Recombinant Anti-REA antibody [EPR14523] (ab182139)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REA antibody [EPR14523] (ab182139)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14523] to REA
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-REA antibody [EPR14523]
See all REA primary antibodies
Description

Rabbit monoclonal [EPR14523] to REA
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HT-1080, 293T, Mouse brain, RAW 264.7, and PC-12 lysates. IHC-P: human breast, mouse kidney, and rat kidney tissues. ICC/IF: HeLa and 293T cells. Flow Cyt (intra): HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14523
Isotype

IgG
Research areas

Alternative Versions
- Anti-REA antibody [EPR14523] - BSA and Azide free (ab250582)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HeLa whole cell lysate (ab150035)
- HeLa whole cell lysate (ab29545)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab182139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/70.
WB		1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
IHC-P		1/8000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified use at 1/100 - 1/250. See IHC antigen retrieval protocols
ICC/IF		1/50. For unpurified use at 1/100 - 1/250.

Notes
Flow Cyt (Intra) 1/70.
WB 1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
IHC-P 1/8000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified use at 1/100 - 1/250. See IHC antigen retrieval protocols
ICC/IF 1/50. For unpurified use at 1/100 - 1/250.

Function

Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity). Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging.
Sequence similarities

Belongs to the prohibitin family.
Developmental stage

Levels of expression in fibroblasts decrease heterogeneously during cellular aging.
Cellular localization

Mitochondrion inner membrane. Cytoplasm. Nucleus. Also cytoplasmic and nuclear.
Target information above from: UniProt accession Q99623 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 11331 Human
- Entrez Gene: 12034 Mouse
- Entrez Gene: 114766 Rat
- Omim: 610704 Human
- SwissProt: Q99623 Human
- SwissProt: O35129 Mouse
- SwissProt: Q5XIH7 Rat
- Unigene: 504620 Human
- Unigene: 36241 Mouse
- Unigene: 64535 Rat
see all
Alternative names
- B cell receptor associated protein BAP37 antibody
- B-cell receptor-associated protein BAP37 antibody
- BAP antibody
- Bap37 antibody
- BCAP 37 antibody
- D prohibitin antibody
- D-prohibitin antibody
- p22 antibody
- Phb2 antibody
- PHB2_HUMAN antibody
- PNAS 141 antibody
- Prohibitin 2 antibody
- Prohibitin-2 antibody
- Repressor of estrogen receptor activity antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REA antibody [EPR14523] (ab182139)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling REA with purified ab182139 at 1:8000 dilution (0.09 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-REA antibody [EPR14523] (ab182139)

Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling REA with purified ab182139 at 1:50 dilution (10 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry (Intracellular) - Anti-REA antibody [EPR14523] (ab182139)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling REA with purified ab182139 at 1/70 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REA antibody [EPR14523] (ab182139)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling REA with purified ab182139 at 1:8000 dilution (0.09 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REA antibody [EPR14523] (ab182139)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling REA with purified ab182139 at 1:8000 dilution (0.09 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-REA antibody [EPR14523] (ab182139)

All lanes : Anti-REA antibody [EPR14523] (ab182139) at 1/10000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 33 kDa
Western blot - Anti-REA antibody [EPR14523] (ab182139)

All lanes : Anti-REA antibody [EPR14523] (ab182139) at 1/20000 dilution (Unpurified)

Lane 1 : HT-1080 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 33 kDa

Blocking/ Dilution buffer: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REA antibody [EPR14523] (ab182139)

Immunohistochemical analysis of paraffin embedded Human transitional cell carcinoma of bladder tissue sections labeling REA using unpurified ab182139 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REA antibody [EPR14523] (ab182139)

Immunohistochemical analysis of paraffin embedded mouse kidney tissue sections labeling REA using unpurified ab182139 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-REA antibody [EPR14523] (ab182139)

Immunofluorescent analysis of acetone fixed HeLa cells labeling REA using unpurified ab182139 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution. Counterstain DAPI.
Immunocytochemistry/ Immunofluorescence - Anti-REA antibody [EPR14523] (ab182139)

Immunofluorescent analysis of paraformaldehyde fixed 293T cells labeling REA using unpurified ab182139 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution. Counterstain DAPI.
Anti-REA antibody [EPR14523] (ab182139)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab182139? Please let us know so that we can cite the reference in this datasheet.

ab182139 has been referenced in 1 publication.

Wei X et al. Reducing NADPH Synthesis Counteracts Diabetic Nephropathy through Restoration of AMPK Activity in Type 1 Diabetic Rats. Cell Rep 32:108207 (2020). PubMed: 32997989

Customer reviews and Q&As

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Developmental stage

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (1)

Customer reviews and Q&As