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    products/primary-antibodies/rbbp4-antibody-epr3412-chip-grade-ab92344.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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RecombinantRabMAb

Recombinant Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (3)

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ChIP-sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)
  • Western blot - Anti-RBBP4 antibody [EPR3412] (ab92344)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBBP4 antibody [EPR3412] (ab92344)
  • Anti-RBBP4 antibody [EPR3412] (ab92344)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3412] to RBBP4 - ChIP Grade
  • Suitable for: WB, IHC-P, ChIP-sequencing
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Protein
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Recombinant Human RBBP4 protein (ab130037)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Isotype
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Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)

View more associated products

Overview

  • Product name

    Anti-RBBP4 antibody [EPR3412] - ChIP Grade
    See all RBBP4 primary antibodies
  • Description

    Rabbit monoclonal [EPR3412] to RBBP4 - ChIP Grade
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ChIP-sequencingmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human RBBP4 aa 400 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • WB and IHC: HeLa, Jurkat, Raji and 293T cell lysates; Human breast carcinoma tissue. ChIP-Seq: Chromatin from HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3412
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • ISWI/NURF/CHRAC
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Polycomb Silencing
    • PRC2
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Rb
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class I
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Rb family
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

Associated products

  • Alternative Versions

    • Anti-RBBP4 antibody [EPR3412] - ChIP Grade – BSA and Azide free (ab247547)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
  • Recombinant Protein

    • Recombinant Human RBBP4 protein (ab130037)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/20000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIP-sequencing
Use 8µg for 107 cells.
Notes
WB
1/10000 - 1/20000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIP-sequencing
Use 8µg for 107 cells.

Target

  • Function

    Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
  • Sequence similarities

    Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
    Contains 6 WD repeats.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q09028 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5928 Human
    • Entrez Gene: 19646 Mouse
    • Omim: 602923 Human
    • SwissProt: Q09028 Human
    • SwissProt: Q60972 Mouse
    • Unigene: 16003 Human
    • Unigene: 12145 Mouse
    • Alternative names

      • CAF I p48 antibody
      • CAF-1 subunit C antibody
      • CAF-I 48 kDa subunit antibody
      • CAF-I p48 antibody
      • Chromatin assembly factor 1 subunit C antibody
      • Chromatin assembly factor I p48 subunit antibody
      • Chromatin assembly factor/CAF 1 p48 subunit antibody
      • Histone-binding protein RBBP4 antibody
      • MSI1 protein homolog antibody
      • Nucleosome-remodeling factor subunit RBAP48 antibody
      • NURF55 antibody
      • RB binding protein 4 chromatin remodeling factor antibody
      • RbAp 48 antibody
      • RBAP48 antibody
      • RBBP-4 antibody
      • RBBP4 antibody
      • RBBP4_HUMAN antibody
      • Retinoblastoma binding protein 4 antibody
      • Retinoblastoma binding protein p48 antibody
      • Retinoblastoma-binding protein 4 antibody
      • Retinoblastoma-binding protein p48 antibody
      see all

    Images

    • ChIP-sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)
      ChIP-sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)

      Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 HeLa cells and 8 µg of ab92344 [EPR3412]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.

      Additional screenshots of mapped reads can be downloaded here.

    • Western blot - Anti-RBBP4 antibody [EPR3412] (ab92344)
      Western blot - Anti-RBBP4 antibody [EPR3412] (ab92344)
      All lanes : Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344) at 1/20000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Raji
      cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBBP4 antibody [EPR3412] (ab92344)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBBP4 antibody [EPR3412] (ab92344)

      ab92344 at 1/250 dilution staining RBBP4 in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.

      Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

    • Anti-RBBP4 antibody [EPR3412] (ab92344)
      Anti-RBBP4 antibody [EPR3412] (ab92344)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab92344? Please let us know so that we can cite the reference in this datasheet.

    ab92344 has been referenced in 3 publications.

    • Li YD  et al. RBBP4 promotes colon cancer malignant progression via regulating Wnt/ß-catenin pathway. World J Gastroenterol 26:5328-5342 (2020). PubMed: 32994691
    • Akiyama T  et al. Transient bursts of Zscan4 expression are accompanied by the rapid derepression of heterochromatin in mouse embryonic stem cells. DNA Res 22:307-18 (2015). PubMed: 26324425
    • Davidovich C  et al. Promiscuous RNA binding by Polycomb repressive complex 2. Nat Struct Mol Biol 20:1250-7 (2013). PubMed: 24077223

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RbAp48 antibody [EPR3412]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kirk Mcmanus

    Verified customer

    Submitted Nov 02 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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