Recombinant Anti-RBPJK antibody [EPR13479] (ab180588)
![Western blot - Anti-RBPJK antibody [EPR13479] (ab180588)](https://www.abcam.com/ps/products/180/ab180588/Images/ab180588-330561-anti-rbpjk-antibody-epr13479-western-blot.jpg "Western blot - Anti-RBPJK antibody [EPR13479] (ab180588)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13479] to RBPJK
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-RBPJK antibody [EPR13479]
See all RBPJK primary antibodies -
Description
Rabbit monoclonal [EPR13479] to RBPJK -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 293 cells, MCF7, Raji, F9, C9, and HeLa cell line lysates; ICC/IF: 293T cells; Flow Cyt (intra): HEK-293;
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13479 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
|
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| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).
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| ICC/IF | (1) |
1/100.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/100. |
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WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa). |
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ICC/IF
1/100. |
Target
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Function
Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. -
Sequence similarities
Belongs to the Su(H) family.
Contains 1 IPT/TIG domain. -
Cellular localization
Nucleus. Cytoplasm. Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway. - Information by UniProt
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Database links
- Entrez Gene: 3516 Human
- Entrez Gene: 19664 Mouse
- Entrez Gene: 679028 Rat
- Omim: 147183 Human
- SwissProt: Q06330 Human
- SwissProt: P31266 Mouse
- Unigene: 479396 Human
- Unigene: 209292 Mouse
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Alternative names
- AI843960 antibody
- AOS3 antibody
- CBF 1 antibody
see all
Images
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Western blot - Anti-RBPJK antibody [EPR13479] (ab180588)All lanes : Anti-RBPJK antibody [EPR13479] (ab180588) at 1/1000 dilution (Purified)
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 15 µg
Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 3 : F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysates at 15 µg
Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56-60 kDa why is the actual band size different from the predicted?The double bands are consistent with what has been described in the literature PMID: 9102301 and 24603501
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![Immunocytochemistry/ Immunofluorescence - Anti-RBPJK antibody [EPR13479] (ab180588)](https://www.abcam.com/ps/products/180/ab180588/Images/ab180588-330490-anti-rbpjk-antibody-epr13479-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RBPJK antibody [EPR13479] (ab180588)Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling RBPJK with Purified ab180588 at 1:100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Flow Cytometry (Intracellular) - Anti-RBPJK antibody [EPR13479] (ab180588)](https://www.abcam.com/ps/products/180/ab180588/Images/ab180588-330483-anti-rbpjk-antibody-epr13479-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-RBPJK antibody [EPR13479] (ab180588)Intracellular Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling RBPJK with Purified ab180588 at 1/100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Western blot - Anti-RBPJK antibody [EPR13479] (ab180588)](https://www.abcam.com/ps/products/180/ab180588/Images/ab180588-317214-anti-rbpjk-antibody-epr13479-western-blot.jpg)
Western blot - Anti-RBPJK antibody [EPR13479] (ab180588)All lanes : Anti-RBPJK antibody [EPR13479] (ab180588) at 0.4 µg/ml (Unpurified)
Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 4 : Raw264.7 (Mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 56 kDaBlocking and diluting buffer: 5% NFDM/TBST.
Exposure time: Lane 1-3: 3 minutes
Lane 4: 45 seconds -
![Immunocytochemistry/ Immunofluorescence - Anti-RBPJK antibody [EPR13479] (ab180588)](https://www.abcam.com/ps/products/180/ab180588/Images/ab180588-211274-anti-rbpjk-antibody-epr13479-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RBPJK antibody [EPR13479] (ab180588)Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling RBPJK with unpurified ab180588 at 1/100 (green). Dapi shown in blue.
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![Western blot - Anti-RBPJK antibody [EPR13479] (ab180588)](https://www.abcam.com/ps/products/180/ab180588/Images/ab180588-318830-anti-rbpjk-antibody-epr13479-western-blot.jpg)
Western blot - Anti-RBPJK antibody [EPR13479] (ab180588)All lanes : Anti-RBPJK antibody [EPR13479] (ab180588) at 1/1000 dilution (Unpurified)
Lane 1 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 20ug prepared using RIPA lysis method
Lane 2 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 20ug prepared using 1% SDS hot lysis method
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Diluting buffer and concentration 5% NFDM/TBST.
Extra band at: 90kDa
We are unsure as to the identity of this extra band.For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
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![Western blot - Anti-RBPJK antibody [EPR13479] (ab180588)](https://www.abcam.com/ps/products/180/ab180588/Images/ab180588-211272-anti-rbpjk-antibody-epr13479-western-blot.jpg)
Western blot - Anti-RBPJK antibody [EPR13479] (ab180588)All lanes : Anti-RBPJK antibody [EPR13479] (ab180588) at 1/2000 dilution (Unpurified)
Lane 1 : 293 cell line lysate
Lane 2 : MCF7 cell line lysate
Lane 3 : HeLa cell line lysate
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
![Anti-RBPJK antibody [EPR13479] (ab180588)](https://www.abcam.com/ps/products/180/ab180588/Images/ab180588-8-benefits-of-recombinant-antibodies.png)
Anti-RBPJK antibody [EPR13479] (ab180588)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (15)
ab180588 has been referenced in 15 publications.
- He WZ et al. miR-188-3p targets skeletal endothelium coupling of angiogenesis and osteogenesis during ageing. Cell Death Dis 13:494 (2022). PubMed: 35610206
- Zhang XLN et al. Gastrodin exerts protective effects in reactive TNC1 astrocytes via regulation of the Notch signaling pathway. Ann Transl Med 9:1754 (2021). PubMed: 35071448
- Luo Z et al. Silencing of RBP-JK promotes the differentiation of bone marrow mesenchymal stem cells into vascular endothelial cells. Mol Med Rep 21:69-76 (2020). PubMed: 31746399
- Zhang X et al. ID4 Promotes Breast Cancer Chemotherapy Resistance via CBF1-MRP1 Pathway. J Cancer 11:3846-3857 (2020). PubMed: 32328189
- Chia W et al. A circular RNA derived from DAB1 promotes cell proliferation and osteogenic differentiation of BMSCs via RBPJ/DAB1 axis. Cell Death Dis 11:372 (2020). PubMed: 32415085