR/Cas9-mediated genomic editing in mammalian cells. Traffic 20:974-982 (2019). PubMed: 31503392

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