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Rat IgM, kappa monoclonal [RTK2118] - Isotype Control (ab35768)

Q&A (1)References (2)
Flow Cytometry - Rat IgM, kappa monoclonal [RTK2118] - Isotype Control (ab35768)

    Conjugates logo Related conjugates and formulations

    Biotin FITC

    Overview

    • Product name

      Rat IgM, kappa monoclonal [RTK2118] - Isotype Control
      See all Rat isotype controls

    • Tested applications

      Suitable for: Flow Cyt, WB, IP, ICC/IF, IHC-Fr, IHC-Pmore details

    • Immunogen

      Chemical/ Small Molecule corresponding to Rat. Trinitrophenol + KLH

    • General notes


      Isotype Control monoclonal antibodies are used to estimate the non-specific binding of target primary antibodies to cell surface antigens. Non-specific binding is due to Fc receptor binding or other protein-protein interactions. Isotype controls show negligible cross-reactivity with cell surface antigens on tissue sections or in cellular preparations. Isotype Controls should be used at identical concentrations and staining conditions as the target primary antibodies.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • Purity

      Purified IgM

    • Isotype control notes

      Isotype Control monoclonal antibodies are used to estimate the non-specific binding of target primary antibodies to cell surface antigens. Non-specific binding is due to Fc receptor binding or other protein-protein interactions. Isotype controls show negligible cross-reactivity with cell surface antigens on tissue sections or in cellular preparations. Isotype Controls should be used at identical concentrations and staining conditions as the target primary antibodies.

    • Clonality

      Monoclonal

    • Clone number

      RTK2118

    • Isotype

      IgM

    • Light chain type

      kappa

    • Research areas

    • Alternative names

      • Rat Isotype Control

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab35768 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 2µg for 106 cells.

    ab35768 is a negative isotype control 
    WB
    Use at an assay dependent concentration.
    IP
    Use at an assay dependent concentration.
    ICC/IF
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    Notes
    Flow Cyt
    Use 2µg for 106 cells.

    ab35768 is a negative isotype control 
    WB
    Use at an assay dependent concentration.
    IP
    Use at an assay dependent concentration.
    ICC/IF
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.

    Images

    • Flow Cytometry - Rat IgM, kappa monoclonal [RTK2118] - Isotype Control (ab35768)
      Flow Cytometry - Rat IgM, kappa monoclonal [RTK2118] - Isotype Control (ab35768)

      Overlay histogram showing RAMOS cells stained with ab19795 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19795, 1/10 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat kappa monoclonal [RTK2118] (ab35768, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    References (2)

    Publishing research using ab35768? Please let us know so that we can cite the reference in this datasheet.

    ab35768 has been referenced in 2 publications.

    • Chandrupatla DMSH  et al. Imaging and Methotrexate Response Monitoring of Systemic Inflammation in Arthritic Rats Employing the Macrophage PET Tracer [18F]Fluoro-PEG-Folate. Contrast Media Mol Imaging 2018:8092781 (2018). PubMed: 29681783
    • Pereira EM  et al. Characterization and phosphoproteomic analysis of a human immortalized podocyte model of Fabry disease generated using CRISPR/Cas9 technology. Am J Physiol Renal Physiol 311:F1015-F1024 (2016). PubMed: 27681560

    Customer reviews and Q&As

    Q&A
    Submit a question

    Question

    Provide the discount code for ab18407 and ab18541

    Read More
    Answer

    Expiration date: 25-04-2012
    I am very pleased to hear you would like to use ab18407 and ab18541 in ICC/IF and send us an image of your results.
    This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, please submit an Abreview with the image for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
    The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways:
    1) Call to place your order and mention the code to our customer service department;
    2) Include the code in your fax order;
    3) Place your order on the web and enter the promotional code.
    Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.
    If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com