Recombinant Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16709] to RABL3 - C-terminal
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-RABL3 antibody [EPR16709] - C-terminal
See all RABL3 primary antibodies -
Description
Rabbit monoclonal [EPR16709] to RABL3 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- MCF7, K562 and 293 cell lysates. Human renal adenocarcinoma and rat liver tissues. A549 and MCF7 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16709 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF |
1/100.
|
|
WB |
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
|
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
Notes |
---|
Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/100. |
WB
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. - Information by UniProt
-
Database links
- Entrez Gene: 285282 Human
- Entrez Gene: 67657 Mouse
- Entrez Gene: 360720 Rat
- SwissProt: Q5HYI8 Human
- SwissProt: Q9D4V7 Mouse
- Unigene: 444360 Human
-
Alternative names
- RAB member of RAS oncogene family like 3 antibody
- Rab-like protein 3 antibody
- RABL3 antibody
- RABL3_HUMAN antibody
Images
-
Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024) at 1/10000 dilution + MCF7 cell lysate at 20 µg
Secondary
Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 26 kDa -
All lanes : Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 26 kDa -
Immunohistochemical analysis of paraffin-embedded, human renal adenocarcinoma tissue labeling RABL3 with ab196024 at a 1/100 dilution. Counter stained with hematoxylin. As secondary antibody goat anti-rabbit IgG H&L (HRP) ab97051 was used at a 1/500. In blue DAPI staining.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded, rat liver tissue labeling RABL3 with ab196024 at a 1/100 dilution. Counter stained with hematoxylin. As secondary antibody goat anti-rabbit IgG H&L (HRP) ab97051 was used at a 1/500. In blue DAPI staining.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunofluorescence analysis of paraformaldehyde-fixed A549 cells labeling RABL3 with ab196024 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/500. In blue DAPI staining.
-
Intracellular Flow Cytometry analysis of paraformaldehyde-fixed MCF7 cells labeling RABL3 with ab196024 at a 1/100 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, isotype control rabbit IgG (black) and unlabeled control cell without incubation with primary antibody and secondary antibody (Blue).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab196024 has not yet been referenced specifically in any publications.