Recombinant Anti-RAP80 antibody [EPR5315] (ab124763)
![Western blot - Anti-RAP80 antibody [EPR5315] (ab124763)](https://www.abcam.com/ps/products/124/ab124763/Images/ab124763-138610-anti-rap80-antibody-epr5315-western-blot.jpg "Western blot - Anti-RAP80 antibody [EPR5315] (ab124763)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5315] to RAP80
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RAP80 antibody [EPR5315]
See all RAP80 primary antibodies -
Description
Rabbit monoclonal [EPR5315] to RAP80 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human RAP80 aa 1-100. The exact sequence is proprietary.
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Positive control
- HeLa, Human ovary cancer, 293T and HT-1080 cell lysates; Human testis tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5315 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
|
| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
| Notes |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa). |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
Application notes
Is unsuitable for Flow Cyt or IP.
Target
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Function
Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1. -
Tissue specificity
Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis. -
Sequence similarities
Belongs to the RAP80 family.
Contains 2 UIM (ubiquitin-interacting motif) repeats. -
Domain
The UIM-linker region between the 2 UIM repeats determines the selectivity for 'Lys-63'-linked ubiquitin. The length of the linker is important. The linker reduces the flexibility between the UIM repeats and promotes high-affinity and linkage-selective interactions.
The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas. -
Post-translational
modificationsSumoylated.
Phosphorylated upon DNA damage by ATM or ATR. -
Cellular localization
Nucleus. Localizes at sites of DNA damage at double-strand breaks. - Information by UniProt
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Database links
- Entrez Gene: 51720 Human
- SwissProt: Q96RL1 Human
- Unigene: 232721 Human
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Alternative names
- BRCA1-A complex subunit RAP80 antibody
- Nuclear zinc finger protein RAP80 antibody
- OTTHUMP00000161441 antibody
see all
Images
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Western blot - Anti-RAP80 antibody [EPR5315] (ab124763)All lanes : Anti-RAP80 antibody [EPR5315] (ab124763) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human ovary cancer lysate
Lane 3 : 293T cell lysate
Lane 4 : HT-1080 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 80 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP80 antibody [EPR5315] (ab124763)](https://www.abcam.com/ps/products/124/ab124763/Images/ab124763-138609-anti-rap80-antibody-epr5315-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP80 antibody [EPR5315] (ab124763)ab124763, at 1/250, staining RAP80 in paraffin-embedded Human testis tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-RAP80 antibody [EPR5315] (ab124763)](https://www.abcam.com/ps/products/124/ab124763/Images/ab124763-3-benefits-of-recombinant-antibodies.png)
Anti-RAP80 antibody [EPR5315] (ab124763)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab124763 has been referenced in 6 publications.
- Liu P et al. K63-linked polyubiquitin chains bind to DNA to facilitate DNA damage repair. Sci Signal 11:N/A (2018). PubMed: 29871913
- Renaud E et al. Impaired TIP60-mediated H4K16 acetylation accounts for the aberrant chromatin accumulation of 53BP1 and RAP80 in Fanconi anemia pathway-deficient cells. Nucleic Acids Res 44:648-56 (2016). WB ; Human . PubMed: 26446986
- Zhao MJ et al. Adenovirus-mediated downregulation of the ubiquitin ligase RNF8 sensitizes bladder cancer to radiotherapy. Oncotarget 7:8956-67 (2016). IF . PubMed: 26788910
- Yang Q et al. NRAGE is involved in homologous recombination repair to resist the DNA-damaging chemotherapy and composes a ternary complex with RNF8-BARD1 to promote cell survival in squamous esophageal tumorigenesis. Cell Death Differ 23:1406-16 (2016). PubMed: 27035619
- Hu B et al. HSCARG, a novel regulator of H2A ubiquitination by downregulating PRC1 ubiquitin E3 ligase activity, is essential for cell proliferation. Nucleic Acids Res 42:5582-93 (2014). Human . PubMed: 24711370
- Kakarougkas A et al. Co-operation of BRCA1 and POH1 relieves the barriers posed by 53BP1 and RAP80 to resection. Nucleic Acids Res N/A:N/A (2013). ICC/IF . PubMed: 24013561