Recombinant Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)
![ChIP-sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)](https://www.abcam.com/ps/products/92/ab92344/Images/ab92344-479199-anti-rbbp4-antibody-epr3412-chip-grade-chip-sequencing.jpg "ChIP-sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3412] to RBBP4 - ChIP Grade
- Suitable for: WB, IHC-P, ChIP-sequencing
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RBBP4 antibody [EPR3412] - ChIP Grade
See all RBBP4 primary antibodies -
Description
Rabbit monoclonal [EPR3412] to RBBP4 - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ChIP-sequencingmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human RBBP4 aa 400 to the C-terminus. The exact sequence is proprietary.
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Positive control
- WB and IHC: HeLa, Jurkat, Raji and 293T cell lysates; Human breast carcinoma tissue. ChIP-Seq: Chromatin from HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3412 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/20000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ChIP-sequencing |
Use 8µg for 107 cells.
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| Notes |
|---|
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WB
1/10000 - 1/20000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ChIP-sequencing
Use 8µg for 107 cells. |
Target
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Function
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex. -
Sequence similarities
Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
Contains 6 WD repeats. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5928 Human
- Entrez Gene: 19646 Mouse
- Omim: 602923 Human
- SwissProt: Q09028 Human
- SwissProt: Q60972 Mouse
- Unigene: 16003 Human
- Unigene: 12145 Mouse
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Alternative names
- CAF I p48 antibody
- CAF-1 subunit C antibody
- CAF-I 48 kDa subunit antibody
see all
Images
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ChIP-sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)
Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 HeLa cells and 8 µg of ab92344 [EPR3412]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.
Additional screenshots of mapped reads can be downloaded here.
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![Western blot - Anti-RBBP4 antibody [EPR3412] (ab92344)](https://www.abcam.com/ps/products/92/ab92344/Images/ab92344-107324-anti-rbap48-antibody-epr3412-western-blot.jpg)
Western blot - Anti-RBBP4 antibody [EPR3412] (ab92344)All lanes : Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344) at 1/20000 dilution
Lane 1 : HeLa lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Raji
cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBBP4 antibody [EPR3412] (ab92344)](https://www.abcam.com/ps/products/92/ab92344/Images/ab92344-107323-anti-rbap48-antibody-epr3412-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBBP4 antibody [EPR3412] (ab92344)ab92344 at 1/250 dilution staining RBBP4 in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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![Anti-RBBP4 antibody [EPR3412] (ab92344)](https://www.abcam.com/ps/products/92/ab92344/Images/ab92344-3-benefits-of-recombinant-antibodies.png)
Anti-RBBP4 antibody [EPR3412] (ab92344)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab92344 has been referenced in 3 publications.
- Li YD et al. RBBP4 promotes colon cancer malignant progression via regulating Wnt/ß-catenin pathway. World J Gastroenterol 26:5328-5342 (2020). PubMed: 32994691
- Akiyama T et al. Transient bursts of Zscan4 expression are accompanied by the rapid derepression of heterochromatin in mouse embryonic stem cells. DNA Res 22:307-18 (2015). PubMed: 26324425
- Davidovich C et al. Promiscuous RNA binding by Polycomb repressive complex 2. Nat Struct Mol Biol 20:1250-7 (2013). PubMed: 24077223