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RecombinantRabMAb

Recombinant Anti-Rab5 antibody [EPR17321] - BSA and Azide free (ab251279)

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Western blot - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)
  • Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody [EPR17321] - BSA and Azide free (ab251279)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)
  • Western blot - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)
  • Western blot - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)
  • Western blot - Anti-Rab5 antibody [EPR17321] - BSA and Azide free (ab251279)
  • Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17321] to Rab5 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 HRP Unconjugated

Overview

  • Product name

    Anti-Rab5 antibody [EPR17321] - BSA and Azide free
    See all Rab5 primary antibodies

  • Description

    Rabbit monoclonal [EPR17321] to Rab5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251279 is the carrier-free version of ab199530.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab251279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Required for the fusion of plasma membranes and early endosomes.

  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.

  • Cellular localization

    Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes.
  • Information by UniProt

  • Database links

    • Alternative names

      • RAB 5 antibody
      • RAB 5A antibody
      • RAB5A antibody
      • RAB5A member RAS oncogene family antibody
      • RAB5A_HUMAN antibody
      • RAS associated protein RAB5A antibody
      • Ras related protein Rab 5A antibody
      • Ras-related protein Rab-5A antibody
      see all

    Images

    • Western blot - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)
      Western blot - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)
      All lanes : Anti-Rab5 antibody [EPR17321] (ab199530) at 1/1000 dilution

      Lane 1 : HEK293 (Human embryonic kidney) cell lysate
      Lane 2 : HepG2 (Human liver hepatocellular carcinoma) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 23 kDa
      Observed band size: 23 kDa


      Exposure time: 10 seconds


      This data was developed using ab199530, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody [EPR17321] - BSA and Azide free (ab251279)
      Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody [EPR17321] - BSA and Azide free (ab251279)

      This data was developed using ab199530, the same antibody clone in a different buffer formulation.

      Ab199530 staining RAB5C/RABL in HepG2 cells (Human hepatocellular carcinoma epithelial cell) by Immunocytochemistry/Immunofluorescence (ICC/IF). Cells were fixed with 100% Methanol. Samples were incubated with primary antibody at 1:500 dilution (2.2µg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution (2µg/ml). An Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594), ab195889 was used as a counterstain antibody at 1:200 dilution (2.5µg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic staining in HepG2 cells.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)
      This data was developed using ab199530, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling RAB5C/RABL with ab199530 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining of Human kidney tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody and secondary antibody Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Western blot - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)
      Western blot - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)
      Anti-Rab5 antibody [EPR17321] (ab199530) at 1/10000 dilution + U937 (Human histiocytic lymphoma cells) cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 23 kDa
      Observed band size: 23 kDa


      Exposure time: 3 minutes


      This data was developed using ab199530, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)
      Western blot - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)
      Anti-Rab5 antibody [EPR17321] (ab199530) at 1/1000 dilution + Human fetal heart tissue lysate at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 23 kDa
      Observed band size: 23 kDa


      Exposure time: 3 minutes


      This data was developed using ab199530, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Rab5 antibody [EPR17321] - BSA and Azide free (ab251279)
      Western blot - Anti-Rab5 antibody [EPR17321] - BSA and Azide free (ab251279)
      All lanes : Anti-Rab5 antibody [EPR17321] (ab199530) at 1/1000 dilution

      Lane 1 : Recombinant Human Rab5A protein (ab62956) with NFDM/ TBST
      Lane 2 : Recombinant Human Rab5b protein (ab104545) with NFDM/ TBST
      Lane 3 : Recombinant Human RAB5C/RABL protein (ab156358) with NFDM/ TBST

      Blocking peptides at 5 % per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 23 kDa
      Observed band size: 26 kDa why is the actual band size different from the predicted?



      This data was developed using ab199530, the same antibody clone in a different buffer formulation.

    • Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)
      Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab251279? Please let us know so that we can cite the reference in this datasheet.

    ab251279 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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