Recombinant Anti-Ras antibody [EP1125Y] (ab52939)
![Western blot - Anti-Ras antibody [EP1125Y] (ab52939)](https://www.abcam.com/ps/products/52/ab52939/Images/ab52939-298880-anti-ras-antibody-ep1125y-western-blot.jpg "Western blot - Anti-Ras antibody [EP1125Y] (ab52939)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1125Y] to Ras
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Ras antibody [EP1125Y]
See all Ras primary antibodies -
Description
Rabbit monoclonal [EP1125Y] to Ras -
Host species
Rabbit -
Specificity
ab52939 is predicted to react with H-Ras, N-Ras and K-Ras. -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): PC-12 cells. WB: Jurkat, 293T, RAW 246.7, Neuro-2a, PC-12 and C6 lysates; Human Ras full length protein. ICC/IF: MCF7 cells. IP: Jurkat whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1125Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/30.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/100. |
|
| ICC/IF |
1/500.
|
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| WB | (9) |
1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
For unpurified use at 1/10000- 1/50000. |
| IP | (2) |
1/20.
For unpurified use at 1/30. |
| Notes |
|---|
|
Flow Cyt (Intra)
1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/100. |
|
ICC/IF
1/500. |
|
WB
1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). For unpurified use at 1/10000- 1/50000. |
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IP
1/20. For unpurified use at 1/30. |
Target
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Function
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. -
Involvement in disease
Defects in HRAS are the cause of faciocutaneoskeletal syndrome (FCSS) [MIM:218040]. A rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities.
Defects in HRAS are the cause of congenital myopathy with excess of muscle spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome.
Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
Note=Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors.
Defects in HRAS are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.
Note=Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC). -
Sequence similarities
Belongs to the small GTPase superfamily. Ras family. -
Post-translational
modificationsPalmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation. -
Cellular localization
Cell membrane. Golgi apparatus membrane. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 3265 Human
- Entrez Gene: 3845 Human
- Entrez Gene: 4893 Human
- Entrez Gene: 15461 Mouse
- Entrez Gene: 16653 Mouse
- Entrez Gene: 18176 Mouse
- Entrez Gene: 24525 Rat
- Entrez Gene: 24605 Rat
see all -
Alternative names
- C BAS/HAS antibody
- C HA RAS1 antibody
- C-BAS/HAS antibody
see all
Images
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Western blot - Anti-Ras antibody [EP1125Y] (ab52939)All lanes : Anti-Ras antibody [EP1125Y] (ab52939) at 1/5000 dilution (purified)
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysates
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysates
Lane 3 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysates
Lane 4 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EP1125Y] (ab52939)](https://www.abcam.com/ps/products/52/ab52939/Images/ab52939-298493-anti-ras-antibody-ep1125y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EP1125Y] (ab52939)Immunocytochemistry analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling Ras with Purified ab52939 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Immunoprecipitation - Anti-Ras antibody [EP1125Y] (ab52939)](https://www.abcam.com/ps/products/52/ab52939/Images/ab52939-298862-anti-ras-antibody-ep1125y-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Ras antibody [EP1125Y] (ab52939)ab52939 (purified) at 1:20 dilution (2μg) immunoprecipitating Ras in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate 10μg
Lane 2 (+): ab52939 & Jurkat whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52939 in Jurkat whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Flow Cytometry (Intracellular) - Anti-Ras antibody [EP1125Y] (ab52939)](https://www.abcam.com/ps/products/52/ab52939/Images/ab52939-298508-anti-ras-antibody-ep1125y-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Ras antibody [EP1125Y] (ab52939)Intracellular Flow Cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma cell line) cells labeling Ras with purified ab52939 at 1/30 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Western blot - Anti-Ras antibody [EP1125Y] (ab52939)](https://www.abcam.com/ps/products/52/ab52939/Images/ab52939-298882-anti-ras-antibody-ep1125y-western-blot.jpg)
Western blot - Anti-Ras antibody [EP1125Y] (ab52939)Anti-Ras antibody [EP1125Y] (ab52939) at 1/5000 dilution (purified) + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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![Western blot - Anti-Ras antibody [EP1125Y] (ab52939)](https://www.abcam.com/ps/products/52/ab52939/Images/ab52939-65457-anti-ras-antibody-ep1125y-western-blot.gif)
Western blot - Anti-Ras antibody [EP1125Y] (ab52939)Anti-Ras antibody [EP1125Y] (ab52939) at 1/500000 dilution (unpurified) + C6 (rat glial tumor cell line) cell lysate at 10 µg/ml
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-Ras antibody [EP1125Y] (ab52939)](https://www.abcam.com/ps/products/52/ab52939/Images/ab52939-65456-anti-ras-antibody-ep1125y-flow-cytometry.gif)
Flow Cytometry (Intracellular) - Anti-Ras antibody [EP1125Y] (ab52939)Unpurified ab52939 at 1/100 dilution staining Ras in permeabilized PC-12 (rat adrenal gland pheochromocytoma cell line) cells by intracellular flow cytometry (red). Rabbit IgG negative control (green).
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![Western blot - Anti-Ras antibody [EP1125Y] (ab52939)](https://www.abcam.com/ps/products/52/ab52939/Images/ab52939-65455-anti-ras-antibody-ep1125y-western-blot.jpg)
Western blot - Anti-Ras antibody [EP1125Y] (ab52939)Anti-Ras antibody [EP1125Y] (ab52939) at 1/500 dilution (unpurified) + Human Ras full length protein (ab56526) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Exposure time: 1 minute -
![Anti-Ras antibody [EP1125Y] (ab52939)](https://www.abcam.com/ps/products/52/ab52939/Images/ab52939-11-benefits-of-recombinant-antibodies.png)
Anti-Ras antibody [EP1125Y] (ab52939)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (53)
ab52939 has been referenced in 53 publications.
- Zhao Z et al. Sophoridine suppresses lenvatinib-resistant hepatocellular carcinoma growth by inhibiting RAS/MEK/ERK axis via decreasing VEGFR2 expression. J Cell Mol Med 25:549-560 (2021). PubMed: 33210432
- Sun X et al. Exosome-transmitted miRNA-335-5p promotes colorectal cancer invasion and metastasis by facilitating EMT via targeting RASA1. Mol Ther Nucleic Acids 24:164-174 (2021). PubMed: 33767913
- Liu Q et al. Minocycline alleviates the symptoms of morphine withdrawal via the CaMKII-Ras-ERK signaling pathway. Neurosci Lett 752:135825 (2021). PubMed: 33727130
- Chen X et al. Incaspitolide A extracted from Carpesium cernuum induces apoptosis in vitro via the PI3K/AKT pathway in benign prostatic hyperplasia. Biosci Rep 41:N/A (2021). PubMed: 34100062
- Finn KJ et al. A Single-Step, High-Dose Selection Scheme Reveals Distinct Mechanisms of Acquired Resistance to Oncogenic Kinase Inhibition in Cancer Cells. Cancer Res 80:79-90 (2020). PubMed: 31641034