Anti-Rab5b antibody (ab72907)
Key features and details
- Rabbit polyclonal to Rab5b
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Rab5b antibody
See all Rab5b primary antibodies -
Description
Rabbit polyclonal to Rab5b -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Orangutan
-
Immunogen
Synthetic peptide corresponding to Human Rab5b aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab102726) -
Positive control
- This antibody gave a positive signal in the following lysates: Human Placenta Tissue; HeLa Whole Cell; Jurkat Whole Cell; HEK293 Whole Cell; HepG2 Whole Cell; Human Brain Tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
|
| WB |
Use a concentration of 1 mg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
WB
Use a concentration of 1 mg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa). |
Target
-
Relevance
Rab5b is a member of the Rab family of small (monomeric) G proteins. Like other small G proteins, Rab5b switches between an inactive, GDP-form and an active, GTP-bound form. GDP/GTP exchange factors (GEFs) catalyse the conversion from the GDP-bound form to the GTP-bound form, while GTPase-activating proteins (GAPs) catalyse GTP hydrolysis to GDP. Rab5b is involved in endocytosis and recycling of cell surface molecules. It interacts with RIN2 and RIN3, which regulate its function, possibly by acting as GEFs. Knockdown of Rab5b abolished group I metabotropic glutamate receptor (mGluR)-mediated neuroprotection. Furthermore, Rab5b interacts with LRRK2, the defective gene at the PARK8 locus that results in Parkinson's disease. Roles for Rab5b in neurodegenerative disease, neuroprotection, and synaptic plasticity have been suggested. -
Cellular localization
Cell Membrane, Endosome -
Database links
- Entrez Gene: 5869 Human
- Entrez Gene: 19344 Mouse
- Entrez Gene: 100172422 Orangutan
- Entrez Gene: 288779 Rat
- Omim: 179514 Human
- SwissProt: P61020 Human
- SwissProt: P61021 Mouse
- SwissProt: Q5RBG1 Orangutan
-
Alternative names
- Rab 5b antibody
- Rab5 b antibody
- RAB5B, member RAS oncogene family antibody
see all
Images
-
Western blot - Anti-Rab5b antibody (ab72907)All lanes : Anti-Rab5b antibody (ab72907) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 70 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Rab5b contains a number of potential lipidation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunocytochemistry/ Immunofluorescence - Anti-Rab5b antibody (ab72907)ICC/IF image of ab72907 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72907, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HepG2 cells at 5µg/ml.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab72907 has been referenced in 2 publications.
- Li XM et al. Long non-coding RNA MIAT promotes gastric cancer proliferation and metastasis via modulating the miR-331-3p/RAB5B pathway. Oncol Lett 20:355 (2020). PubMed: 33154765
- Connor-Robson N et al. An integrated transcriptomics and proteomics analysis reveals functional endocytic dysregulation caused by mutations in LRRK2. Neurobiol Dis 127:512-526 (2019). PubMed: 30954703