Anti-Rab5A antibody - C-terminal (ab228770)
Key features and details
- Rabbit polyclonal to Rab5A - C-terminal
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Rab5A antibody - C-terminal
See all Rab5A primary antibodies -
Description
Rabbit polyclonal to Rab5A - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Dog, Rhesus monkey
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Immunogen
Synthetic peptide within Human Rab5A (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (Carrier-protein conjugated).
Database link: P20339 -
Positive control
- WB: A431 and Raji whole cell lysate; Mouse brain lysate. IP: HeLa whole cell lysate. IHC-P: U-87 MG xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 24 kDa.
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| IP |
1/100 - 1/500.
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| IHC-P |
1/100 - 1/1000.
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| Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 24 kDa. |
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IP
1/100 - 1/500. |
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IHC-P
1/100 - 1/1000. |
Target
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Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Cell membrane. Early endosome membrane. Melanosome. Cytoplasmic vesicle. Cell projection, ruffle. Membrane. Cytoplasm, cytosol. Cytoplasmic vesicle, phagosome membrane. Enriched in stage I melanosomes (PubMed:17081065). Alternates between membrane-bound and cytosolic forms (Probable). - Information by UniProt
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Database links
- Entrez Gene: 5868 Human
- SwissProt: P20339 Human
- Unigene: 475663 Human
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Alternative names
- RAB5A antibody
- RAB5A_HUMAN antibody
- Ras-related protein Rab-5A antibody
Images
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Western blot - Anti-Rab5A antibody - C-terminal (ab228770)All lanes : Anti-Rab5A antibody - C-terminal (ab228770) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 24 kDa12% SDS-PAGE gel.
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Immunoprecipitation - Anti-Rab5A antibody - C-terminal (ab228770)Rab5 was immunoprecipitated from 1 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab228770. Western blot was performed from the immunoprecipitate using ab228770 at 1/1000 dilution. Anti-rabbit IgG was used as secondary antibody.
Lane 1: HeLa whole cell lysate 30 µg (Input).
Lane 2: Control IP with 2 μg of preimmune rabbit IgG in HeLa whole cell lysate.
Lane 3: ab228770 IP in HeLa whole cell lysate.
15% SDS-PAGE gel.
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Western blot - Anti-Rab5A antibody - C-terminal (ab228770)Anti-Rab5A antibody - C-terminal (ab228770) at 1/1000 dilution + Mouse brain lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 24 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5A antibody - C-terminal (ab228770)Paraffin-embedded U-87 MG (human glioblastoma-astrocytoma epithelial cell line) xenograft stained for Rab5 using ab228770 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228770 has not yet been referenced specifically in any publications.