Anti-Rad21 antibody (ab992)
Key features and details
- Rabbit polyclonal to Rad21
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
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Overview
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Product name
Anti-Rad21 antibody
See all Rad21 primary antibodies -
Description
Rabbit polyclonal to Rad21 -
Host species
Rabbit -
Specificity
The epitope recognized by Anti-Rad21 antibody (ab992) maps to a region between residue 575 and the C-terminus (residue 631) human Rad21 homolog using the numbering given in entry NP_006256.1 (GeneID 5885).
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Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Gorilla, Chinese hamster, Orangutan, Elephant
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Immunogen
Synthetic peptide corresponding to Human Rad21 (C terminal) conjugated to keyhole limpet haemocyanin. Represented a portion of human Rad21 encoded within exon 14 (LocusLink ID 5885).
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Positive control
- WB: HeLa, Jurkat, HEK293T, RKO, GaMG, MCF-7, A-549, U2OS, NIH/3T3, and TCMK-1 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | (1) |
Use at an assay dependent concentration.
ab992 used for IP at 6 µg per reaction. |
| WB | (7) |
1/500 - 1/5000. Predicted molecular weight: 72 kDa.
Band observed at ~130 kDa. |
| Notes |
|---|
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IP
Use at an assay dependent concentration. ab992 used for IP at 6 µg per reaction. |
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WB
1/500 - 1/5000. Predicted molecular weight: 72 kDa. Band observed at ~130 kDa. |
Target
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Function
Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. -
Sequence similarities
Belongs to the rad21 family. -
Domain
The C-terminal part associates with the head of SMC1A, while the N-terminal part binds to the head of SMC3. -
Post-translational
modificationsCleaved by separase/ESPL1 at the onset of anaphase. Cleaved by caspase-3 and caspase-7 at the beginning of apoptosis. The cleavage by ESPL1 and caspase-3 take place at different sites.
Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK. -
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis. - Information by UniProt
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Database links
- Entrez Gene: 540966 Cow
- Entrez Gene: 5885 Human
- Entrez Gene: 19357 Mouse
- Entrez Gene: 314949 Rat
- Omim: 606462 Human
- SwissProt: Q3SWX9 Cow
- SwissProt: O60216 Human
- SwissProt: Q61550 Mouse
see all -
Alternative names
- CDLS4 antibody
- Double-strand-break repair protein rad21 homolog antibody
- hHR21 antibody
see all
Images
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Western blot - Anti-Rad21 antibody (ab992)All lanes : Anti-Rad21 antibody (ab992) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : RKO (human cell line from colon carcinoma) whole cell lysate
Lane 5 : GaMG (human cell line from glioblastoma) whole cell lysate
Lane 6 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 7 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 8 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 9 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 10 : TCMK-1 (mouse epithelial kidney cell line transformed with SV40) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 72 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-Rad21 antibody (ab992)Immunoprecipitation analysis of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa (human epithelial cell line from cervix adenocarcinoma) cells prepared using NETN lysis buffer.
Lane 1: ab992 used for IP at 6 µg per reaction
Lane 2: Control IgG
For blotting immunoprecipitated Rad21, a rabbit anti-Rad21 recombinant monoclonal antibody was used at 1/1000. Detected using chemiluminescence with an exposure time of 3 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (163)
ab992 has been referenced in 163 publications.
- Murillo-Pineda M et al. Induction of spontaneous human neocentromere formation and long-term maturation. J Cell Biol 220:N/A (2021). PubMed: 33443568
- Vladimirova O et al. Phase separation and DAXX redistribution contribute to LANA nuclear body and KSHV genome dynamics during latency and reactivation. PLoS Pathog 17:e1009231 (2021). PubMed: 33471863
- Li X et al. Cohesin promotes HSV-1 lytic transcription by facilitating the binding of RNA Pol II on viral genes. Virol J 18:26 (2021). PubMed: 33485391
- Laffleur B et al. Noncoding RNA processing by DIS3 regulates chromosomal architecture and somatic hypermutation in B cells. Nat Genet 53:230-242 (2021). PubMed: 33526923
- Luan J et al. Distinct properties and functions of CTCF revealed by a rapidly inducible degron system. Cell Rep 34:108783 (2021). PubMed: 33626344