Recombinant Anti-RAMP1 antibody [EPR10867] (ab156575)
![Western blot - Anti-RAMP1 antibody [EPR10867] (ab156575)](https://www.abcam.com/ps/products/156/ab156575/Images/ab156575-298947-anti-ramp1-antibody-epr10867-western-blot.jpg "Western blot - Anti-RAMP1 antibody [EPR10867] (ab156575)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10867] to RAMP1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-RAMP1 antibody [EPR10867]
See all RAMP1 primary antibodies -
Description
Rabbit monoclonal [EPR10867] to RAMP1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human RAMP1 aa 100-200. The exact sequence is proprietary.
Database link: O60894 -
Positive control
- WB: Human uterus, fetal brain, mouse brain, rat brain, human uterus and Caco-2 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10867 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Target
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Function
Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for calcitonin-gene-related peptide (CGRP) together with CALCRL. -
Tissue specificity
Expressed in many tissues including the uterus, bladder, brain, pancreas and gastro-intestinal tract. -
Sequence similarities
Belongs to the RAMP family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10267 Human
- Entrez Gene: 51801 Mouse
- Entrez Gene: 58965 Rat
- Omim: 605153 Human
- SwissProt: O60894 Human
- SwissProt: Q9WTJ5 Mouse
- SwissProt: Q9JJ74 Rat
- Unigene: 471783 Human
see all -
Alternative names
- Calcitonin receptor like receptor activity modifying protein 1 antibody
- Calcitonin-receptor-like receptor activity-modifying protein 1 antibody
- CRLR activity modifying protein 1 antibody
see all
Images
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Western blot - Anti-RAMP1 antibody [EPR10867] (ab156575)Anti-RAMP1 antibody [EPR10867] (ab156575) at 1/1000 dilution (purified) + Human uterus lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 17 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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![Western blot - Anti-RAMP1 antibody [EPR10867] (ab156575)](https://www.abcam.com/ps/products/156/ab156575/Images/ab156575-298943-anti-ramp1-antibody-epr10867-western-blot.jpg)
Western blot - Anti-RAMP1 antibody [EPR10867] (ab156575)All lanes : Anti-RAMP1 antibody [EPR10867] (ab156575) at 1/5000 dilution (purified)
Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 17 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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![Western blot - Anti-RAMP1 antibody [EPR10867] (ab156575)](https://www.abcam.com/ps/products/156/ab156575/Images/ab156575-163714-anti-ramp1-antibody-epr10867-western-blot.jpg)
Western blot - Anti-RAMP1 antibody [EPR10867] (ab156575)All lanes : Anti-RAMP1 antibody [EPR10867] (ab156575) at 1/1000 dilution (unpurified)
Lane 1 : Human uterus lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 17 kDa -
![Anti-RAMP1 antibody [EPR10867] (ab156575)](https://www.abcam.com/ps/products/156/ab156575/Images/ab156575-5-benefits-of-recombinant-antibodies.png)
Anti-RAMP1 antibody [EPR10867] (ab156575)
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab156575 has been referenced in 12 publications.
- Yin S et al. Receptor activity-modifying protein 1 regulates mouse skin fibroblast proliferation via the Gαi3-PKA-CREB-YAP axis. Cell Commun Signal 20:52 (2022). PubMed: 35413847
- Hu B et al. Sensory Nerve Maintains Intervertebral Disc Extracellular Matrix Homeostasis Via CGRP/CHSY1 Axis. Adv Sci (Weinh) 9:e2202620 (2022). PubMed: 36047655
- Ochoa-Callejero L et al. Circulating Levels of Calcitonin Gene-Related Peptide Are Lower in COVID-19 Patients. J Endocr Soc 5:bvaa199 (2021). PubMed: 33506161
- Aranäs C et al. Salmon Calcitonin Attenuates Some Behavioural Responses to Nicotine in Male Mice. Front Pharmacol 12:685631 (2021). PubMed: 34234676
- Stöckl S et al. Substance P and Alpha-Calcitonin Gene-Related Peptide Differentially Affect Human Osteoarthritic and Healthy Chondrocytes. Front Immunol 12:722884 (2021). PubMed: 34512650