Recombinant Anti-RASSF2 antibody [EPR6621] (ab124786)
![Western blot - Anti-RASSF2 antibody [EPR6621] (ab124786)](https://www.abcam.com/ps/products/124/ab124786/Images/ab124786-138667-anti-rassf2-antibody-epr6621-western-blot.jpg "Western blot - Anti-RASSF2 antibody [EPR6621] (ab124786)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6621] to RASSF2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RASSF2 antibody [EPR6621]
See all RASSF2 primary antibodies -
Description
Rabbit monoclonal [EPR6621] to RASSF2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human RASSF2 aa 300-400 (C terminal). The exact sequence is proprietary.
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Positive control
- Raji, Jurkat and SH-SY5Y cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.80 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6621 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
Target
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Function
Potential tumor suppressor. Acts as a KRAS-specific effector protein. May promote apoptosis and cell cycle arrest. -
Tissue specificity
Widely expressed with highest levels in brain, placenta, peripheral blood and lung. Frequently down-regulated in lung tumor cell lines. -
Sequence similarities
Contains 1 Ras-associating domain.
Contains 1 SARAH domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9770 Human
- Omim: 609492 Human
- SwissProt: P50749 Human
- Unigene: 631504 Human
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Alternative names
- CENP 34 antibody
- Centromere protein 34 antibody
- Ras association (RalGDS/AF 6) domain family 2 antibody
see all
Images
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Western blot - Anti-RASSF2 antibody [EPR6621] (ab124786)All lanes : Anti-RASSF2 antibody [EPR6621] (ab124786) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa -
![OI-RD Scanning - Anti-RASSF2 antibody [EPR6621] (ab124786)](https://www.abcam.com/ps/products/124/ab124786/Images/ab124786-138666-anti-rassf2-antibody-epr6621.jpg)
OI-RD Scanning - Anti-RASSF2 antibody [EPR6621] (ab124786) -
![Anti-RASSF2 antibody [EPR6621] (ab124786)](https://www.abcam.com/ps/products/124/ab124786/Images/ab124786-3-benefits-of-recombinant-antibodies.png)
Anti-RASSF2 antibody [EPR6621] (ab124786)
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab124786 has been referenced in 1 publication.
- Guerrero-Setas D et al. RASSF2 hypermethylation is present and related to shorter survival in squamous cervical cancer. Mod Pathol N/A:N/A (2013). IHC ; Human . PubMed: 23542458