Recombinant Anti-Ran antibody [EPR10791(B)] - BSA and Azide free (ab249192)
![Western blot - Anti-Ran antibody [EPR10791(B)] - BSA and Azide free (ab249192)](https://www.abcam.com/ps/products/249/ab249192/Images/ab249192-396963-anti-ran-antibody-epr10791-b-westernblot-human-mouse-rat.jpg "Western blot - Anti-Ran antibody [EPR10791(B)] - BSA and Azide free (ab249192)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10791(B)] to Ran - BSA and Azide free
- Suitable for: IP, Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Ran antibody [EPR10791(B)] - BSA and Azide free
See all Ran primary antibodies -
Description
Rabbit monoclonal [EPR10791(B)] to Ran - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, Flow Cyt (Intra), WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, LoVo, Jurkat, HeLa, C6, Raw 264.7, PC-12, and NIH/3T3 whole cell lysates; IP: HeLa whole cell lysate; Flow Cyt (intra): HeLa cells; ICC/IF: HeLa cells.
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General notes
ab249192 is the carrier-free version of ab155103.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10791(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
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| ICC/IF |
Use at an assay dependent concentration.
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| Notes |
|---|
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IP
Use at an assay dependent concentration. |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Function
GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases. -
Tissue specificity
Expressed in a variety of tissues. -
Sequence similarities
Belongs to the small GTPase superfamily. Ran family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Nucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 5901 Human
- Entrez Gene: 100045999 Mouse
- Entrez Gene: 19384 Mouse
- Entrez Gene: 84509 Rat
- Omim: 601179 Human
- SwissProt: P62826 Human
- SwissProt: P62827 Mouse
- SwissProt: P62828 Rat
see all -
Alternative names
- Androgen receptor associated protein 24 antibody
- Androgen receptor-associated protein 24 antibody
- ARA 24 antibody
see all
Images
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Western blot - Anti-Ran antibody [EPR10791(B)] - BSA and Azide free (ab249192)All lanes : Anti-Ran antibody [EPR10791(B)] (ab155103) at 1/1000 dilution (purified)
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : LoVo (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 6 : Raw 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 8 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDaWe are unsure how to define the extra bands. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155103).
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![Immunocytochemistry/ Immunofluorescence - Anti-Ran antibody [EPR10791(B)] - BSA and Azide free (ab249192)](https://www.abcam.com/ps/products/249/ab249192/Images/ab249192-3-anti-ran-antibody-epr10791-b-immunocytochemistry-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Ran antibody [EPR10791(B)] - BSA and Azide free (ab249192)Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Ran with purified ab155103 at 1/100 dilution (10 µg/mL). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155103). -
![Flow Cytometry (Intracellular) - Anti-Ran antibody [EPR10791(B)] - BSA and Azide free (ab249192)](https://www.abcam.com/ps/products/249/ab249192/Images/ab249192-2-anti-ran-antibody-epr10791-b-flowcytometry-hela-human.jpg)
Flow Cytometry (Intracellular) - Anti-Ran antibody [EPR10791(B)] - BSA and Azide free (ab249192)Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Ran with purified ab155103 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155103).
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![Immunoprecipitation - Anti-Ran antibody [EPR10791(B)] - BSA and Azide free (ab249192)](https://www.abcam.com/ps/products/249/ab249192/Images/ab249192-1-anti-ran-antibody-epr10791-b-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-Ran antibody [EPR10791(B)] - BSA and Azide free (ab249192)Purified ab155103 at 1/50 dilution (2 µg) immunoprecipitating Ran in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2 (+): ab155103 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab155103 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 24 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155103). -
![Anti-Ran antibody [EPR10791(B)] - BSA and Azide free (ab249192)](https://www.abcam.com/ps/products/249/ab249192/Images/ab249192-5-benefits-of-recombinant-antibodies.png)
Anti-Ran antibody [EPR10791(B)] - BSA and Azide free (ab249192)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249192 has not yet been referenced specifically in any publications.
