Recombinant Anti-RANKL antibody [101] - BSA and Azide free (ab276057)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [101] to RANKL - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-RANKL antibody [101] - BSA and Azide free
See all RANKL primary antibodies -
Description
Rabbit monoclonal [101] to RANKL - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human RANKL aa 63-244.
Database link: O14788-2 -
Positive control
- WB: HEK-293T membrane lysate. IP: Raji whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
101 -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab276057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 35 kDa.
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IP |
Use at an assay dependent concentration.
0.2-1 μL/mg of lysate |
Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 35 kDa. |
IP
Use at an assay dependent concentration. 0.2-1 μL/mg of lysate |
Target
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Function
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. -
Tissue specificity
Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. -
Involvement in disease
Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17. -
Cellular localization
Cytoplasm; Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8600 Human
- Omim: 602642 Human
- SwissProt: O14788 Human
- Unigene: 333791 Human
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Alternative names
- CD254 antibody
- hRANKL2 antibody
- ODF antibody
see all
Images
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RANKL was immunoprecipitated from 0.5 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with 0.5 µL of ab276057 and 15 µl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using ab276057 at 1/500 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution.
Developed using the odssey technique.
Performed under reducing conditions. -
Anti-RANKL antibody [101] - BSA and Azide free (ab276057) at 1/500 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) membrane lysate at 30 µg
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Predicted band size: 35 kDaDeveloped using the Odyssey technique.
Performed under reducing conditions.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab276057 has not yet been referenced specifically in any publications.