Recombinant Anti-REA antibody [EPR14522] (ab181838)
![Western blot - Anti-REA antibody [EPR14522] (ab181838)](https://www.abcam.com/ps/products/181/ab181838/Images/ab181838-212735-ab181838WB.jpg "Western blot - Anti-REA antibody [EPR14522] (ab181838)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14522] to REA
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-REA antibody [EPR14522]
See all REA primary antibodies -
Description
Rabbit monoclonal [EPR14522] to REA -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, 293T and Human fetal kidney lysates; HeLa and 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14522 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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| ICC/IF |
1/250.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
|
ICC/IF
1/250. |
Target
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Function
Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity). Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging. -
Sequence similarities
Belongs to the prohibitin family. -
Developmental stage
Levels of expression in fibroblasts decrease heterogeneously during cellular aging. -
Cellular localization
Mitochondrion inner membrane. Cytoplasm. Nucleus. Also cytoplasmic and nuclear. - Information by UniProt
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Database links
- Entrez Gene: 11331 Human
- Entrez Gene: 12034 Mouse
- Entrez Gene: 114766 Rat
- Omim: 610704 Human
- SwissProt: Q99623 Human
- SwissProt: O35129 Mouse
- SwissProt: Q5XIH7 Rat
- Unigene: 504620 Human
see all -
Alternative names
- B cell receptor associated protein BAP37 antibody
- B-cell receptor-associated protein BAP37 antibody
- BAP antibody
see all
Images
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Western blot - Anti-REA antibody [EPR14522] (ab181838)Anti-REA antibody [EPR14522] (ab181838) at 1/10000 dilution + HeLa cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa -
![Western blot - Anti-REA antibody [EPR14522] (ab181838)](https://www.abcam.com/ps/products/181/ab181838/Images/ab181838-212733-ab181838WBb.jpg)
Western blot - Anti-REA antibody [EPR14522] (ab181838)All lanes : Anti-REA antibody [EPR14522] (ab181838) at 1/10000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-REA antibody [EPR14522] (ab181838)](https://www.abcam.com/ps/products/181/ab181838/Images/ab181838-212732-ab181838IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-REA antibody [EPR14522] (ab181838)Immunofluorescent analysis of acetone-fixed HeLa cells labeling REA with ab181838 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-REA antibody [EPR14522] (ab181838)](https://www.abcam.com/ps/products/181/ab181838/Images/ab181838-212731-ab181838IFb.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-REA antibody [EPR14522] (ab181838)Immunofluorescent analysis of acetone-fixed 293T cells labeling REA with ab181838 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.
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![Anti-REA antibody [EPR14522] (ab181838)](https://www.abcam.com/ps/products/181/ab181838/Images/ab181838-5-benefits-of-recombinant-antibodies.png)
Anti-REA antibody [EPR14522] (ab181838)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab181838 has been referenced in 2 publications.
- Yasukawa K et al. The microRNAs miR-302b and miR-372 regulate mitochondrial metabolism via the SLC25A12 transporter, which controls MAVS-mediated antiviral innate immunity. J Biol Chem 295:444-457 (2020). PubMed: 31767682
- Yoshinaka T et al. Structural Basis of Mitochondrial Scaffolds by Prohibitin Complexes: Insight into a Role of the Coiled-Coil Region. iScience 19:1065-1078 (2019). PubMed: 31522117