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    products/primary-antibodies/retinoic-acid-receptor-beta-antibody-epr2017-ab124701.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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RecombinantRabMAb

Recombinant Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (4)

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Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
  • Western blot - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
  • Western blot - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
  • OI-RD Scanning - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
  • Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2017] to Retinoic Acid Receptor beta
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

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Protein
Recombinant Human Retinoic Acid Receptor beta protein (ab82202)

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Overview

  • Product name

    Anti-Retinoic Acid Receptor beta antibody [EPR2017]
    See all Retinoic Acid Receptor beta primary antibodies
  • Description

    Rabbit monoclonal [EPR2017] to Retinoic Acid Receptor beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Daudi, MCF7, MDA-MB-435, and U-87 MG lysates. IHC-P: human bladder carcinoma and kidney tissues. ICC/IF: HCT 116 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.13 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2017
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Retinoic & Retinoid
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Retinoic & Retinoid
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Retanoic and retanoid

Associated products

  • Alternative Versions

    • HRP Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab198557)
    • Anti-Retinoic Acid Receptor beta antibody [EPR2017] - BSA and Azide free (ab247968)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • MCF7 nuclear extract lysate (ab14860)
  • Recombinant Protein

    • Recombinant Human Retinoic Acid Receptor beta protein (ab82202)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.
  • Involvement in disease

    Microphthalmia, syndromic, 12
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization

    Cytoplasm and Nucleus.
  • Target information above from: UniProt accession P10826 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5915 Human
    • Omim: 180220 Human
    • SwissProt: P10826 Human
    • Unigene: 543218 Human
    • Unigene: 581530 Human
    • Unigene: 654490 Human
    • Unigene: 733004 Human
    • Alternative names

      • HAP antibody
      • HBV-activated protein antibody
      • NR1B2 antibody
      • Nuclear receptor subfamily 1 group B member 2 antibody
      • RAR B antibody
      • RAR beta antibody
      • RAR epsilon antibody
      • RAR-beta antibody
      • RAR-epsilon antibody
      • RARB antibody
      • RARB_HUMAN antibody
      • Retinoic acid receptor beta 2 antibody
      • Retinoic acid receptor beta 4 antibody
      • Retinoic acid receptor beta 5 antibody
      • Retinoic acid receptor beta antibody
      • Retinoic acid receptor beta polypeptide antibody
      • RRB2 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
      Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
      Immunocytochemistry/ Immunofluorescence analysis of HCT 116 (Human colorectal carcinoma epithelial cell) cells labeling Retinoic Acid Receptor beta with Purified ab124701 at 1:100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling Retinoic Acid Receptor beta with Purified ab124701 at 1:100 dilution (10.3 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Western blot - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
      Western blot - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
      All lanes : Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701) at 1/2000 dilution (Purified)

      Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates
      Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 50 kDa
      Observed band size: 55 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
      Western blot - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
      All lanes : Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701) at 1/1000 dilution

      Lane 1 : MCF7 cell lysates
      Lane 2 : MDA-MB-435 cell lysates
      Lane 3 : Daudi cell lysates
      Lane 4 : U-87 MG cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lane 1 : Goat anti-Rabbit HRP at 1/20000 dilution
      Lanes 2-4 : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 50 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)

      ab124701 at 1/100 dilution staining Retinoic Acid Receptor beta in paraffin-embedded Human kidney tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
      OI-RD Scanning - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
      Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab124701? Please let us know so that we can cite the reference in this datasheet.

    ab124701 has been referenced in 4 publications.

    • Li S  et al. A novel all-trans retinoic acid derivative regulates cell cycle and differentiation of myelodysplastic syndrome cells by USO1. Eur J Pharmacol 906:174199 (2021). PubMed: 34058203
    • Wang W  et al. High Expression of RARß Is a Favorable Factor in Colorectal Cancer. Dis Markers 2019:7138754 (2019). PubMed: 30944670
    • Park J  et al. CCL28-induced RARß expression inhibits oral squamous cell carcinoma bone invasion. J Clin Invest 129:5381-5399 (2019). PubMed: 31487270
    • Greussing R  et al. Identification of microRNA-mRNA functional interactions in UVB-induced senescence of human diploid fibroblasts. BMC Genomics 14:224 (2013). WB . PubMed: 23557329

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Retinoic Acid Receptor beta antibody [EPR2017]

    Inconclusive
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Adult Testis)
    Specification
    Adult Testis
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
    Permeabilization
    No
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. 煜欽 林

    Verified customer

    Submitted Jan 17 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Retinoic Acid Receptor beta antibody [EPR2017]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Marmoset (common) Tissue sections (Adult testis)
    Specification
    Adult testis
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
    Permeabilization
    No
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. 煜欽 林

    Verified customer

    Submitted Jan 09 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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