Recombinant Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)
![Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)](https://www.abcam.com/ps/products/187/ab187159/Images/ab187159-310165-anti-retinoic-acid-receptor-gamma-antibody-epr2020n-n-terminal-western-blot.jpg "Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2020(N)] to Retinoic Acid Receptor gamma - N-terminal
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal
See all Retinoic Acid Receptor gamma primary antibodies -
Description
Rabbit monoclonal [EPR2020(N)] to Retinoic Acid Receptor gamma - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- NCCIT, NTERA2/D1 and HeLa cell lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2020(N) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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| ICC/IF |
1/100 - 1/250.
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| Notes |
|---|
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WB
1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
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ICC/IF
1/100 - 1/250. |
Target
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Function
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5916 Human
- Omim: 180190 Human
- SwissProt: P13631 Human
- Unigene: 1497 Human
- Unigene: 733399 Human
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Alternative names
- NR1B3 antibody
- Nuclear receptor subfamily 1 group B member 3 antibody
- RAR gamma antibody
see all
Images
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Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)All lanes : Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : RARG (Acid Receptor gamma) knockout HAP1 whole cell lysate
Lane 3 : A375 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 50 kDa
Observed band size: 50 kDaLanes 1 - 3: Merged signal (red and green). Green - ab187159 observed at 50 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab187159 was shown to recognize Acid Receptor gamma in wild-type HAP1 cells as signal was lost at the expected MW in RARG (Acid Receptor gamma) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and RARG (Acid Receptor gamma) knockout samples were subjected to SDS-PAGE. Ab187159 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)](https://www.abcam.com/ps/products/187/ab187159/Images/ab187159-220344-anti-retinoic-acid-receptor-gamma-antibody-epr2020n-n-terminal-western-blot.jpg)
Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)All lanes : Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159) at 1/1000 dilution
Lane 1 : NCCIT cell lysate
Lane 2 : NTERA2/D1 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 50 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)](https://www.abcam.com/ps/products/187/ab187159/Images/ab187159-220346-anti-retinoic-acid-receptor-gamma-antibody-epr2020n-n-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)Immunofluorescent analysis of HeLa cells labeling Retinoic Acid Receptor gamma with ab187159 at 1/250 and DAPI staining (blue).
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![Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)](https://www.abcam.com/ps/products/187/ab187159/Images/ab187159-4-benefits-of-recombinant-antibodies.png)
Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab187159 has been referenced in 2 publications.
- Li S et al. A novel all-trans retinoic acid derivative regulates cell cycle and differentiation of myelodysplastic syndrome cells by USO1. Eur J Pharmacol 906:174199 (2021). PubMed: 34058203
- Magdy T et al. RARG variant predictive of doxorubicin-induced cardiotoxicity identifies a cardioprotective therapy. Cell Stem Cell 28:2076-2089.e7 (2021). PubMed: 34525346