Recombinant Anti-Ras antibody [EPR3255] (ab108602)
![Western blot - Anti-Ras antibody [EPR3255] (ab108602)](https://www.abcam.com/ps/products/108/ab108602/Images/ab108602-351353-anti-ras-antibody-epr3255-westernblot-human-mouse-rat.jpg "Western blot - Anti-Ras antibody [EPR3255] (ab108602)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3255] to Ras
- Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Ras antibody [EPR3255]
See all Ras primary antibodies -
Description
Rabbit monoclonal [EPR3255] to Ras -
Host species
Rabbit -
Specificity
ab108602 also detects H-Ras and N-Ras. -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 293T and SH-SY5Y cell lysates; Rat brain lysates; Mouse heart lysates IP: SH-SY5Y cell lysates Flow Cyt (intra): HEK-293 cells ICC/IF: HEK-293T cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3255 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/30.
|
|
| WB | (6) |
1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
|
| IP | (2) |
1/10 - 1/100.
|
| ICC/IF |
1/50 - 1/100.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/30. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 22 kDa. |
|
IP
1/10 - 1/100. |
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ICC/IF
1/50 - 1/100. |
Target
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Function
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. -
Involvement in disease
Defects in HRAS are the cause of faciocutaneoskeletal syndrome (FCSS) [MIM:218040]. A rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities.
Defects in HRAS are the cause of congenital myopathy with excess of muscle spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome.
Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
Note=Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors.
Defects in HRAS are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.
Note=Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC). -
Sequence similarities
Belongs to the small GTPase superfamily. Ras family. -
Post-translational
modificationsPalmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation. -
Cellular localization
Cell membrane. Golgi apparatus membrane. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 3265 Human
- Entrez Gene: 3845 Human
- Entrez Gene: 4893 Human
- Entrez Gene: 15461 Mouse
- Entrez Gene: 16653 Mouse
- Entrez Gene: 18176 Mouse
- Entrez Gene: 24525 Rat
- Entrez Gene: 24605 Rat
see all -
Alternative names
- C BAS/HAS antibody
- C HA RAS1 antibody
- C-BAS/HAS antibody
see all
Images
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Western blot - Anti-Ras antibody [EPR3255] (ab108602)All lanes : Anti-Ras antibody [EPR3255] (ab108602) at 1/10000 dilution (Purified)
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : Rat brain lysates
Lane 3 : Mouse heart lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-Ras antibody [EPR3255] (ab108602)](https://www.abcam.com/ps/products/108/ab108602/Images/ab108602-4-anti-ras-antibody-epr3255-immunoprecipitation-shsy5y-human.jpg)
Immunoprecipitation - Anti-Ras antibody [EPR3255] (ab108602)ab108602 (purified) at 1/20 dilution (2 µg) immunoprecipitating Ras in SH-SY5Y whole cell lysate.
Lane 1 (input): SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10 µg
Lane 2 (+): ab108602 & SH-SY5Y whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108602 in SH-SY5Y whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Flow Cytometry (Intracellular) - Anti-Ras antibody [EPR3255] (ab108602)](https://www.abcam.com/ps/products/108/ab108602/Images/ab108602-3-anti-ras-antibody-epr3255-flowcytometry-hek293-human.jpg)
Flow Cytometry (Intracellular) - Anti-Ras antibody [EPR3255] (ab108602)Intracellular Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling Ras with purified ab108602 at 1/30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EPR3255] (ab108602)](https://www.abcam.com/ps/products/108/ab108602/Images/ab108602-2-anti-ras-antibody-epr3255-immunocytochemistry-293t-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EPR3255] (ab108602)Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling Ras with purified ab108602 at 1:100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Western blot - Anti-Ras antibody [EPR3255] (ab108602)](https://www.abcam.com/ps/products/108/ab108602/Images/ab108602-121318-anti-ras-antibody-epr3255-western-blot.jpg)
Western blot - Anti-Ras antibody [EPR3255] (ab108602)All lanes : Anti-Ras antibody [EPR3255] (ab108602) at 1/1000 dilution (unpurified)
Lane 1 : HEK-293 whole cell lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 22 kDa -
![Anti-Ras antibody [EPR3255] (ab108602)](https://www.abcam.com/ps/products/108/ab108602/Images/ab108602-6-benefits-of-recombinant-antibodies.png)
Anti-Ras antibody [EPR3255] (ab108602)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab108602 has been referenced in 19 publications.
- Xie H et al. Remimazolam alleviates neuropathic pain via regulating bradykinin receptor B1 and autophagy. J Pharm Pharmacol N/A:N/A (2021). PubMed: 34061162
- Pandit M et al. Geranylgeranyl pyrophosphate amplifies Treg differentiation via increased IL-2 expression to ameliorate DSS-induced colitis. Eur J Immunol 51:1461-1472 (2021). PubMed: 33548071
- Posternak G et al. Functional characterization of a PROTAC directed against BRAF mutant V600E. Nat Chem Biol 16:1170-1178 (2020). PubMed: 32778845
- Chen Z et al. Geniposide exhibits anticancer activity to medulloblastoma cells by downregulating microRNA-373. J Biochem Mol Toxicol 34:e22471 (2020). PubMed: 32057176
- Cruz VH et al. Axl-mediated activation of TBK1 drives epithelial plasticity in pancreatic cancer. JCI Insight 5:N/A (2019). PubMed: 30938713