Anti-Rabphilin 3A antibody (ab3338)
Key features and details
- Rabbit polyclonal to Rabphilin 3A
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Rabphilin 3A antibody
See all Rabphilin 3A primary antibodies -
Description
Rabbit polyclonal to Rabphilin 3A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Rat Rabphilin 3A aa 1-18.
Sequence:MTDTVVNRWMYPGDGPLQ
(Peptide available asab4954) -
Positive control
- WB: Rat Brain ; Mouse Brain ICC/IF: RSC96
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml.
By Western blot, this antibody detects an ~75 kDa protein representing Rabphilin 3A from rat brain whole protein extract. |
| ICC/IF |
Use a concentration of 2 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. By Western blot, this antibody detects an ~75 kDa protein representing Rabphilin 3A from rat brain whole protein extract. |
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ICC/IF
Use a concentration of 2 µg/ml. |
Target
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Function
Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal. -
Sequence similarities
Contains 2 C2 domains.
Contains 1 FYVE-type zinc finger.
Contains 1 RabBD (Rab-binding) domain. -
Cellular localization
Cell junction > synapse. - Information by UniProt
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Database links
- Entrez Gene: 19894 Mouse
- Entrez Gene: 171039 Rat
- SwissProt: P47708 Mouse
- SwissProt: P47709 Rat
- Unigene: 181166 Mouse
- Unigene: 10976 Rat
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Alternative names
- Exophilin 1 antibody
- Exophilin-1 antibody
- Exophilin1 antibody
see all
Images
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Western blot - Anti-Rabphilin 3A antibody (ab3338)All lanes : Anti-Rabphilin 3A antibody (ab3338) at 3 µg/ml
Lane 1 : Mouse Brain
Lane 2 : Rat Brain
Lane 3 : Rat Lung
Lane 4 : Rat Liver
Lane 5 : Rat Kidney
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-Rabbit IgG at 1/4000 dilution
Additional bands at: ~76 kDa. We are unsure as to the identity of these extra bands.Detection: chemiluminescence
Rabphilin 3A observed in Mouse and Rat Brain, and not observed in other tissues which are documented to be Rabphilin 3A negative.
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Western blot - Anti-Rabphilin 3A antibody (ab3338)Anti-Rabphilin 3A antibody (ab3338) at 2 µg/ml + Rat Brain
Secondary
HRP conjugated Goat anti-Rabbit IgG at 1/2500 dilution
Additional bands at: ~76 kDa. We are unsure as to the identity of these extra bands.Detection: chemiluminescence
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Western blot - Anti-Rabphilin 3A antibody (ab3338)Western blot detection of Rabphilin 3A in rat brain extract using ab3338.
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Immunocytochemistry/ Immunofluorescence - Anti-Rabphilin 3A antibody (ab3338)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton™ X-100 permeabilized, 70% confluent log phase RSC96 (rat neuronal Schwann cell) cells, blocked with 3% BSA-PBS for 1 hour at room temperature. Labelling Rabphilin 3A with ab3338 at 2 µg/mL and incubated for 3 hours at room temperature then labelled with secondary antibody goat anti-rabbit igG, Alexa Fluor® 488 conjugate at a 1/2000 dilution for 45 minutes at room temperature (Green). Nuclei stained with DAPI (Blue). F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin at a dilution of 1/300 (Red). Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Datasheets and documents
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Datasheet download
References (3)
ab3338 has been referenced in 3 publications.
- Selma-Soriano E et al. Rabphilin silencing causes dilated cardiomyopathy in a Drosophila model of nephrocyte damage. Sci Rep 11:15287 (2021). PubMed: 34315987
- Selma-Soriano E et al. Rabphilin involvement in filtration and molecular uptake in Drosophila nephrocytes suggests a similar role in human podocytes. Dis Model Mech 13:N/A (2020). PubMed: 32680845
- Xu XQ et al. S1PR1 mediates anti-apoptotic/pro-proliferative processes in human acute myeloid leukemia cells. Mol Med Rep 14:3369-75 (2016). PubMed: 27572094
