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    products/primary-antibodies/rec8-antibody-epr16189-ab192241.pdf

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Cell Biology Cell Cycle Cell Division Chromatid Cohesion
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RecombinantRabMAb

Recombinant Anti-REC8 antibody [EPR16189] (ab192241)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (3) Submit a question References (6)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)
  • Western blot - Anti-REC8 antibody [EPR16189] (ab192241)
  • Western blot - Anti-REC8 antibody [EPR16189] (ab192241)
  • Anti-REC8 antibody [EPR16189] (ab192241)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16189] to REC8
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-REC8 antibody [EPR16189]
    See all REC8 primary antibodies
  • Description

    Rabbit monoclonal [EPR16189] to REC8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human, mouse and rat testis and Jurkat lysates; Human, mouse and rat testis tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16189
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Chromatid Cohesion

Associated products

  • Alternative Versions

    • Anti-REC8 antibody [EPR16189] - BSA and Azide free (ab245771)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse testis normal tissue lysate - total protein (ab29289)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab192241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Detects a band of approximately 85-95 kDa (predicted molecular weight: 67 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 85-95 kDa (predicted molecular weight: 67 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.
  • Tissue specificity

    Expressed in testis and thymus.
  • Sequence similarities

    Belongs to the rad21 family.
  • Post-translational
    modifications

    Phosphorylated.
  • Cellular localization

    Nucleus. Chromosome. In meiotic chromosomes, localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages, diakinesis and metaphase I, localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II.
  • Target information above from: UniProt accession O95072 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9985 Human
    • Entrez Gene: 56739 Mouse
    • Entrez Gene: 290227 Rat
    • Omim: 608193 Human
    • SwissProt: O95072 Human
    • SwissProt: Q8C5S7 Mouse
    • SwissProt: Q6AYJ4 Rat
    • Unigene: 419259 Human
    • Unigene: 23149 Mouse
    • Unigene: 108612 Rat
    see all
  • Alternative names

    • Cohesin Rec8p antibody
    • Human homolog of rad21 S. pombe antibody
    • Meiotic recombination and sister chromatid cohesion phosphoprotein of the rad21p family antibody
    • Meiotic recombination protein REC8 homolog antibody
    • Meiotic recombination protein REC8-like 1 antibody
    • rec8 antibody
    • REC8 homolog (yeast) antibody
    • REC8 like 1 antibody
    • REC8_HUMAN antibody
    • Rec8p antibody
    • Recombination and sister chromatid cohesion protein homolog antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling REC8 with ab192241 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling REC8 with ab192241 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling REC8 with ab192241 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-REC8 antibody [EPR16189] (ab192241)
    Western blot - Anti-REC8 antibody [EPR16189] (ab192241)
    All lanes : Anti-REC8 antibody [EPR16189] (ab192241) at 1/10000 dilution

    Lane 1 : Mouse testis lysate
    Lane 2 : Rat testis lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 67 kDa

  • Western blot - Anti-REC8 antibody [EPR16189] (ab192241)
    Western blot - Anti-REC8 antibody [EPR16189] (ab192241)
    All lanes : Anti-REC8 antibody [EPR16189] (ab192241) at 1/1000 dilution

    Lane 1 : Human testis lysate
    Lane 2 : Jurkat lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 67 kDa

  • Anti-REC8 antibody [EPR16189] (ab192241)
    Anti-REC8 antibody [EPR16189] (ab192241)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (6)

Publishing research using ab192241? Please let us know so that we can cite the reference in this datasheet.

ab192241 has been referenced in 6 publications.

  • Hsieh CL  et al. MIWI prevents aneuploidy during meiosis by cleaving excess satellite RNA. EMBO J 39:e103614 (2020). PubMed: 32677148
  • Li Y  et al. Cyclin B3 is required for metaphase to anaphase transition in oocyte meiosis I. J Cell Biol 218:1553-1563 (2019). PubMed: 30770433
  • Zhao J  et al. REC8 inhibits EMT by downregulating EGR1 in gastric cancer cells. Oncol Rep 39:1583-1590 (2018). PubMed: 29393474
  • Castro W  et al. The transcription factor Rfx7 limits metabolism of NK cells and promotes their maintenance and immunity. Nat Immunol 19:809-820 (2018). PubMed: 29967452
  • Mu X  et al. Retinoic acid-induced CYP51 nuclear translocation promotes meiosis prophase I process and is correlated to the expression of REC8 and STAG3 in mice. Biol Open 7:N/A (2018). PubMed: 30420384
  • Suzuki A  et al. Loss of MAX results in meiotic entry in mouse embryonic and germline stem cells. Nat Commun 7:11056 (2016). PubMed: 27025988

Customer reviews and Q&As

Show All Reviews Q&A
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1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-REC8 antibody [EPR16189]

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T, Jurkat, SH-SY5Y)
Gel Running Conditions
Reduced Denaturing (gradient 4-20%)
Loading amount
40 µg
Treatment
proteasome or UBA1 inhibitor
Specification
HEK293T, Jurkat, SH-SY5Y
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 02 2023

Immunocytochemistry/ Immunofluorescence abreview for Anti-REC8 antibody [EPR16189]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (testis cell)
Permeabilization
No
Specification
testis cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 07 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-REC8 antibody [EPR16189]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (spermatocytes)
Permeabilization
No
Specification
spermatocytes
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 27°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 16 2016

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