Recombinant Anti-RANK antibody [EPR26196-15] - BSA and Azide free (ab305234)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26196-15] to RANK - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-RANK antibody [EPR26196-15] - BSA and Azide free
See all RANK primary antibodies -
Description
Rabbit monoclonal [EPR26196-15] to RANK - BSA and Azide free -
Host species
Rabbit -
Specificity
KO validation is still required when KO cell line or lysates become available.
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Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HT-29, SW480, HCT 116, C2C12, Mouse brain, Mouse thymus, K-562, RAW 264.7, Rat thymus, Rat spleen, K-562 non-membrane fraction, K-562 membrane fraction, PC-3 (fresh), MCF7 (fresh), PC-3 (frozen) and MCF7 (frozen) lysates
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General notes
ab305234 is the carrier-free version of ab305233
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26196-15 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab305234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 80 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 80 kDa. |
Target
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Function
Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. -
Tissue specificity
Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. -
Involvement in disease
Defects in TNFRSF11A are the cause of familial expansile osteolysis (FEO) [MIM:174810]. FEO is a rare autosomal dominant bone disorder characterized by focal areas of increased bone remodeling. The osteolytic lesions develop usually in the long bones during early adulthood. FEO is often associated with early onset deafness and loss of dentition.
Defects in TNFRSF11A are a cause of Paget disease of bone type 2 (PDB2) [MIM:602080]; also known as familial Paget disease of bone. PDB2 is a bone-remodeling disorder with clinical similarities to FEO. Unlike FEO, however, affected individuals have involvement of the axial skeleton with lesions in the spine, pelvis and skull.
Defects in TNFRSF11A are the cause of osteopetrosis autosomal recessive type 7 (OPTB7) [MIM:612301]; also called osteoclast-poor osteopetrosis with hypogammaglobulinemia. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTB7 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. OPTB7 is associated with hypogammaglobulinemia. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8792 Human
- Entrez Gene: 21934 Mouse
- Entrez Gene: 498206 Rat
- Omim: 603499 Human
- SwissProt: Q9Y6Q6 Human
- SwissProt: O35305 Mouse
- Unigene: 204044 Human
- Unigene: 6251 Mouse
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Alternative names
- CD 265 antibody
- CD265 antibody
- FEO antibody
see all
Images
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All lanes : Anti-RANK antibody [EPR26196-15] (ab305233) at 1/1000 dilution
Lane 1 : HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate 20 µg
Lane 2 : SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate 20 µg
Lane 3 : HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDaThis data was developed using ab305233, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: SW480, HCT 116 (PMID:33795653).
This blot was developed using a high sensitivity ECL substrate.Exposure time: 3 minutes
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All lanes : Anti-RANK antibody [EPR26196-15] (ab305233) at 1/1000 dilution
Lane 1 : C2C12 (mouse myoblast) whole cell lysate 20 µg
Lane 2 : Mouse brain tissue lysate 20 µg
Lane 3 : Mouse thymus tissue lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 80 kDaThis data was developed using ab305233, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate.
Exposure time: 3 minutes
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All lanes : Anti-RANK antibody [EPR26196-15] (ab305233) at 1/1000 dilution
Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 20 µg
Lane 2 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 20 µg
Lane 3 : Rat thymus tissue lysate 20 µg
Lane 4 : Rat spleen tissue lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDaThis data was developed using ab305233, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate.
Exposure time: 3 minutes
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All lanes : Anti-RANK antibody [EPR26196-15] (ab305233) at 1/1000 dilution
Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) non-membrane fraction 20 µg
Lane 2 : K-562 membrane fraction 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDa
Exposure time: 3 minutesThis data was developed using ab305233, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate.
Exposure time: 3 minutes
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All lanes : Anti-RANK antibody [EPR26196-15] (ab305233) at 1/1000 dilution
Lanes 1 & 3 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate 20 µg
Lanes 2 & 4 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDaThis data was developed using ab305233, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: MCF7 (PMID: 23696795).
Exposure time:
Lanes 1-2: 92 seconds;
Lanes 3-4: 180 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab305234 has not yet been referenced specifically in any publications.