Recombinant Anti-Renin antibody [EPR20693] (ab212197)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20693] to Renin
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Renin antibody [EPR20693]
See all Renin primary antibodies -
Description
Rabbit monoclonal [EPR20693] to Renin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Frmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human and mouse kidney tissues. IHC-Fr: Mouse and rat kidney tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20693 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (4) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
(for human); Use at 1/5000 dilution (for mouse). This antibody is not recommended for rat species in IHC application. In our hands this antibody showed non-specific staining on rat kidney tissue sections |
IHC-Fr |
1/6000.
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Notes |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. (for human); Use at 1/5000 dilution (for mouse). This antibody is not recommended for rat species in IHC application. In our hands this antibody showed non-specific staining on rat kidney tissue sections |
IHC-Fr
1/6000. |
Target
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Function
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. -
Involvement in disease
Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia. -
Sequence similarities
Belongs to the peptidase A1 family. -
Cellular localization
Secreted. Membrane. Associated to membranes via binding to ATP6AP2. - Information by UniProt
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Database links
- Entrez Gene: 5972 Human
- Entrez Gene: 19701 Mouse
- Entrez Gene: 19702 Mouse
- Entrez Gene: 24715 Rat
- Omim: 179820 Human
- SwissProt: P00797 Human
- SwissProt: P00796 Mouse
- SwissProt: P06281 Mouse
see all -
Alternative names
- Angiotensin forming enzyme antibody
- Angiotensin forming enzyme precursor antibody
- Angiotensinogenase antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (ab212197)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Renin with ab212197 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on juxtaglomerular cells of human kidney (PMID: 1898348). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval performed using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Renin with ab212197 at 1/6000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in juxtaglomerular cells of mouse kidney tissue section (PMID: 27080979).
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Heat mediated antigen retrieval performed using Tris-EDTA buffer (pH9.0) (ab94681).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (ab212197)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Renin with ab212197 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on juxtaglomerular cells of mouse kidney (PMID: 1898348). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval performed using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Renin with ab212197 at 1/6000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in juxtaglomerular cells of rat kidney tissue section (PMID: 27080979).
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Heat mediated antigen retrieval performed using Tris-EDTA buffer (pH9.0) (ab94681).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (8)
ab212197 has been referenced in 8 publications.
- Mochida Y et al. Piezo2 expression and its alteration by mechanical forces in mouse mesangial cells and renin-producing cells. Sci Rep 12:4197 (2022). PubMed: 35273307
- Minatoguchi S et al. A novel renal perivascular mesenchymal cell subset gives rise to fibroblasts distinct from classic myofibroblasts. Sci Rep 12:5389 (2022). PubMed: 35354870
- Vergara A et al. Enhanced Cardiorenal Protective Effects of Combining SGLT2 Inhibition, Endothelin Receptor Antagonism and RAS Blockade in Type 2 Diabetic Mice. Int J Mol Sci 23:N/A (2022). PubMed: 36361612
- Liu TH et al. Network and 16S rRNA Sequencing-Combined Approach Provides Insightal Evidence of Vitamin K2 for Salt-Sensitive Hypertension. Front Nutr 8:639467 (2021). PubMed: 33718421
- Neumann EK et al. Protocol for multimodal analysis of human kidney tissue by imaging mass spectrometry and CODEX multiplexed immunofluorescence. STAR Protoc 2:100747 (2021). PubMed: 34430920
- Watanabe H et al. Inhibition of the renin-angiotensin system causes concentric hypertrophy of renal arterioles in mice and humans. JCI Insight 6:N/A (2021). PubMed: 34762601
- DeLalio LJ et al. Pannexin 1 channels in renin-expressing cells influence renin secretion and blood pressure homeostasis. Kidney Int 98:630-644 (2020). PubMed: 32446934
- Kumar Gupta A et al. Asynchronous mixing of kidney progenitor cells potentiates nephrogenesis in organoids. Commun Biol 3:231 (2020). PubMed: 32393756