Recombinant Anti-REC8 antibody [EPR16189] (ab192241)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)](https://www.abcam.com/ps/products/192/ab192241/Images/ab192241-230036-anti-rec8-antibody-epr16189-immunohistochemistry.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16189] to REC8
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-REC8 antibody [EPR16189]
See all REC8 primary antibodies -
Description
Rabbit monoclonal [EPR16189] to REC8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human, mouse and rat testis and Jurkat lysates; Human, mouse and rat testis tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16189 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192241 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 85-95 kDa (predicted molecular weight: 67 kDa).
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| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 85-95 kDa (predicted molecular weight: 67 kDa). |
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. -
Tissue specificity
Expressed in testis and thymus. -
Sequence similarities
Belongs to the rad21 family. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Nucleus. Chromosome. In meiotic chromosomes, localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages, diakinesis and metaphase I, localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II. - Information by UniProt
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Database links
- Entrez Gene: 9985 Human
- Entrez Gene: 56739 Mouse
- Entrez Gene: 290227 Rat
- Omim: 608193 Human
- SwissProt: O95072 Human
- SwissProt: Q8C5S7 Mouse
- SwissProt: Q6AYJ4 Rat
- Unigene: 419259 Human
see all -
Alternative names
- Cohesin Rec8p antibody
- Human homolog of rad21 S. pombe antibody
- Meiotic recombination and sister chromatid cohesion phosphoprotein of the rad21p family antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling REC8 with ab192241 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)](https://www.abcam.com/ps/products/192/ab192241/Images/ab192241-230035-anti-rec8-antibody-epr16189-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling REC8 with ab192241 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)](https://www.abcam.com/ps/products/192/ab192241/Images/ab192241-230034-anti-rec8-antibody-epr16189-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (ab192241)Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling REC8 with ab192241 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-REC8 antibody [EPR16189] (ab192241)](https://www.abcam.com/ps/products/192/ab192241/Images/ab192241-230033-anti-rec8-antibody-epr16189-western-blot.jpg)
Western blot - Anti-REC8 antibody [EPR16189] (ab192241)All lanes : Anti-REC8 antibody [EPR16189] (ab192241) at 1/10000 dilution
Lane 1 : Mouse testis lysate
Lane 2 : Rat testis lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 67 kDa -
![Western blot - Anti-REC8 antibody [EPR16189] (ab192241)](https://www.abcam.com/ps/products/192/ab192241/Images/ab192241-230032-anti-rec8-antibody-epr16189-western-blot.jpg)
Western blot - Anti-REC8 antibody [EPR16189] (ab192241)All lanes : Anti-REC8 antibody [EPR16189] (ab192241) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : Jurkat lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 67 kDa -
![Anti-REC8 antibody [EPR16189] (ab192241)](https://www.abcam.com/ps/products/192/ab192241/Images/ab192241-6-benefits-of-recombinant-antibodies.png)
Anti-REC8 antibody [EPR16189] (ab192241)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (10)
ab192241 has been referenced in 10 publications.
- Choi EH et al. Meiosis-specific cohesin complexes display essential and distinct roles in mitotic embryonic stem cell chromosomes. Genome Biol 23:70 (2022). PubMed: 35241136
- Koh YE et al. The Kleisin Subunits of Cohesin are Involved in the Fate Determination of Embryonic Stem Cells. Mol Cells 45:820-832 (2022). PubMed: 36172976
- Chatzidaki EE et al. Ovulation suppression protects against chromosomal abnormalities in mouse eggs at advanced maternal age. Curr Biol 31:4038-4051.e7 (2021). PubMed: 34314679
- Hsieh CL et al. MIWI prevents aneuploidy during meiosis by cleaving excess satellite RNA. EMBO J 39:e103614 (2020). PubMed: 32677148
- Li Y et al. Cyclin B3 is required for metaphase to anaphase transition in oocyte meiosis I. J Cell Biol 218:1553-1563 (2019). PubMed: 30770433
- Zhao J et al. REC8 inhibits EMT by downregulating EGR1 in gastric cancer cells. Oncol Rep 39:1583-1590 (2018). PubMed: 29393474
- Castro W et al. The transcription factor Rfx7 limits metabolism of NK cells and promotes their maintenance and immunity. Nat Immunol 19:809-820 (2018). PubMed: 29967452
- Mu X et al. Retinoic acid-induced CYP51 nuclear translocation promotes meiosis prophase I process and is correlated to the expression of REC8 and STAG3 in mice. Biol Open 7:N/A (2018). PubMed: 30420384
- Szydłowska A et al. Manipulating Cohesin Levels in Live Mouse Oocytes. Methods Mol Biol 1818:113-128 (2018). PubMed: 29961260
- Suzuki A et al. Loss of MAX results in meiotic entry in mouse embryonic and germline stem cells. Nat Commun 7:11056 (2016). PubMed: 27025988