Recombinant Anti-RBPJK antibody [7A11] (ab252858)
![Western blot - Anti-RBPJK antibody [7A11] (ab252858)](https://www.abcam.com/ps/products/252/ab252858/Images/ab252858-387086-AntiRBPJK-antibody-7a11-wb-various-cells.jpg "Western blot - Anti-RBPJK antibody [7A11] (ab252858)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [7A11] to RBPJK
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-RBPJK antibody [7A11]
See all RBPJK primary antibodies -
Description
Rat monoclonal [7A11] to RBPJK -
Host species
Rat -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Daudi, Raji, MCF7, HeLa C6, RAW264.7, NIH/3T3 whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
7A11 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 56 kDa.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 56 kDa. |
Target
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Function
Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. -
Sequence similarities
Belongs to the Su(H) family.
Contains 1 IPT/TIG domain. -
Cellular localization
Nucleus. Cytoplasm. Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway. - Information by UniProt
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Database links
- Entrez Gene: 3516 Human
- Entrez Gene: 19664 Mouse
- Entrez Gene: 679028 Rat
- Omim: 147183 Human
- SwissProt: Q06330 Human
- SwissProt: P31266 Mouse
- Unigene: 479396 Human
- Unigene: 209292 Mouse
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Alternative names
- AI843960 antibody
- AOS3 antibody
- CBF 1 antibody
see all
Images
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Western blot - Anti-RBPJK antibody [7A11] (ab252858)All lanes : Anti-RBPJK antibody [7A11] (ab252858) at 1/1000 dilution
Lane 1 : Daudi (human Burkitt’s lymphoma lymphoblast), whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 56 kDaBlocking buffer: 5% NFDM/TBST.
Exposur time:
lane 1, 2: 180 seconds
lane 3, 4: 48 secondsThe molecular weight observed was in consistent with the literature (PMID:25512468) and the benchmark ab180588.
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![Western blot - Anti-RBPJK antibody [7A11] (ab252858)](https://www.abcam.com/ps/products/252/ab252858/Images/ab252858-387085-AntiRBPJK-antibody-7a11-wb-various-mouse-and-rat--cells.jpg)
Western blot - Anti-RBPJK antibody [7A11] (ab252858)All lanes : Anti-RBPJK antibody [7A11] (ab252858) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking buffer: 5% NFDM/TBST.
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![Anti-RBPJK antibody [7A11] (ab252858)](https://www.abcam.com/ps/products/252/ab252858/Images/ab252858-3-benefits-of-recombinant-antibodies.png)
Anti-RBPJK antibody [7A11] (ab252858)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab252858 has not yet been referenced specifically in any publications.