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    products/primary-antibodies/rank-antibody-epr26196-15-bsa-and-azide-free-ab305234.pdf

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Immunology Innate Immunity Cytokines TNF Superfamily
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)
  • Western blot - Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)
  • Western blot - Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)
  • Western blot - Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)
  • Western blot - Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)
  • Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26196-15] to RANK - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

HRP

Overview

  • Product name

    Anti-RANK antibody [EPR26196-15] - BSA and Azide free
    See all RANK primary antibodies
  • Description

    Rabbit monoclonal [EPR26196-15] to RANK - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    KO validation is still required when KO cell line or lysates become available.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt (Intra),ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HT-29, SW480, HCT 116, C2C12, Mouse brain, Mouse thymus, K-562, RAW 264.7, Rat thymus, Rat spleen, K-562 non-membrane fraction, K-562 membrane fraction, PC-3 (fresh), MCF7 (fresh), PC-3 (frozen) and MCF7 (frozen) lysates
  • General notes

    ab305234 is the carrier-free version of ab305233 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Constituent: 100% PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR26196-15
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Signal Transduction
    • Adapters
    • Transmembrane
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-RANK antibody [EPR26196-15] (ab305233)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab305234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 80 kDa.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 80 kDa.
Application notes
Is unsuitable for Flow Cyt (Intra),ICC/IF,IHC-P or IP.

Target

  • Function

    Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells.
  • Tissue specificity

    Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.
  • Involvement in disease

    Defects in TNFRSF11A are the cause of familial expansile osteolysis (FEO) [MIM:174810]. FEO is a rare autosomal dominant bone disorder characterized by focal areas of increased bone remodeling. The osteolytic lesions develop usually in the long bones during early adulthood. FEO is often associated with early onset deafness and loss of dentition.
    Defects in TNFRSF11A are a cause of Paget disease of bone type 2 (PDB2) [MIM:602080]; also known as familial Paget disease of bone. PDB2 is a bone-remodeling disorder with clinical similarities to FEO. Unlike FEO, however, affected individuals have involvement of the axial skeleton with lesions in the spine, pelvis and skull.
    Defects in TNFRSF11A are the cause of osteopetrosis autosomal recessive type 7 (OPTB7) [MIM:612301]; also called osteoclast-poor osteopetrosis with hypogammaglobulinemia. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTB7 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. OPTB7 is associated with hypogammaglobulinemia.
  • Sequence similarities

    Contains 4 TNFR-Cys repeats.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q9Y6Q6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8792 Human
    • Entrez Gene: 21934 Mouse
    • Entrez Gene: 498206 Rat
    • Omim: 603499 Human
    • SwissProt: Q9Y6Q6 Human
    • SwissProt: O35305 Mouse
    • Unigene: 204044 Human
    • Unigene: 6251 Mouse
    • Alternative names

      • CD 265 antibody
      • CD265 antibody
      • FEO antibody
      • LOH18CR1 antibody
      • Loss of heterozygosity 18 chromosomal region 1 antibody
      • mRANK antibody
      • ODFR antibody
      • OFE antibody
      • OPTB7 antibody
      • Osteoclast differentiation factor receptor antibody
      • OSTS antibody
      • Paget disease of bone 2 antibody
      • PDB 2 antibody
      • PDB2 antibody
      • RANK antibody
      • Receptor activator of NF KB antibody
      • Receptor activator of NF-KB antibody
      • receptor activator of nuclear factor kappa B antibody
      • TNF receptor superfamily member 11a antibody
      • TNFRSF11A antibody
      • TNR11_HUMAN antibody
      • TRANCER antibody
      • Tumor necrosis factor receptor superfamily member 11a NFKB activator antibody
      • Tumor necrosis factor receptor superfamily member 11a activator of NFKB antibody
      • Tumor necrosis factor receptor superfamily member 11A antibody
      see all

    Images

    • Western blot - Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)
      Western blot - Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)
      All lanes : Anti-RANK antibody [EPR26196-15] (ab305233) at 1/1000 dilution

      Lane 1 : HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate 20 µg
      Lane 2 : SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate 20 µg
      Lane 3 : HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

      Predicted band size: 80 kDa
      Observed band size: 80 kDa



      This data was developed using ab305233, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Low expression: SW480, HCT 116 (PMID:33795653).
      This blot was developed using a high sensitivity ECL substrate.

      Exposure time: 3 minutes

    • Western blot - Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)
      Western blot - Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)
      All lanes : Anti-RANK antibody [EPR26196-15] (ab305233) at 1/1000 dilution

      Lane 1 : C2C12 (mouse myoblast) whole cell lysate 20 µg
      Lane 2 : Mouse brain tissue lysate 20 µg
      Lane 3 : Mouse thymus tissue lysate 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

      Predicted band size: 80 kDa



      This data was developed using ab305233, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      This blot was developed using a high sensitivity ECL substrate.

      Exposure time: 3 minutes

    • Western blot - Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)
      Western blot - Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)
      All lanes : Anti-RANK antibody [EPR26196-15] (ab305233) at 1/1000 dilution

      Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 20 µg
      Lane 2 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 20 µg
      Lane 3 : Rat thymus tissue lysate 20 µg
      Lane 4 : Rat spleen tissue lysate 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

      Predicted band size: 80 kDa
      Observed band size: 80 kDa



      This data was developed using ab305233, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      This blot was developed using a high sensitivity ECL substrate.

      Exposure time: 3 minutes

    • Western blot - Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)
      Western blot - Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)
      All lanes : Anti-RANK antibody [EPR26196-15] (ab305233) at 1/1000 dilution

      Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) non-membrane fraction 20 µg
      Lane 2 : K-562 membrane fraction 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

      Predicted band size: 80 kDa
      Observed band size: 80 kDa


      Exposure time: 3 minutes


      This data was developed using ab305233, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      This blot was developed using a high sensitivity ECL substrate.

      Exposure time: 3 minutes

    • Western blot - Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)
      Western blot - Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)
      All lanes : Anti-RANK antibody [EPR26196-15] (ab305233) at 1/1000 dilution

      Lanes 1 & 3 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate 20 µg
      Lanes 2 & 4 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

      Predicted band size: 80 kDa
      Observed band size: 80 kDa



      This data was developed using ab305233, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Low expression: MCF7 (PMID: 23696795).

      Exposure time:

      Lanes 1-2: 92 seconds;
      Lanes 3-4: 180 seconds.

    • Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)
      Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab305234? Please let us know so that we can cite the reference in this datasheet.

    ab305234 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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